| Project/Area Number |
23590241
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
齋野 朝幸 , 准教授 (40305991)
東尾 浩典 , 助教 (50342837)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
松浦 誠 , 講師 (00405846)
葉原 芳昭 , 教授 (30142813)
石田 陽治 , 教授 (70151389)
永井 健治 , 教授 (20311350)
小笠原 邦昭 , 教授 (00305989)
阿久津 仁美 , 助教 (30398482)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 解剖学 / 細胞・組織 / シグナル伝達 / 生理活性 / 脂肪酸ｴﾁﾙｴｽﾃﾙ / 脂肪酸エチルエステル / 細胞内カルシウム濃度 / 膵腺房細胞 / 血球 / 細静脈 / 高速共焦点レーザー顕微鏡 / マスト細胞 / 細胞死イメージング |
|---|
| Research Abstract |
To investigate whether free fatty acid ethylesters (FAEEs) can induce cell damage, we observed intracellular [Ca2+] dynamics in peritoneal cells, pancreatic acinar cells and isolated vascular specimens. Palmitoleic acid ethyl ester might induce slight invrease of [Ca2+]i in some peritoneal cells, and Oleic acid ethyl wster caused [Ca2+]i increase in pancreatic acinus. In pacreatic specimens, dead cells increase in number after FAEEs-perfusion, however the increase cannot statistically verified. FAEEs were dissolved in DMSO, however the solvent can induce cell damage. In conclusion, in the experiment, we cannot detect any evidences of FAEEs' effect on intracellular signalling.
|
