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Functional analyses of large Maf transcription factors in the development of pancreatic endocrine cells and its application

Research Project

Project/Area Number 23590325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

OISHI Hisashi  筑波大学, 医学医療系, 准教授 (30375513)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Satoru  筑波大学, 医学医療系, 教授 (50271896)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsインシュリン / β細胞 / 大Maf群転写因子 / マウス / 糖尿病 / インスリン / 生物発光
Research Abstract

It is an important research subject to understand the mechanisms of pancreatic beta-cell development and maturation for the achievement of regeneration therapy for diabetes. The project yielded these three results: (1) To analyze the function of large Maf transcription factors in pancreatic endocrine cells, we generated floxed MafB mice. (2) To monitor the newly induced beta-like cells in mouse liver, we established the in vivo imaging system capable to quantify the insulin gene activity (3) Dominant role of MafA in the beta-like cell conversion in comparison with MafB.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (16 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Bioluminescence imaging of β cells and intrahepatic insulin gene activity under normal and pathological conditions2014

    • Author(s)
      Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Tai PH, Ema M, Kudo T, Takahashi S
    • Journal Title

      Plos One

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo monitoring of pancreatic β -cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging2013

    • Author(s)
      Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S
    • Journal Title

      Plos One

      Volume: 8 Issue: 4 Pages: e60411-e60411

    • DOI

      10.1371/journal.pone.0060411

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A bacterial artificial chromosome transgene with polymorphic Cd72 inhibits the development of glomerulonephritis and vasculitis in MRL-Faslpr lupus mice2013

    • Author(s)
      Oishi, H., Tsubaki, T., Miyazaki, T., Ono, M., Nose, M. and Tahahashi, S.
    • Journal Title

      Journal of Immunology

      Volume: 190 Issue: 5 Pages: 2129-2137

    • DOI

      10.4049/jimmunol.1202196

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice.2012

    • Author(s)
      Oishi H et al.
    • Journal Title

      J. Biochem

      Volume: 152 Issue: 6 Pages: 549-555

    • DOI

      10.1093/jb/mvs100

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sekiguchi Y, Owada J, Oishi H, Katsumata T, Ikeda K, Kudo T, Takahashi S2012

    • Author(s)
      Noninvasive monitoring ofβ-cell mass and fetalβ-cell genesis in mice using bioluminescence imaging
    • Journal Title

      Exp Anim

      Volume: 61(4) Pages: 445-451

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Colmegna I, Pryshchep S, Oishi H, Goronzy JJ, Weyand CM2012

    • Author(s)
      Dampened ERK signaling in hematopoietic progenitor cells in rheumatoid arthritis
    • Journal Title

      Clin Immunol

      Volume: 143(1) Issue: 1 Pages: 73-82

    • DOI

      10.1016/j.clim.2012.01.007

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The E3 ubiquitin ligase activity of Trip12 is essential for mouse embryogenesis2011

    • Author(s)
      Kajiro M, Tsuchiya M, Kawabe Y, Furumai R, Iwasaki N, Hayashi Y, Katano M, Nakajima Y, Goto N, Watanabe T, Murayama A, Oishi H, Ema M, Takahashi S, Kishimoto H, Yanagisawa J
    • Journal Title

      PLoS One

      Volume: 6(10) Issue: 10 Pages: e25871-e25871

    • DOI

      10.1371/journal.pone.0025871

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 生物発光イメージングを用いた膵β細胞およ肝内インスリン遺伝子発現の可視化2014

    • Author(s)
      大石久史, 勝又斗紀夫, Tai Peihan, Jung Yunshin, 関口有佳里, 高橋智
    • Organizer
      第119回日本解剖学会総会・全国学術大会
    • Place of Presentation
      自治医科大学キャンパス, 栃木県下野市
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 生物発光イメージングを用いた膵β細胞および肝内インスリン遺伝子発現の可視化2014

