| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We have shown earlier that during development, somatostatin (sst) gene is transiently transcribed in neurons in the sexually dimorphic nucleus of the rat preoptic area (SDN-POA) characterized by calbindin immunoreactivity. We hypothesized that mechanisms other than apoptosis are involved in the establishment of the SDN-POA. BrdU immunoreactivity showed up in calbindin-labeled neurons in the SDN-POA of PD15 cohorts treated on 14, 16 or 18 embryonic days (ED). The number of BrdU-positive neurons was largest in animals given BrdU on 18 ED. Daily injections of BrdU during postnatal day 1-10 labeled a few calbindin-immunoreactive cells in the SDN-POA of PD15 brain of male and female rats. Next step, we analyzed the physiological function of the male rat SDN-POA by the treatments of sst-siRNA recombinant adenovirus vector and esi sst-siRNA followed by preference and sexual behavior test. Sst neurons expressed in the SDN-POA are unlikely involved in sexual behavior of male rat.
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