Investigation of the role of c-Src and MAP kinases in diabetic microangiopathy and development of the new molecular pharmacotherapy
| Project/Area Number |
23590306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Kentaro 奈良県立医科大学, 医学部, 准教授 (80507393)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 糖尿病性微小血管障害 / c-Src / MAPキナーゼ / アンジオテンシンII / 血管平滑筋 / 細胞遊走 / アンジオテンシンＩＩ / ｃ－Ｓｒｃ / ＭＡＰキナーゼ |
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| Research Abstract |
We investigated the possible involvement of Src tyrosine kinase and MAP kinases indiabetic microangiopathy. Angiotensin II (AII) plays an important role in insulin resistance in the vasculature. We examined the effects of PP2, a Src tyrosine kinase inhibitor and Src siRNA for Src gene silencing on AII-induced rat aortic smooth muscle cell (RASMC) migration. We found that PP2 and Src siRNA both inhibited AII-induced ERK1/2 and JNK activation in RASMC. We also found that both PP2 and Src siRNA strongly inhibited AII-induced RASMC migration. From these findings, it was inferred that direct inhibition of Src may be an effective option for vascular smooth muscle cell growth and migration. Pharmacological or genetic inhibition of Src may be an additional strategy to renin-angiotensin blockade for AII involved insulin resistance such as diabetic microangiopathy.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] S-nitrosylation regulates mitochondrial quality control via activation of2013
Author(s)
Ozawa K , Komatsubara AT, Nishimura Y, Sawada T, Kawafune H, Tsumoto H, Tsuji Y, Zhao J, Kyotani Y, Tanaka T, Takahashi R, Yoshizumi M
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Journal Title
Scientific Reports
Volume: 3: 2202
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Intermittent hypoxia induces the proliferation of rat vascular smooth muscle cell with the increases in epidermal growth factor family and erbB2 receptor.2013
Author(s)
Yoji Kyotani, Hiroyo Ota, Asako Itaya-Hironaka, Akiyo Yamauchi, Sumiyo Sakuramoto-Tsuchida, Jing Zhao, Kentaro Ozawa, Kosuke Nagayama, Satoyasu Ito, Shin Takasawa, Hiroshi Kimura, Masayuki Uno, Masanori Yoshizumi
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Journal Title
Exp. Cell Res.
Volume: 319
Issue: 19
Pages: 3042-3050
DOI
Related Report
Peer Reviewed
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[Presentation] Vascular Smooth Muscle Cell Proliferation by Intermittent Hypoxia in Indipendent on the Glucose- Induced insulin Secretion2012
Author(s)
Yoji Kyotani, Hiroko Ota, Asako Itaya- Hironaka, Akiyo Yamauchi, Sumiyo Sakuramoto-Tsuchida, Jing Zhao, Hiroshi Kimura, Masayuki Uno, Shin Takasawa, Masanori Yoshizumi
Organizer
72nd Scientific Sessions, American Diabetes Association
Place of Presentation
Philadelphia, PA
Related Report
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[Presentation] Vascular Smooth Muscle Cell Proliferation by Intermittent Hypoxia in Indipendent on the Glucose-Induced insulin Secretion2012
Author(s)
Yoji Kyotani, Hiroko Ota, Asako Itaya-Hironaka, Akiyo Yamauchi, Sumiyo Sakuramoto-Tsuchida, Jing Zhao, Hiroshi Kimura, Masayuki Uno, Shin Takasawa, Masanori Yoshizumi
Organizer
72nd Scientific Sessions, American Diabetes Association
Place of Presentation
Philadelphia, PA, USA
Related Report
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