    • Author(s)
      大石久史、勝又斗紀夫、Tai Peihan、Jung Yunshin、関口有佳里、高橋智
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学キャンパス(栃木県下野市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生物発光イメージングを用いた膵β細胞およ肝内インスリン遺伝子発現の可視化2013

    • Author(s)
      大石久史, 勝又斗紀夫, 関口有佳里, Daasi D,依馬正次, 工藤崇, 高橋智
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場, 茨城県つくば市
    • Year and Date
      2013-05-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 膵内分泌細胞発生・分化における大Maf群転写因子の役割とその応用2013

    • Author(s)
      大石久史, 工藤崇, 高橋智
    • Organizer
      第118回日本解剖学会総会・全国学術大会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場, 香川県高松市
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 生物発光イメージングを用いた膵β細胞および肝内インスリン遺伝子発現の可視化2013

    • Author(s)
      大石久史、勝又斗紀夫、関口有佳里、Dhouha Daassi、依馬正次、高橋智
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Direct Conversion of Mouse Liver Cells to Insulin-producing Cells by Defined Factors2013

    • Author(s)
      Takahiro Itagaki, Hisashi Oishi, Yutaro Itoh, Pei-Han Tai, Masami Ojima, Satoru Takahashi
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 膵内分泌細胞発生・分化における大Maf群転写因子の役割とその応用2013

    • Author(s)
      大石 久史、工藤 崇、高橋 智
    • Organizer
      第118回日本解剖学会総会・全国学術大会
    • Place of Presentation
      香川県高松市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] In vivo monitoring of pancreaticbe-cell mass and intrahepatic insulin gene activity in ins1-luc BAC transgenic mice by bioluminescence imaging2012

    • Author(s)
      Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場, 福岡県福岡市
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Bioluminescence monitoring of intrahepatic beta-like cells show distinct roles for transcription factors, MafA and MafB on the generation of insulin-producing/secreting cells in vivo2012

    • Author(s)
      Nagasaki H, Oishi H, Katsumata T, Sekiguchi Y, Kudo T, Takahashi S
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場, 福岡県福岡市
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter under the control of the insulin locus2012

    • Author(s)
      Oishi H, Katsumata T, Sekiguchi Y, Nagasaki H, Ema M, Takahashi S
    • Organizer
      第72回米国糖尿病学会
    • Place of Presentation
      フィラデルフィアコンベンションセンター, 米国フィラデルフィア
    • Year and Date
      2012-06-10
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo monitoring of intrahepatic insulin gene activity by bioluminescence imaging2011

    • Author(s)
      Sekiguchi Y, Oishi H, Katsumata T, Kudo T, Takahashi S
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜, 神奈川県横浜市
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter gene under the control of the insulin locus2011

    • Author(s)
      Katsumata T, Oishi H, Sekiguchi Y, Ema M, Kudo T, Takahashi S
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜, 神奈川県横浜市
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of Large Maf transcription factors in pancreatic endocrine cell development2011

    • Author(s)
      Oishi H, Ogata K, Kudo T, Takahashi S
    • Organizer
      3rd Beta Cell Workshop
    • Place of Presentation
      LO-Skolen, デンマーク
    • Year and Date
      2011-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] In vivo monitoring of intrahepatic insulin gene activity by bioluminescence imaging2011

    • Author(s)
      Yukari Sekiguchi, Hisashi Oishi, Tokio Katsumata, Takashi Kudo, Satoru Takahashi
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Bioluminescence imaging of novel BAC transgenic mice expressing luciferase reporter gene under the control of the insulin locus2011

    • Author(s)
      Tokio Katsumata, Hisashi Oishi, Yukari Sekiguchi, Masatsugu Ema, Takashi Kudo, Satoru Takahashi
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Role of Large Maf transcription factors in pancreatic endocrine cell development2011

    • Author(s)
      Hisashi Oishi, Kiyohito Ogata, Takashi Kudo, Satoru Takahashi
    • Organizer
      Beta cell workshop
    • Place of Presentation
      LO-Skolen(デンマーク)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2023-03-16  

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