Exploring the causes of arteriosclerosis from the expressed microRNA by the dedifferentiation of vascular smooth muscle cells.
Project/Area Number |
23590295
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General pharmacology
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Research Institution | Gunma University |
Principal Investigator |
NAKAMURA Akio 群馬大学, 医学(系)研究科(研究院), 講師 (30282388)
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 血管平滑筋細胞 / 形質転換 / マイクロRNA / 動脈硬化性疾患 / マイクロRNA解析 / リン酸化プロテオーム解析 / エキソソーム / 収縮・弛緩 / 細胞増殖 / 細胞遊走 / ニコチン / 血管平滑筋 / エキソーム / 脱分化 / プロテオミクス / 動脈硬化症 |
Outline of Final Research Achievements |
We indicated that the exposure of nicotine had changed the human vascular smooth muscle cells (HSMCs) phenotype from contractile-type to a synthetic-like type by the results of the exhaustive gene expression profiling analysis. When the HSMCs were de-differentiated into synthetic phenotype, their migration increases, which might be the result of enhanced activation of MAPKs. We propose that nicotine directly may promote the migration of HSMCs from the tunica media to atheromatous plaques in the vascular intima. We have identified a microRNA that varies in accordance with the de-differentiation of the HSMCs.
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Report
(5 results)
Research Products
(57 results)
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[Journal Article] Nicotine exposure alters human vascular smooth muscle cell phenotype from a contractile to a synthetic type.2014
Author(s)
Yoshiyama, S., Chen, Z., Okagaki, T., Kohama, K., Nasu- Kawaharada. R., Izumi, T., Ohshima, N., Nagai, T., and Nakamura, A.
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Journal Title
Atherosclerosis
Volume: 237(2)
Issue: 2
Pages: 464-470
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Interaction of peptide-bound beads with lipopolysaccharide and lipoproteins.2014
Author(s)
Suzuki,M.M.,Matsumoto,M.,Omi,H.,Kobayashi,T.,Nakamura, A.,Kishi,H.,Kobayashi, S., and Takagi, T.
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Journal Title
J Microbiol Methods.
Volume: 100
Pages: 137-141
DOI
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Peer Reviewed
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[Journal Article] Tunicate cytostatic factor TC14-3 induces a polycomb group gene and histone modification through Ca2+ binding and protein dimerization2012
Author(s)
Kawamura, K., Takakura, K., Mori, D., Ikeda, K., Nakamura, A., Suzuki, T.
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Journal Title
BMC Cell.Biol.
Volume: 13
Issue: 1
Pages: 1-13
DOI
Related Report
Peer Reviewed
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[Journal Article] Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.2011
Author(s)
Thatcher SE, Fultz ME, Tanaka H, Hagiwara H, Zhang HL, Zhang Y, Hayakawa K, Yoshiyama S, Nakamura A, Wang HH, Katayama T, Watanabe M, Lin Y, Wright GL, Kohama K.
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Journal Title
J. Pharmacol. Sci
Volume: 116
Pages: 116-127
NAID
Related Report
Peer Reviewed
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[Presentation] A Novel Physarum Phosphatase is related to transition to Sclerotium (Spherule) from plasmodium.2014
Author(s)
Okada,C., Nakamura, A.,Ogawa,K.,Kohama, K., Kaneko, T.
Organizer
15thIUBMB&24th FABMB-TSBMB
Place of Presentation
Taipei, Taiwan
Year and Date
2014-10-21 – 2014-10-26
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[Presentation] Antihypertensive effect and relaxant activity of a post-fermentation tea extract.
Author(s)
Nakamura, A., kawaharada, R., Yoshiyama, S., Takazawa, K., Shimizu, H., Masuda, H., Inoue, N. and Inoue, T.
Organizer
The 5th International Conference on 0-CHA Culture and Science
Place of Presentation
Shizuoka, Japan
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