| Project/Area Number |
23590245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 周皮細胞 / 細胞シート工学 / 基底膜 / 血管新生 / GFP / 多重免疫染色 / 電子顕微鏡 / レクチン / 組織学 / 移植 / IMARIS / 内皮細胞 / 免疫組織化学 |
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| Outline of Final Research Achievements |
A high viability of grafted cell sheets is likely due to a sufficient blood circulation between grafts and hosts. However, the cellular mechanism of the sufficient circulation is still enigmatic. In this study, we used the cell sheets fabricated by rat heart wall, which contain vascular endothelial cells beforehand and usually develop intrinsic vascular network before grafting on host rat muscles. Early after grafting, blood inflow from the hosts into the grafts was confirmed by a tracer experiment. Vascular anastomosis between hosts and grafts was also observed. The anastomosed vessels were already distributed with pericytes and entirely covered by a vascular basement membrane. Moreover, it turned out that the pericytes had an ability to produce vascular basement membrane constituents. We conclude that pericytes promote vessels of hosts and grafts by producing vascular basement membrane, that functions as a scaffold for migrating vascular endothelial cells to anastomose each other.
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