• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Electrolytes gradient dependent nutritional absorption.

Research Project

Project/Area Number 23590334
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

TAMURA Atsushi  大阪大学, 生命機能研究科, 准教授 (00362525)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsタイトジャンクション / クローディン / イオンホメオスターシス / 細胞間接着装置 / リーキークローディン / ノックアウトマウス / 腸管吸収上皮 / 水の透過性 / 腸管 / 栄養吸収 / 脂肪酸 / 胆汁酸
Research Abstract

Multicellular organism is surrounded by the epithelial cell sheets. Epithelial cell sheet is composed of epithelial cells which are adhered each other by cell-cell adhesion complex. Epithelial cell sheets cover inside and outside of our body and the play a biological specific role as organ like skin, intestine and so on. In this research, I analyzed the tight junction mechanism, by which biologically adequate microenvironment can be formed in our body. In this study, I focused especially on small intestine using clausdin-2 and -15 knockout mice. The claudin-2 and -15 knockout mice showed imbalanced electrolytes of intestine induced malnutrition and infantile death.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (39 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (24 results) (of which Invited: 2 results) Remarks (6 results)

  • [Journal Article] The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK2013

    • Author(s)
      Yano T, Matsui T, Tamura A, Uji M, Tsukita S.
    • Journal Title

      J Cell Biol.

      Volume: 203 Issue: 4 Pages: 605

    • DOI

      10.1083/jcb.201304194

    • URL

      https://pure.teikyo.jp/en/publications/e47c61af-9030-4418-8a53-80b72d7f9f74

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of claudins 2 and 15 from mice causes defects in paracellular Na <sup>+</sup> flow and nutrient transport in gut and leads to death from malnutrition2013

    • Author(s)
      Wada M, Tamura A, Takahashi N, Tsukita S
    • Journal Title

      Gastroenterol.

      Volume: 144 Issue: 2 Pages: 369

    • DOI

      10.1053/j.gastro.2012.10.035

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE32013

    • Author(s)
      Hayashi H., et al
    • Journal Title

      PLoS One

      Volume: 8 Issue: 2 Pages: e55623-e55623

    • DOI

      10.1371/journal.pone.0055623

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loss of Claudins 2 and 15 From Mice Causes Defects in Paracellular Na(+) Flow and Nutrient Transport in Gut and Leads to Death from Malnutrition.2013

    • Author(s)
      Wada M., et al
    • Journal Title

      Gastroenterology

      Volume: 114 Pages: 369-380

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins2012

    • Author(s)
      Lei Z., et al
    • Journal Title

      Dev Biol.

      Volume: 371 Issue: 2 Pages: 136

    • DOI

      10.1016/j.ydbio.2012.07.005

    • URL

      https://pure.teikyo.jp/en/publications/7036e9cb-7d83-4ab2-942c-c4ab729fce84

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Claudin-based paracellular proton barrier in the stomach2012

    • Author(s)
      Tamura A., et al
    • Journal Title

      Ann N Y Acad Sci.

      Volume: 1258 Issue: 1 Pages: 108

    • DOI

      10.1111/j.1749-6632.2012.06570.x

    • URL

      https://pure.teikyo.jp/en/publications/01ea51f6-8f1c-48ec-af74-c96f40df7906

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deficiency of claudin-18 causes paracellular H<sup>+</sup> leakage, up-regulation of interleukin-1β, and atrophic gastritis in mice2012

    • Author(s)
      Hayashi, D., Tamura, A., Tanaka, H., Yamazaki, Y., Watanabe S. Suzuki, K., Suzuki, K. Sentani, K., Yasui, W., Rakugi, H., Isaka, Y., and Tsukita, S.
    • Journal Title

      Gastroenterology

      Volume: 142 Issue: 2 Pages: 292

    • DOI

      10.1053/j.gastro.2011.10.040

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet2012

    • Author(s)
      Kunimoto, K., Yamazaki, Y., Nishida, T., Shinohara, K., Ishikawa, H., Hasegawa, T., Okanoue, T., Hamada H., Noda, T., Tamura, A., Tsukita, S., and Tsukita, S.
    • Journal Title

