New synthetic peptide enhances regeneration of tissues

• Project/Area Number: 23590268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Kinki University
• Principal Investigator: OKADA Kiyotaka 近畿大学, 医学部, 講師 (20185432)
• Co-Investigator(Kenkyū-buntansha): MATSUO Osamu 近畿大学, 医学部, 顧問 (40030879) ; KAWAO Naoyuki 近畿大学, 医学部, 助教 (70388510)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ペプチド / 創傷治癒 / プラスミノゲン / bFGF / 線溶系 / 線維芽細胞 / plasminogen / プラスミノーゲン / 蛋白分解活性 / 塩基性線維芽細胞増殖因子 / 組織再生

Research Abstract

A new synthetic nonadecapeptide corresponding to Glu22-Leu40 of staphylokinase
molecule bound to plasminogen (Plg) and enhanced the activation of Plg by Plg activator (PA). When new synthetic peptide and bFGF were administrated in mouse skin wound healing model, acceleration of skin healing was observed than in mice with vehicle administration. Thus, new synthetic peptide enhanced Plg
activation and induced effective tissue repair.

Research Products

• [Journal Article] Influence of diabetic state and vitamin D deficiency on bone repair in female mice (2014)
  • Author(s): Mao L, Tamura Y, Kawao N, Okada K, Yano M, Okumoto K, Kaji H
  • Journal Title: Bone Volume: 61 Pages: 102-108
  • DOI: 10.1016/j.bone.2013.12.024
  • Peer Reviewed
• [Journal Article] Lack of both α2-antiplasmin and plasminogen activator inhibitor type-1 induces high IgE production (2013)
  • Author(s): Okada K, Ueshima S, Kawao N, Yano M, Tamura Y, Tanaka M, Sakamoto A, Hatano M, Arima M, Miyata S, Nagai N, Tokuhisa T, and Matsuo O
  • Journal Title: Life Sci Volume: 93 Issue: 2-3 Pages: 89-95
  • DOI: 10.1016/j.lfs.2013.05.023
  • Peer Reviewed
• [Journal Article] TGF-β_2 promotes RPE cell invasion into a collagen gel by mediating urokinase-type plasminogen activator (uPA) expression (2013)
  • Author(s): Sugioka K, Kodama A, Okada K, Iwata M, Yoshida K, Kusaka S, Matsumoto C, Kaji H, Shimomura Y
  • Journal Title: Exp Eye Res Volume: 115 Pages: 13-21
  • DOI: 10.1016/j.exer.2013.06.020
  • Peer Reviewed
• [Journal Article] Plasminogen plays a crucial role in bone repair (2013)
  • Author(s): Kawao N, Tamura Y, Okumoto K, Yano M, Okada K, Matsuo O. Kaji H
  • Journal Title: J Bone Miner Res Volume: 28 Issue: 7 Pages: 1561-1574
  • DOI: 10.1002/jbmr.1921
  • Peer Reviewed
• [Journal Article] Alpha2-antiplasmin regulates the development of dermal fibrosis by PGF(2) a synthesis through ATGL/iPLA(2). (2013)
  • Author(s): Kanno Y, Kawashita E, Kokada A, Okada K, Ueshima S, Matsuo O, Matsuno H.
  • Journal Title: Arthritis Rheum Volume: 65 Issue: 2 Pages: 492-502
  • DOI: 10.1002/art.37767
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator and plasminogen mediate activation of macrophage phagocytosis during liver repair in vivo. (2012)
  • Author(s): Kawao N, Nagai N, Tamura Y, Horiuchi Y, Okumoto K, Okada K, Suzuki Y, Umemura K, Yano M, Ueshima S, Kaji H, Matsuo O.
  • Journal Title: Thromb Haemost Volume: 107 Issue: 04 Pages: 749-59
  • DOI: 10.1160/th11-08-0567
  • Peer Reviewed
• [Journal Article] Involvement of Plasminogen Activator Inhibitor-1 in the Pathogenesis of Atopic Cataracts (2012)
  • Author(s): Hori K, Matsuda A, et al
  • Journal Title: Invest Ophthalmol Vis Sci Volume: (In Press) Issue: 4 Pages: 1846-1851
  • DOI: 10.1167/iovs.11-8380
  • Peer Reviewed
• [Journal Article] Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function (2011)
