Roles of basement membrane-actin cytoskeletal system on the epithelial branching of embryonic submandibular gland of mouse
| Project/Area Number |
23590243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
KADOYA Yuichi 北里大学, 医療衛生学部, 教授 (10185887)
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| Project Period (FY) |
2011-04-28 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 唾液腺 / 分枝形態形成 / 細胞骨格 / 焦点結合 / ライブイメージング / 顎下腺 / 組織形成 / フォーカルアドヒージョン / 非筋ミオシンII / 細胞運動 / 器官培養 / ミオシン |
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| Outline of Final Research Achievements |
I attempted to clarify roles of the basement membrane-actin cytoskeleton system for the epithelial branching morphogenesis. Submandibular rudiments from the embryonic day-13 mice were dissected and cultured. Some rudiments were further treated with dispase and the epithelia were separated. Then they were monolayer-cultured on the iMatrix511-coated culture dish, or embedded within the Matrigel. Effects of a myosin II inhibitor, blebbistatin, and a focal adhesion kinase inhibitor, PF573228, were tested in these culture conditions. The phosphorylation of the focus adhesion kinase was found to play roles for the salivary gland branching morphogenesis. A suppressive role of the myosin II was also suggested for controlling the clefting. However, mechanism controlling the initiation and elongation of cleft remained for the future studies.
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Report
(6 results)
Research Products
(8 results)
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[Presentation] 腎組織形成における細胞の動態2016
Author(s)
佐々木政嘉, 門谷裕一
Organizer
第121回日本解剖学会総会全国学術集会
Place of Presentation
郡山市（福島県）、ビッグ パレットふくしま
Year and Date
2016-03-28
Related Report
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