| Project/Area Number |
23590278
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
|
|---|
| Research Institution | Shimane University |
|---|
Principal Investigator |
SHIDO Osamu 島根大学, 医学部, 教授 (40175386)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUZAKI Kentaro 島根大学, 医学部, 助教 (90457185)
KATAKURA Masanori 島根大学, 医学部, 助教 (40383179)
HASHIMOTO Michio 島根大学, 医学部, 准教授 (70112133)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 暑熱曝露 / 暑熱馴化 / アクアポリン / 唾液腺 / 口腔乾燥症 / 運動トレーニング / ＨＳＰ / HIF-1α / ドライマウス / 培養細胞 |
|---|
| Research Abstract |
We investigated mechanism of heat exposure-induced improvement of salivary function in rats. Two-day heat exposure increased expressions of aquaporin (AQP)1 and 5 mRNA in submandibular glands of rats concomitant with the promotion of the HIF-1a pathway, leading to VEGF induction. The protein level of AQP1 was raised after 2 or 5 day-heat exposure. The apical membrane distribution of AQP5 in serous acinar cells enhanced after heat acclimation, while AQP1 expression was restricted to the endothelial cells in the submandibular glands. Spontaneous exercise for 40 days tended to elevate AQP1 protein level of the rat submandibular glands, whereas it failed to affect AQP5 expression and protein levels of TRPV1, V3 or Na-K ATPase, relating to induce AQP5. Pilocarpine-induced salibary secretion was slightly elevated. These results suggest that in rats, heat exposure or exercise facilitates AQP expression in salivary glands, which may then contribute to improving saliva gland function.
|