  • Author(s): Kanno Y, Ishisaki A, Nakajima K, Nishihara T, Toyoshima T, Okada K, Ueshima S, Matsushita K, Matsuo O
  • Journal Title: J Biol Chem Volume: 286 Issue: 11 Pages: 8952-8960
  • DOI: 10.1074/jbc.m110.152181
  • Peer Reviewed
• [Journal Article] A synthetic peptide derived from staphylokinase enhances plasminogen activator by tissue-type plasminogen activator. (2011)
  • Author(s): Okada K, Ueshima S, Matsuno H, Nagai N, Kawao N, Tanaka M, Matsuo O.
  • Journal Title: J Thromb Haemost Volume: 9 Issue: 5 Pages: 997-1006
  • DOI: 10.1111/j.1538-7836.2011.04257.x
  • Peer Reviewed
• [Journal Article] A synthetic peptide derived from staphylokinase enhances plasminogen activator by tissue-type plasminogen activator: (2011)
  • Author(s): Okada K.
  • Journal Title: J Thromb Haemost Volume: 9 Pages: 997-1006
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator contributes to heterogeneity of macrophages at the border of damaged site during liver repair in mice. (2011)
  • Author(s): Kawao N.
  • Journal Title: Thromb Haemost Volume: 105 Pages: 892-900
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator contributes to heterogeneity of macrophages at the border of damaged site during liver repair in mice. (2010)
  • Author(s): Kawao N, Nagai N, Tamura Y, Okada K, Yano M, Suzuki Y, Umemura K, Ueshima S, Matsuo O.
  • Journal Title: Thromb Haemost Volume: 105 Issue: 05 Pages: 892-900
  • DOI: 10.1160/th10-08-0516
  • Peer Reviewed
• [Presentation] The both α_2-antiplasmin and plasminogen activator inhibitor type-1 gene deficient mice induces high IgE production (2014)
  • Author(s): 岡田清孝
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 新規ペプチドの皮膚創傷治癒促進作用の解析 (2014)
  • Author(s): 岡田清孝
  • Organizer: 第13回日本再生医療学会学術集
  • Place of Presentation: 京都
• [Presentation] 新規合成ペプチドによる皮膚創傷治癒促進効果の解析 (2014)
  • Author(s): 岡田清孝、児嶋耕太郎、奥本勝美、河尾直之、田村行識、矢野昌人、梶博史、松尾理.
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
• [Presentation] The mechanism of enhancement on plasminogen activation and thrombolysis by synthetic nonadecapeptide (2013)
  • Author(s): 岡田清孝
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] The mechanism of enhancement on plasminogen activation and thrombolysis by synthetic nonadecapeptide. (2013)
  • Author(s): Okada K, Ueshima S, Matsuno H, Kawao N, Tamura Y, Yano M, Nagai N, Matsuo O
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀 (東京)
• [Presentation] 特別講演「創傷治癒過程における細胞性線溶を中心とした蛋白分解カスケードの機能」 (2012)
  • Author(s): 岡田清孝
  • Organizer: 第16回眼創傷治癒研究会
  • Place of Presentation: 奈良
• [Presentation] 肝細胞周囲での線溶系酵素/受容体系による肝再生制御機構 (2012)
  • Author(s): 岡田清孝
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 新規合成ペプチドとプラスミノーゲンの相互作用 (2012)
  • Author(s): 岡田清孝
  • Organizer: 第34回日本血栓止血学会学術集会
  • Place of Presentation: 東京
• [Presentation] 特別講演「細胞性線溶と創傷治癒」 (2011)
  • Author(s): 岡田清孝
  • Organizer: 第14回近畿眼科先進医療研究会
  • Place of Presentation: 大阪
  • Year and Date: 2011-06-08
• [Presentation] A new synthetic peptide (SP) enhances plasminogen activation by t-PA (2011)
  • Author(s): Okada K
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Kyoto
• [Presentation] A new synthetic peptide (SP) enhances plasminogen activation by t-PA. (2011)
  • Author(s): Okada K.
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis.
  • Place of Presentation: Kyoto
• [Presentation] 細胞性線溶と創傷治癒 (2011)
  • Author(s): 岡田清孝
  • Organizer: 第１４回近畿眼科先進医療研究会(招待講演)
  • Place of Presentation: 大阪