| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Growth cone collapse is a crucial process for repulsive axon guidance and is accompanied by a reduction in growth cone surface area. This process of reduction may be regulated by endocytosis; however, its molecular mechanism is unclear. Macropinocytosis is a clathrin-independent form of endocytosis in which large areas of plasma membrane can be engulfed. We examined the effects of macropinocytosis inhibitor, ethylisopropylamiloride (EIPA) on Sema3A-induced growth cone collapse in chicken dorsal root ganglion neurons. We found that EIPA suppressed Sema3A-induced macropinocytosis (uptake of high-molecular weight dextran) and growth cone collapse, as shown in Figure 1. These results indicate that macropinocytosis-mediated massive internalization of the plasma membrane is required for Sema3A-induced growth cone collapse.
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