| Project/Area Number |
23590254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Tohoku University |
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Principal Investigator |
MARUYAMA Yoshio 東北大学, 医学(系)研究科(研究院), 教授 (00133942)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yoshimichi 東北大学, 大学院医学系研究科, 講師 (60455780)
KAZAMA Itsurou 東北大学, 大学院医学系研究科, 助教 (60593978)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 核膜・小胞体膜 / イオンチャネル / 膜容量 / 膜可塑性 / 膵外分泌腺 / 核膜空間 / レーザー顕微鏡 / 核膜イオンチャネル / 核膜の可塑性 |
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| Research Abstract |
Fixing the lumen Ca2+-concentration at over 10 micro molar concentration in a nuclear/endoplasmic reticulum preparation (nuclear envelope: NE), which is subjected into whole-NE configuration of the patch-clamp recording techniques, I observed increases of membrane electrical capacitance, the measure of the membrane area. The membrane capacitance, recorded under in situ patch-clamp condition, fluctuated microscopically. The result suggests that the NE membrane system shows a characteristic undulation reflecting temporal membrane vesiculation and collapse, and the process is under control of the lumen Ca2+ concentration. The NE membrane is rich in ion- and water- channels. Those active membrane may component contribute to maintain NE assembly, the structure of the lumen and the nuclear membranes. Further, I observed with laser confocal microscopy in the NE preparation, a thick membrane accumulation, stacking into layers at a small spot, assumingly corresponding to the nucleic pore.
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