Ion channel coupling and membrane plasticity in nucleus and mitochondria

• Project/Area Number: 23590254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: MARUYAMA Yoshio 東北大学, 医学(系)研究科(研究院), 教授 (00133942)
• Co-Investigator(Kenkyū-buntansha): MURATA Yoshimichi 東北大学, 大学院医学系研究科, 講師 (60455780) ; KAZAMA Itsurou 東北大学, 大学院医学系研究科, 助教 (60593978)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 核膜・小胞体膜 / イオンチャネル / 膜容量 / 膜可塑性 / 膵外分泌腺 / 核膜空間 / レーザー顕微鏡 / 核膜イオンチャネル / 核膜の可塑性

Research Abstract

Fixing the lumen Ca2+-concentration at over 10 micro molar concentration in a nuclear/endoplasmic reticulum preparation (nuclear envelope: NE), which is subjected into whole-NE configuration of the patch-clamp recording techniques, I observed increases of membrane electrical capacitance, the measure of the membrane area. The membrane capacitance, recorded under in situ patch-clamp condition, fluctuated microscopically. The result suggests that the NE membrane system shows a characteristic undulation reflecting temporal membrane vesiculation and collapse, and the process is under control of the lumen Ca2+ concentration. The NE membrane is rich in ion- and water- channels. Those active membrane may component contribute to maintain NE assembly, the structure of the lumen and the nuclear membranes. Further, I observed with laser confocal microscopy in the NE preparation, a thick membrane accumulation, stacking into layers at a small spot, assumingly corresponding to the nucleic pore.

Research Products

• [Journal Article] Flagellin/TLR5 signaling potentiates airway serous secretion from swine tracheal submucosal glands (2013)
  • Author(s): Soshi Muramatsu, Tsutomu Tamada, Masayuki Nara, Koji Murakami, Toshiaki Kikuchi, Masahiko Kanehira, Yoshio Maruyama, Masahito Ebina, Toshihiro Nukiwa and Masakazu Ichinose
  • Journal Title: Am.J. Physiol Volume: 305
  • Peer Reviewed
• [Journal Article] Decreased Expression of a Novel Prostaglandin Transporter, OAT-PG, Facilitates Renocortical PGE(2) Accumulation during Rat Pregnancy (2013)
  • Author(s): Kazama I, Matsubara M, Kanai Y, Hatano R, Asano S, Endo Y, Toyama H, Ejima Y, Kurosawa S, Maruyama Y
  • Journal Title: Gynecol Obstet Invest Volume: 76 Issue: 3 Pages: 163-170
  • DOI: 10.1159/000353977
  • Peer Reviewed
• [Journal Article] Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis (2013)
  • Author(s): Kazama I, Maruyama Y, Takahashi S, Kokumai T
  • Journal Title: Cell Physiol Biochem Volume: 31(4-5) Issue: 4-5 Pages: 592-600
  • DOI: 10.1159/000350079
  • Peer Reviewed
• [Journal Article] Differential effects of clarithromycin and azithromycin on delayed rectifier K(+)-channel currents in murine thymocytes (2013)
  • Author(s): Kazama I, Maruyama Y
  • Journal Title: Pharm Biol Volume: 51(6) Issue: 6 Pages: 760-5
  • DOI: 10.3109/13880209.2013.764539
  • Peer Reviewed
• [Journal Article] Flagellin/TLR5 signaling potentiates airway serous secretion from swine trachal submucosal glands (2013)
  • Author(s): Soshi Muramatsu, Tsutomu Tamada, Maayuki Nara, Toshiaki Kikuchi, Masahiko Kanehira, Yoshio Maruyama, Maahito Ebina, Toshihiko Nukiwa, Masakazu Ichinose
  • Journal Title: American journal of Physiology Lung Cell mol Physiol Volume: 305
  • Peer Reviewed
• [Journal Article] Aspirin-Induced Microscopic Surface Changes Stimulate Thrombopoiesis in Rat Megakaryocytes (2012)
  • Author(s): Kazama I, Maruyama Y, Nakamichi S
  • Journal Title: Clin Appl Thromb Hemost Volume: - Issue: 3 Pages: 318-325
  • DOI: 10.1177/1076029612461845
  • Peer Reviewed
• [Journal Article] Benidipine persistently inhibits delayed rectifier K(+)-channel currents in murine thymocytes (2012)
  • Author(s): Kazama I, Maruyama Y, Matsubara M
  • Journal Title: Immunopharmacol Immunotoxicol Volume: 35(1) Issue: 1 Pages: 28-33
  • DOI: 10.3109/08923973.2012.723011
  • Peer Reviewed
• [Journal Article] Inner mitochondrial maxi-K⁺channels in neonatal renal tubular cells : novel therapeutic targets to control apoptosis (2012)
  • Author(s): Kazama I, Maruyama Y
  • Journal Title: Med Hypotheses Volume: 78(6) Issue: 6 Pages: 800-1
  • DOI: 10.1016/j.mehy.2012.03.013
  • Peer Reviewed
• [Journal Article] Suppressive effects of nonsteroidal anti-inflammatory drugs diclofenac sodium, salicylate and indomethacin on delayed rectifier K+-channel currents in murine thymocytes (2012)
  • Author(s): Kazama I, Maruyama Y, Murata Y
  • Journal Title: Immunopharmacol Immunotoxicol Volume: 34(5) Issue: 5 Pages: 874-8
  • DOI: 10.3109/08923973.2012.666249
  • Peer Reviewed
• [Journal Article] Voltage-dependent biphasic effects of chloroquine on delayed rectifier K^+-channel currents in murine thymocytes (2012)
  • Author(s): 風間逸郎、丸山芳夫、村田喜理
  • Journal Title: Journal of Physiological Science Volume: (印刷中) Issue: 3 Pages: 267-274
  • DOI: 10.1007/s12576-012-0195-x
  • Peer Reviewed
• [Journal Article] Inner mitochondorial Maxi-K channels in neonatal renal tubular cells: Novel therapeutic targets to control apoptosis (2012)
  • Author(s): Itsuro Kazama and Yoshio Maruyama
  • Journal Title: Medical Hypotheses Volume: 78 Pages: 8000-8001
  • Peer Reviewed