      Cell

      Volume: 148 Issue: 1-2 Pages: 189

    • DOI

      10.1016/j.cell.2011.10.052

    • URL

      https://pure.teikyo.jp/en/publications/4bd2daf2-8b49-461b-9ed8-be38cc62ce5c

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel expression of claudin-5 in glomerular podocytes2011

    • Author(s)
      Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
    • Journal Title

      Cell Tissue Res

      Volume: 343 Issue: 3 Pages: 637

    • DOI

      10.1007/s00441-010-1117-y

    • URL

      https://pure.teikyo.jp/en/publications/a4f57803-0b52-4988-8b59-8d9975cb4f3a

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 腸管特異的Claudin-7欠損はタイトジャンクション透過性を変化させ, 大腸炎を惹起する2013

    • Author(s)
      田中啓雄, 武知真希, 田村淳, 山崎裕自, 月田早智子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2013-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 肝・胆道系の胆汁フロー調節におけるクローディンの役割2013

    • Author(s)
      今里光伸, 山崎裕自, 田中啓雄, 松本健吾, 国本晃司, 田村淳, 渡辺光博, 月田早智子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2013-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of tight junction in biological systems-more than a simple paracellular barrier in inflammation and metabolism2013

    • Author(s)
      Atsushi Tamura, and Sachiko Tsukita
    • Organizer
      IUPS
    • Place of Presentation
      Birmingham. England
    • Year and Date
      2013-07-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Biological function of paracellular barrier - forming claudins and claudin-deficiency-related inflammation2013

    • Author(s)
      田村淳, 月田早智子
    • Organizer
      日本病理学会総会
    • Place of Presentation
      札幌
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Claudin-18 is according to structure tight junction not to leakage of gastric acid in mouse stomach2013

    • Author(s)
      Koya Suzuki, Atsushi Tamura, Daisuke Hayashi, Yuji Ymamazaki,and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structual Physiology
    • Place of Presentation
      名古屋
    • Year and Date
      2013-01-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Intestine specific loss of Claudin-7 causes tight junction permeability change and intestinal inflammation2013

    • Author(s)
      Hiroo Tanaka, Maki Takechi, Atsushi Tamura,Yuji Yamazaki, and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structual Physiology
    • Place of Presentation
      名古屋
    • Year and Date
      2013-01-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Loss of Claudin-2 and 15 from Mice Causies Defects in Paracellular Na+ Flow and Nutritional Transport in Lumen and Leads to Death from Malnutrition2013

    • Author(s)
      Masami Wada, Atsushi Tamura,Nobuyuki Takahashi, and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structual Physiology
    • Place of Presentation
      名古屋
    • Year and Date
      2013-01-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 上皮細胞間接着を起としたアピカル細胞骨格構築2013

    • Author(s)
      月田早智子, 矢野智樹, 立石和博, 田村淳, 山崎裕自
    • Organizer
      2013生体運動研究合同班会議
    • Place of Presentation
      広島
    • Year and Date
      2013-01-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Biological function of paracellular barrier-forming claudins and claudin-deficiency-related inflammation2013

    • Author(s)
      田村淳、月田早智子
    • Organizer
      第102回 日本病理学会総会
    • Place of Presentation
      ロイトン札幌
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 上皮細胞間タイトジャンクションを起点と下上皮細胞シートの高次構築2012

    • Author(s)
      矢野智樹, 立石和博, 伊藤雅彦, 小倉祐輝, 田村淳, 山崎裕自, 月田早智子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] The role of claudin in regulating the liver-biliary system International Conference Merida 20122012

    • Author(s)
      Yuji Yamazaki, Mitsunobu Imasato,Hiroo Tanaka, Kengo Matsumoto, Atsushi Tamura, Koshi Kunimoto,Mitsuhiro Watanabe, Sachiko Tsukita
    • Organizer
      Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
    • Place of Presentation
      Mexico
    • Year and Date
      2012-11-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Coodinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet2012

