| Project/Area Number |
23590316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Shinya H 兵庫医科大学, 医学部, 講師 (70273703)
NAGANO Takayuki 兵庫医科大学, 医学部, 助教 (10368516)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | ヒスタミン / 拘束水浸ストレス / 代謝酵素 / 生理活性物質 / ECL細胞 / ヒスチジン脱炭酸酵素 |
|---|
| Research Abstract |
Restrained water immersion stress of 30 min (WRS) in mice and rats caused mild ulcer in the gastric mucosa. Histamine content in the gastric mucosa increased 1.6 and 1.3 times, respectively. Anti-histamine N-methyltransferase (HMT) antibody detected a band in rat kidney, but not in gastric mucosa. Quantitative PCR detected HMT mRNA in gastric mucosa, but the expression level did not change by WRS. Anti-histidine decarboxylase antibody detected a single band in the rat gastric mucosa but the content did not change by WRS. Anti-diamine oxidase antibody failed to detect expression in the gastric mucosa. The phosphorylation level of MAP kinases (ERK1/2, SAPK/KNK, p38) did not change by WRS.
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