Histochemical and biochemical study of the production and function of neurosteroid
| Project/Area Number |
23590236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ISHIMURA Kazunori 徳島大学, ヘルスバイオサイエンス研究部, 教授 (90112185)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Koji 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (00548597)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 海馬 / 神経細胞 / 神経ステロイド / ステロイド合成酵素 / 免疫組織化学 / 酵素発現 / アロマターゼ / 卵巣摘除 |
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| Research Abstract |
To elucidate the physiological role of neurosteroids that are produced in the central nervous system, we analyzed the expression of steroid synthesizing enzymes in the choroid plexus with RT-PCR. The results showed that several enzymes were expressed in the choroid plexus epithelial cells. The concentration of steroid hormones in cerebrospinal fluid is much lower than that in blood plasma, the steroidogenic enzymes expressed in the choroid plexus may play roles in homeostasis of steroids in the cerebrospinal fluid. We examined the effect of ovariectomy on hippocampal neural cell death of rats which were under heavy physical stress. The results showed that the nerve cells decreased in number by the stress in ovariectomized Fisher rat, but not in Wistar ones. There might be a lineage difference for neuronal cell viability in the rat under a combination of stress and estrogen deficiency.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Nucling, a novel protein associated with NF-・B, regulates endotoxin-induced apoptosis in vivo2013
Author(s)
Sun Mi Kim, Sakai, T., Huy Van Dang, Nam Hoang Tran, Ono, K., Ishimura, K. and Fukui, K
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Journal Title
J. Biochem
Volume: Vol. 153、No. 1
Issue: 1
Pages: 93-101
DOI
Related Report
Peer Reviewed
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