| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
I attempted to clarify roles of the basement membrane-actin cytoskeleton system for the epithelial branching morphogenesis. Submandibular rudiments from the embryonic day-13 mice were dissected and cultured. Some rudiments were further treated with dispase and the epithelia were separated. Then they were monolayer-cultured on the iMatrix511-coated culture dish, or embedded within the Matrigel. Effects of a myosin II inhibitor, blebbistatin, and a focal adhesion kinase inhibitor, PF573228, were tested in these culture conditions. The phosphorylation of the focus adhesion kinase was found to play roles for the salivary gland branching morphogenesis. A suppressive role of the myosin II was also suggested for controlling the clefting. However, mechanism controlling the initiation and elongation of cleft remained for the future studies.
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