| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
G protein coupled receptors (GPCRs) are the important signaling molecules which are activated upon the binding with the specific ligand and trigger the downstream signaling through activating G protein. These initial processes of GPCR signaling, the activation of receptor and the following G protein binding, were analyzed by using a fluorescence resonance energy transfer (FRET) technique. With the high time resolution FRET analysis, we found that binding of G protein stabilizes the active conformations of GPCRs and slows the deactivation of the activated receptor. Further FRET studies revealed that the effects of G protein binding differ depending on the type of the coupled receptors.
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