Analysis of the molecular mechanism of Sertoli cell differentiation
| Project/Area Number |
23590329
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Asahikawa Medical College (2012-2013)
University of Fukui (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | セルトリ細胞 / SF-1/Ad4BP / 性分化 / SOX9 / ステロイドホルモン |
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| Research Abstract |
Testicular Sertoli cells support spermatogenesis by secreting various cytokines and growth factors in adult,whereas they are essential for sex determination and differentiation in fetus. In this study, we tried to establish the systems to induce the differentiation of stem or progenitor cells into mature Sertoli cells,and to investigate the molecular mechanisms of Sertoli cell differentiation.Progenitor cells differentiated into the mature Sertoli cell-like cells by introducing SF-1/Ad4BP and other two transcription factors. Using this system, it was also demonstrated that transcriptional coactivator,peroxisome proliferator activating receptor coactivator-1alpha play an important role in the differentiation of the Sertoli cells.
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Report
(4 results)
Research Products
(122 results)
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[Journal Article] Transcriptional regulation of human ferredoxin reductase through an intronic enhancer in steroidogenic cells2014
Author(s)
Imamichi, Y., Mizutani, T., Ju, Y., Matsumura, T., Kawabe, S., Kanno, M., Yazawa, T., Miyamoto, K.
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Journal Title
Biochim Biophys Acta
Volume: 1839(1)
Pages: 33-42
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[Journal Article] Androgen/androgen receptor pathway regulates expression of the genes for cyclooxygenase-2 and amphiregulin in periovulatory granulosa cells2013
Author(s)
Yazawa, T., Kawabe, S., Kanno, M., Mizutani, T., Imamichi, Y., Ju, Y., Matsumura, T., Yamazaki, Y., Usami, Y., Kuribayashi, M., Shimada, M., Kitano, T., Umezawa, A., Miyamoto, K.
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Journal Title
Mol Cell Endo
Volume: 369(1-2)(IF 4.192)
Pages: 42-51
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[Journal Article] A novel isoform of liver receptor homolog-1 is regulated by steroidogenic factor-1 and the specificity protein family in ovarian granulosa cells2013
Author(s)
Kawabe, S., Yazawa, T., Kanno, M., Usami, Y., Mizutani, T., Imamichi, Y., Ju, Y., Matsumura, T., Orisaka, M., Miyamoto, K.
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Journal Title
Endocrinology
Volume: 154(4)(IF 4.459)
Pages: 1648-1660
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[Journal Article] Human glutathione S-transferase A (GSTA) family genes are regulated by steroidogenic factor 1 (SF-1) and are involved in steroidogenesis2013
Author(s)
Matsumura, T., Imamichi, Y., Mizutani, T., Ju, Y., Yazawa, T., Kawabe, S., Kanno, M., Ayabe, T., Katsumata, N., Fukami, M., Inatani, M., Akagi, Y., Umezawa, A., Ogata, T., Miyamoto, K.
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Journal Title
FASEB J
Volume: 27(8)
Pages: 3198-3208
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[Journal Article] Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells2013
Author(s)
Imamichi, Y., Mizutani, T., Ju, Y., Matsumura, T., Kawabe, S., Kanno, M., Yazawa, T., Miyamoto, K.
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Journal Title
Mol Cell Endo
Volume: 370(1-2)
Pages: 1-10
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[Journal Article] Human glutathione S-transferase A (GSTA) family genes are regulated by steroidogenic factor 1 (SF-1) and are involved in steroidogenesis.2013
Author(s)
Matsumura T, Imamichi Y, Mizutani T, Ju Y, Yazawa T, Kawabe S, Kanno M, Ayabe T, Katsumata N, Fukami M, Inatani M, Akagi Y, Umezawa A, Ogata T, Miyamoto K
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Journal Title
FASEB J
Volume: 27
Issue: 8
Pages: 3198-3208
DOI
Related Report
Peer Reviewed
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[Journal Article] Nuclear receptor 5A (NR5A) family regulates 5-aminolevulinic acid synthase 1 (ALAS1) gene expression in steroidogenic cells2012
Author(s)
Ju, Y., Mizutani, T., Imamichi, Y., Yazawa, T., Matsumura, T., Kawabe, S., Kanno, M., Kangawa, K., Umezawa, A., Miyamoto, K.
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Journal Title
Endocrinology
Volume: 153(11)
Pages: 5522-5534
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[Journal Article] Proxiaml promoter of the cytochrome P450 oxidoreductase gene identification of microdeletions involving the untraslated exon 1 and crucial function of Sp1 binding sites2011
Author(s)
Soneda, S., Yazawa, T., Fukami, M., Adachi, M., Mizota, M., Miyamoto, K., Ogata, T.
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Journal Title
Clin Endocrinol Metab
Volume: 96(11)
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[Journal Article] Differentiation of mesenchymal stem cells and embryonic stem cells into steroidogenic cells using steroidogenic facror-1 and liver receptor homolog-12011
Author(s)
Yazawa, T., Kawabe, S., Inaoka, Y., Okada, R., Mizutani, T., Imamichi, Y., Ju, Y., Yamazaki, Y., Usami, Y., Kuribayashi, M., Umezawa, A., Miyamoto, K.
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Journal Title
Mol Cell Endo
Volume: 336
Pages: 127-132
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[Journal Article] Stem cell differentiation into steroidogenic cells lineages by NR5A family2011
Author(s)
Miyamoto, K. Yazawa, T., Mizutani, T., Imamichi, Y., Kawabe, S., Kanno, M., Matsumura, T., Ju, Y., , Umezawa, A.
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Journal Title
Mol Cell 1Endo
Volume: 336
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[Presentation] 卵巣顆粒膜細胞におけるLRH-1のプロモーター解析2013
Author(s)
河邉真也,矢澤隆志,菅野真史,宇佐美陽子,水谷哲也,今道力敬,具云峰,松村健大,宮本薫
Organizer
第86回日本内分泌学会学術総会
Place of Presentation
仙台(日本内分泌学会雑誌89(1),247,2013)
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[Presentation] Differentiation of mesenchymal stem cells and embryonic stem cells into steroidogenic cells using SF-1 and LRH-12012
Author(s)
Yazawa, T., Kawabe, S., Kanno, M., Mizutani, T., Imamichi, Y., Ju, Y., Matsumura T., Umezawa, A., Miyamoto, K.
Organizer
15th International Congress on Hormonal Steroids and Hormones & Cancer
Place of Presentation
Kanazawa, Japan
Related Report
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[Presentation] Identification of a novel ovarian specific isoform promoter of liver receptor homolog-1 in ovarian granulosa cells2012
Author(s)
Kawabe, S., Yazawa, T., Kanno, M., Usami, Y., Mizutani, T., Imamichi, Y., Ju, Y., Matsumura, T., Miyamoto, K.
Organizer
The 94th Annual Meeting & Expo (ENDO 2012)
Place of Presentation
Houston, TEXAS.(Abstract 79, 2012)
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