Transcriptional mechanism for maintenance and differentiation of neural stem cells

• Project/Area Number: 23590237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: TAKEBAYASHI HIROHIDE 新潟大学, 医歯学系, 教授 (60353439)
• Research Collaborator: USUI Noriyoshi ; BEPARI Asim
• Project Period (FY): 2011-04-28 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オリゴデンドロサイト / 分化 / ミエリン / コンディショナルノックアウトマウス / 転写因子 / Olig2 / リン酸化 / 結合因子

Outline of Final Research Achievements

Oligodenrocytes are glial cells in the central nervous system which are produced from neural stem cells during neural development. Olig2 is an essential transcription factor for oligodendrocyte development.In this study, we screened Olig2-binding proteins. We identified Olig2 binding proteins (OBP1 and OBP2). Overexpression experiment of OBP2 in embryonic mouse brain resulted in elevated expression of myelin-related genes. In addition, generation of central nervous system (CNS)-specific conditional knockout mice resulted in postnatal lethality with CNS abnormality.

Research Products

• [Journal Article] Expression of the Olig gene family in the developing mouse inner ear (2015)
  • Author(s): Kanaya E, Yamahara K, Okano T, Yoshida A, Katsuno T, Takebayashi H, Ito J, Yamamoto N
  • Journal Title: Gene Expr Patterns Volume: 17 Issue: 2 Pages: 79-86
  • DOI: 10.1016/j.gep.2015.03.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of the prethalamus is crucial for thalamocortical projection formation and is regulated by Olig2. (2014)
  • Author(s): Ono K., Clavairoly A., Nomura T., Gotoh H., Uno A., Armant O., Takebayashi H., Zhang Q., Shimamura K., Itohara S., Parras C.M., Ikenaka K.
  • Journal Title: Development Volume: 141 Issue: 10 Pages: 2075-2084
  • DOI: 10.1242/dev.097790
  • Peer Reviewed / Open Access
• [Journal Article] Disruption of actin-binding domain-containing Dystonin protein causes dystonia musculorum in mice. (2014)
  • Author(s): Horie, M., Watanabe, K., Bepari, A. K., Nashimoto, J., Araki, K., Sano, H., Chiken, S., Nambu, A., Ono, K., Ikenaka, K., Kakita, A., Yamamura, K. and Takebayashi, H.
  • Journal Title: Eur. J Neurosci. Volume: 40 Issue: 10 Pages: 3458-3471
  • DOI: 10.1111/ejn.12711
  • Peer Reviewed
• [Journal Article] グリア細胞発生・分化機構の解析と生体内細胞系譜追跡実験系の確立・応用 (2014)
  • Author(s): 竹林浩秀
  • Journal Title: 神経化学 Volume: 53 Pages: 41-46
• [Journal Article] Olig2-lineage cells preferentially differentiate into oligodendrocytes but their processes degenerate at the chronic demyelinating stage of proteolipid protein-overexpressing mouse. (2013)
  • Author(s): Shimizu T, Tanaka KF, Takebayashi H, Higashi M, Wisesmith W, Ono K, Hitoshi S, Ikenaka K.
  • Journal Title: J Neurosci Res Volume: 91 Pages: 178-186
  • Peer Reviewed
• [Journal Article] Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation (2012)
  • Author(s): Usui N, et al
  • Journal Title: Development Volume: 139 Issue: 6 Pages: 1125-32
  • DOI: 10.1242/dev.069997
  • Peer Reviewed
• [Journal Article] Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche. (2012)
  • Author(s): Lee DA, Bedont JL, Pak T, Wang H, Song J, Miranda-Angulo A, Takiar V, Charubhumi V, Balordi F, Takebayashi H, Aja S, Ford E, Fishell G, Blackshaw S.
  • Journal Title: Nat Neurosci Volume: 15 Issue: 5 Pages: 700-702
  • DOI: 10.1038/nn.3079
  • Peer Reviewed
• [Journal Article] Visualization of odor-induced neuronal activity by immediate early gene expression. (2012)
  • Author(s): Bepari AK, Watanabe K, Yamaguchi M, Tamamaki N, Takebayashi H.
