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Ion channel coupling and membrane plasticity in nucleus and mitochondria

Research Project

Project/Area Number 23590254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionTohoku University

Principal Investigator

MARUYAMA Yoshio  東北大学, 医学(系)研究科(研究院), 教授 (00133942)

Co-Investigator(Kenkyū-buntansha) MURATA Yoshimichi  東北大学, 大学院医学系研究科, 講師 (60455780)
KAZAMA Itsurou  東北大学, 大学院医学系研究科, 助教 (60593978)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords核膜・小胞体膜 / イオンチャネル / 膜容量 / 膜可塑性 / 膵外分泌腺 / 核膜空間 / レーザー顕微鏡 / 核膜イオンチャネル / 核膜の可塑性
Research Abstract

Fixing the lumen Ca2+-concentration at over 10 micro molar concentration in a nuclear/endoplasmic reticulum preparation (nuclear envelope: NE), which is subjected into whole-NE configuration of the patch-clamp recording techniques, I observed increases of membrane electrical capacitance, the measure of the membrane area. The membrane capacitance, recorded under in situ patch-clamp condition, fluctuated microscopically. The result suggests that the NE membrane system shows a characteristic undulation reflecting temporal membrane vesiculation and collapse, and the process is under control of the lumen Ca2+ concentration. The NE membrane is rich in ion- and water- channels. Those active membrane may component contribute to maintain NE assembly, the structure of the lumen and the nuclear membranes. Further, I observed with laser confocal microscopy in the NE preparation, a thick membrane accumulation, stacking into layers at a small spot, assumingly corresponding to the nucleic pore.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012

All Journal Article (11 results) (of which Peer Reviewed: 11 results)

  • [Journal Article] Flagellin/TLR5 signaling potentiates airway serous secretion from swine tracheal submucosal glands2013

    • Author(s)
      Soshi Muramatsu, Tsutomu Tamada, Masayuki Nara, Koji Murakami, Toshiaki Kikuchi, Masahiko Kanehira, Yoshio Maruyama, Masahito Ebina, Toshihiro Nukiwa and Masakazu Ichinose
    • Journal Title

      Am.J. Physiol

      Volume: 305

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased Expression of a Novel Prostaglandin Transporter, OAT-PG, Facilitates Renocortical PGE(2) Accumulation during Rat Pregnancy2013

    • Author(s)
      Kazama I, Matsubara M, Kanai Y, Hatano R, Asano S, Endo Y, Toyama H, Ejima Y, Kurosawa S, Maruyama Y
    • Journal Title

      Gynecol Obstet Invest

      Volume: 76 Issue: 3 Pages: 163-170

    • DOI

      10.1159/000353977

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis2013

    • Author(s)
      Kazama I, Maruyama Y, Takahashi S, Kokumai T
    • Journal Title

      Cell Physiol Biochem

      Volume: 31(4-5) Issue: 4-5 Pages: 592-600

    • DOI

      10.1159/000350079

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential effects of clarithromycin and azithromycin on delayed rectifier K(+)-channel currents in murine thymocytes2013

    • Author(s)
      Kazama I, Maruyama Y
    • Journal Title

      Pharm Biol

      Volume: 51(6) Issue: 6 Pages: 760-5

    • DOI

      10.3109/13880209.2013.764539

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flagellin/TLR5 signaling potentiates airway serous secretion from swine trachal submucosal glands2013

    • Author(s)
      Soshi Muramatsu, Tsutomu Tamada, Maayuki Nara, Toshiaki Kikuchi, Masahiko Kanehira, Yoshio Maruyama, Maahito Ebina, Toshihiko Nukiwa, Masakazu Ichinose
    • Journal Title

      American journal of Physiology Lung Cell mol Physiol

      Volume: 305

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aspirin-Induced Microscopic Surface Changes Stimulate Thrombopoiesis in Rat Megakaryocytes2012

    • Author(s)
      Kazama I, Maruyama Y, Nakamichi S
    • Journal Title

      Clin Appl Thromb Hemost

      Volume: - Issue: 3 Pages: 318-325

    • DOI

      10.1177/1076029612461845

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Benidipine persistently inhibits delayed rectifier K(+)-channel currents in murine thymocytes2012

    • Author(s)
      Kazama I, Maruyama Y, Matsubara M
    • Journal Title

      Immunopharmacol Immunotoxicol

      Volume: 35(1) Issue: 1 Pages: 28-33

    • DOI

      10.3109/08923973.2012.723011

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inner mitochondrial maxi-K⁺channels in neonatal renal tubular cells : novel therapeutic targets to control apoptosis2012

    • Author(s)
      Kazama I, Maruyama Y
    • Journal Title

      Med Hypotheses

      Volume: 78(6) Issue: 6 Pages: 800-1

    • DOI

      10.1016/j.mehy.2012.03.013

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive effects of nonsteroidal anti-inflammatory drugs diclofenac sodium, salicylate and indomethacin on delayed rectifier K+-channel currents in murine thymocytes2012

    • Author(s)
      Kazama I, Maruyama Y, Murata Y
    • Journal Title

      Immunopharmacol Immunotoxicol

      Volume: 34(5) Issue: 5 Pages: 874-8

    • DOI

      10.3109/08923973.2012.666249

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Voltage-dependent biphasic effects of chloroquine on delayed rectifier K^+-channel currents in murine thymocytes2012

    • Author(s)
      風間逸郎、丸山芳夫、村田喜理
    • Journal Title

      Journal of Physiological Science

      Volume: (印刷中) Issue: 3 Pages: 267-274

    • DOI

      10.1007/s12576-012-0195-x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inner mitochondorial Maxi-K channels in neonatal renal tubular cells: Novel therapeutic targets to control apoptosis2012

    • Author(s)
      Itsuro Kazama and Yoshio Maruyama
    • Journal Title

      Medical Hypotheses

      Volume: 78 Pages: 8000-8001

    • Related Report
      2012 Research-status Report
    • Peer Reviewed

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Published: 2011-08-05   Modified: 2019-07-29  

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