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Investigation of trans-interaction between claudins, tight junction constitutive transmembrane proteins, and paracellular permeability.

Research Project

Project/Area Number 23590249
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionFukuoka Dental College

Principal Investigator

INAI Tetsuichiro  福岡歯科大学, 口腔歯学部, 教授 (00264044)

Co-Investigator(Kenkyū-buntansha) 廣瀬 英司  明治国際医療大学, 公私立大学の部局等, 准教授 (40380620)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordstight junction / claudin / trans-interaction / permeability / タイト結合 / クローディン / 細胞間透過性
Research Abstract

We established HEK293 cells, which does not form tight junctions (TJs), expressing RFP-cldn-1, RFP-cldn-10b, EGFP-cldn-10a or EGFP-cldn-10b. EGFP-cldn-10a- and RFP-cldn-1-expressing cells, EGFP-cldn-10b- and RFP-cldn-1-expressing cells, or EGFP-cldn-10a- and RFP-cldn-10b-expressing cells were co-cultured and examined by confocal laser scanning microscopy. Both EGFP-cldn-10a and EGFP-cldn-10b were not co-localized with RFP-cldn-1 in cell-cell contacts between heterotypic cells. In contrast, EGFP-cldn-10a was co-localized with RFP-cldn-10b. Using freeze-fracture electron microscopy, P-face-associated or E- face-associated TJ particles were observed in EGFP-cldn-10a- or EGFP-cldn-10b-expressing cells, respectively. When cldn-10a or cldn-10b was expressed in TJ-bearing MDCK I cells, cldn-10a did not alter trans-epithelial electrical resistance (TER), but cldn-10b markedly reduced TER.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Effects of bisphosphonate administration on peri-implant bone in vitamin D-deficient rats2013

    • Author(s)
      Wang D, Beppu K, Yamamoto K, Inai T, Kido H
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 22(1) Pages: 79-88

    • NAID

      130004848414

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Inhibition of extracellular signal-regulated kinase downregulates claudin-2 expression and alters paracellular permeability in mouse rectum CMT93-II cells.2013

    • Author(s)
      Inai T, Kitagawa N, Hatakeyama Y, Ikebe T, Iida H, Fujita M.
    • Journal Title

      Tissue and Cell

      Volume: 45 Issue: 3 Pages: 175-182

    • DOI

      10.1016/j.tice.2012.11.001

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Resurgent-like currents in mouse vas deferens myocytes are mediated by NaV1.6 voltage-gated sodium channels.2012

    • Author(s)
      Teramoto N, Zhu HL, Yotsu-Yamashita M, Inai T, Cunnane TC
    • Journal Title

      Pflugers Arch.

      Volume: 464 Issue: 5 Pages: 493-502

    • DOI

      10.1007/s00424-012-1153-4

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner's Glands by Ion Etching-Immunoscanning Electron Microscopy2011

    • Author(s)
      Yahiro J, Inai T, Tsutsui A, Sato A, Nagato T, Taniguchi K, Tsuruga E, Sawa Y
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 44 Issue: 5 Pages: 201-212

    • DOI

      10.1267/ahc.10039

    • NAID

      130000941525

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Growth differentiation factor 5 (GDF-5) induces matrix metalloproteinase 2 (MMP-2) expression in periodontal ligament cells and modulates MMP-2 and MMP-13 activity in osteoblasts2011

    • Author(s)
      Hatakeyama Y, Hatakeyama J, Maruya Y, Oka K, Tsuruga E, Inai T, Sawa Y
    • Journal Title

      Bone and Tissue Regeneration Insights

      Volume: 4 Pages: 1-10

    • Related Report
      2013 Final Research Report
  • [Journal Article] The effect of valproic Acid on mesenchymal pluripotent cell proliferation and differentiation in extracellular matrices2011

    • Author(s)
      Hatakeyama Y, Hatakeyama J, Takahashi A, Oka K, Tsuruga E, Inai T, Sawa Y
    • Journal Title

      Drug Target Insights

      Volume: 5 Pages: 1-9

    • DOI

      10.4137/dti.s6534

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] The coculture method to examine interactions between claudin isoforms in tight junction-free HEK293 cells and tight junction-bearing MDCK II cells2011

    • Author(s)
      Inai T
    • Journal Title

      Methods Mol Biol

      Volume: 762 Pages: 101-14

    • DOI

      10.1007/978-1-61779-185-7_8

    • ISBN
      9781617791840, 9781617791857
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Immunolocalization of Spetex-1 at the Connecting Piece in Spermatozoa of the Musk Shrew (Suncus murinus)2011

    • Author(s)
      Kaneko, et al
    • Journal Title

      Zoological Science

      Volume: 28 Issue: 6 Pages: 444-452

    • DOI

      10.2108/zsj.28.444

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Growth Differentiation Factor 5 (GDF-5) Induces Matrix Metalloproteinase 2 (MMP-2) Expression in Periodontal Ligament Cells and Modulates MMP-2 and MMP-13 Activity in Osteoblasts2011

    • Author(s)
      Hatakeyama Y, Hatakeyama J, Maruya Y, Oka K, Tsuruga E, Inai T, Sawa Y
    • Journal Title

      Bone and Tissue Regeneration Insights

      Volume: 4 Pages: 1-10

    • DOI

      10.4137/btri.s8120

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] JNK阻害剤による表皮角化細胞由来HaCaT細胞の分化誘導と分化マーカーの解析2014

    • Author(s)
      北河憲雄、畠山雄次、稲井哲一朗
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学キャンパス
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] タイト結合構成膜蛋白claudin-1の細胞外第2ループの機能解析2013

    • Author(s)
      稲井哲一朗、北河憲雄、畠山雄次
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      サンポートホール高松
    • Related Report
      2012 Research-status Report
  • [Presentation] タイト結合構成膜蛋白claudin-1の細胞外第2ループの機能解析2012

    • Author(s)
      稲井哲一朗、北河憲雄、畠山雄次
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞間接着装置-膜蛋白の解析から見えてくるもの-タイト結合膜蛋白claudinの相互作用とタイト結合の形態形成2011

    • Author(s)
      稲井哲一朗
    • Organizer
      日本顕微鏡学会第67回学術講演会
    • Place of Presentation
      日福岡国際会議場
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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