  • Journal Title: BMC Neurosci Volume: 13 Issue: 1 Pages: 140-140
  • DOI: 10.1186/1471-2202-13-140
  • Peer Reviewed
• [Journal Article] Nkx2.2+ progenitors generate somatic motoneurons in the chick spinal cord. (2012)
  • Author(s): Gotoh H, Ono K, Nomura T, Takebayashi H, Harada H, Nakamura H, Ikenaka K.
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: e51581-e51581
  • DOI: 10.1371/journal.pone.0051581
  • Peer Reviewed
• [Journal Article] Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis. (2011)
  • Author(s): Guo F et al.
  • Journal Title: Journal of Neuroscience Volume: 31 Issue: 33 Pages: 11914-11928
  • DOI: 10.1523/jneurosci.1759-11.2011
  • Peer Reviewed
• [Journal Article] Olig2 lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta, ventral lateral geniculate nucleus and reticular thalamic nucleus. (2011)
  • Author(s): Inamura N et al.
  • Journal Title: Developmental Neuroscience Volume: 33 Issue: 2 Pages: 118-129
  • DOI: 10.1159/000328974
  • Peer Reviewed
• [Journal Article] Dpy19l1, a multi-transmembrane protein, regulates the radial migration of glutamatergic neurons in the developing cerebral cortex. (2011)
  • Author(s): Watanabe K et al.
  • Journal Title: Development Volume: 138 Issue: 22 Pages: 4979-4990
  • DOI: 10.1242/dev.068155
  • Peer Reviewed
• [Presentation] 脳のできるしくみから学ぶ (2015)
  • Author(s): 竹林浩秀
  • Organizer: 第１６回 日本早期認知症学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-10-10
  • Invited
• [Presentation] Analyses of neuronal and glial cell phenotypes of dystonia musculorum mice (2015)
  • Author(s): Hirohide Takebayashi
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: ソニックシティ（埼玉県大宮市）
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] Mouse model of dystonia with sensory neuropathy (2015)
  • Author(s): Hirohide Takebayashi
  • Organizer: 解剖生理合同大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] Oligodendrogenesis in the adult brain and abnormal proliferation of oligodendrocyte progenitor cells in neuropathological condition. (2015)
  • Author(s): Hirohide Takebayashi
  • Organizer: 新学術領域グリアアセンブリ 国際シンポジウム
  • Place of Presentation: 東京大学 伊藤謝恩ホール
  • Year and Date: 2015-01-23
  • Invited
• [Presentation] ジストニア症状を示す遺伝性神経難病モデルマウスの作製と解析 (2014)
  • Author(s): 竹林浩秀
  • Organizer: 第703回 新潟医学会
  • Place of Presentation: 有壬記念館（新潟市）
  • Year and Date: 2014-11-15
  • Invited
• [Presentation] グリア細胞発生・分化機構の解析と生体内細胞系譜追跡実験系の確立・応用 (2014)
  • Author(s): 竹林浩秀
  • Organizer: 日本神経化学会
  • Place of Presentation: 奈良県文化会館
  • Year and Date: 2014-09-29 – 2014-10-01
  • Invited
• [Presentation] グリア細胞の発生について (2013)
  • Author(s): 竹林浩秀
  • Organizer: 第37回峠の会
  • Place of Presentation: 福島
  • Invited
• [Presentation] Npas4 mRNA expression can trace photoactivation of striatal mediunm spiny neurons at the cellular level (2013)
  • Author(s): 竹林浩秀、ベパリ アシム、佐野裕美、玉巻伸章、南部篤、田中謙二
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川
• [Presentation] Regulation of oligodendrogenesis by phosphoprotein Olig2 (2012)
  • Author(s): Takebayashi H
  • Organizer: Gordon Research Conference: Myelin
  • Place of Presentation: Lucca, Italy
  • Invited
• [Presentation] ニューロン・グリアの分化メカニズム研究と今後の研究展開 (2012)
  • Author(s): 竹林浩秀
  • Organizer: 第５回 神経発生討論会(招待講演)
  • Place of Presentation: 福井県県民ホール（福井市）
• [Remarks] 新潟大学医歯学総合研究科 神経性物・解剖学分野
  • URL: http://www.med.niigata-u.ac.jp/an2/index.html
• [Remarks] 新潟大学大学院 医歯学総合研究科 神経生物・解剖学分野
  • URL: http://www.med.niigata-u.ac.jp/an2/index.html
• [Remarks]
  • URL: http://www.neurochemistry.jp/mutms0k7g-15/#_15