    • Author(s)
      Koshi Kunimoto, Yuji Yamazaki, Tomoki Nishida, Kyosuke Shinohara, Hiroaki Ishikawa, Toshiaki Hasegawa, Takeshi Okanouem Hiroshi Hamada, Tetsuo Noda ,Atsushi Tamura, Shoichiro Tsukita Sachiko Tsukita
    • Organizer
      大阪バイオサイエンス研究所マンスリーレクチャー
    • Place of Presentation
      大阪
    • Year and Date
      2012-04-11
    • Related Report
      2013 Final Research Report
  • [Presentation] タイトジャンクションを起点としたアピカル表層構築の解析2012

    • Author(s)
      Tomoki Yano, Masahiko Ito, Takeshi Matsui, Atsushi Tamura, Katsushi Owaribe, Masami Uji, Yuji Yamazaki, and Sachiko Tsukita
    • Organizer
      ASCB 2012 Annual meeting
    • Place of Presentation
      モスコンセンター
    • Related Report
      2012 Research-status Report
  • [Presentation] 上皮細胞間タイトジャンクションを起点とした上皮細胞シートの高次構築2012

    • Author(s)
      矢野智樹、立石和博、伊藤雅彦、小倉祐輝、田村淳、山崎裕自、月田早智子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation]

    • Author(s)
      Tomoki Yano, Masahiko Ito, Takeshi Matsui, Atsushi Tamura, Katsushi Owaribe, Masami Uji, Yuji Yamazaki, Sachiko Tsukita
    • Organizer
      ASCB 2012 annual meeting
    • Place of Presentation
      Sanfrancisco
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of tight junction claudins in biological systems-more than a simple paracellular barrier in inflammmation and metabolism.

    • Author(s)
      Atsushi Tamura, Sachiko Tsukita
    • Organizer
      IUPS
    • Place of Presentation
      バーミンガム(イギリス)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Claudin-18 is according to structure tight junction not to leakage of gastric acid in mouse stomach.

    • Author(s)
      Koya Suzuki, Atsushi Tamura, Daisuke Hayashi, Yuji Yamazaki, and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学 豊田講堂
    • Related Report
      2012 Research-status Report
  • [Presentation] Intestine specific loss of Claudin-7 causes tight junction permeability change and intestinal inflammation.

    • Author(s)
      Hiroo Tanaka, Maki Takechi, Atsushi Tamura, Yuji Yamazaki, and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学 豊田講堂
    • Related Report
      2012 Research-status Report
  • [Presentation] Loss of Claudins 2 and 15 from Mice Causes Defects in Paracellular Na+ Flow and Nutrient Transport in Lumen and Leads to Death from Malnutrition

    • Author(s)
      Masami Wada, Atsushi Tamura, Nobuyuki Takahashi, and Sachiko Tsukita
    • Organizer
      Nagoya Symposium Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学 豊田講堂
    • Related Report
      2012 Research-status Report
  • [Presentation] 上皮細胞間接着を起点としたアピカル細胞骨格構築

    • Author(s)
      月田早智子、矢野智樹、立石和博、田村淳、山崎裕自
    • Organizer
      2013生体運動研究合同班会議
    • Place of Presentation
      広島大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 腸管特異的Cludin-7欠損はタイトジャンクション透過性を変化させ、大腸炎を惹起する

    • Author(s)
      田中啓雄、武知真希、田村淳、山崎裕自、月田早智子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 肝・胆道系の胆汁フロー調節におけるクローディンの役割

    • Author(s)
      今里光伸、山崎裕自、田中啓雄、松本健吾、国本晃司、田村淳、渡辺光博、月田早智子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] The role of claudins in regulating the liver-biliary system

    • Author(s)
      Yuji Yamazaki, Mitsunobu Imasato, Hiroo Tanaka, Kengo Matsumoto, Atsushi Tamura, Koshi Kunimoto, Mitsuhiro Watanabe, and Sachiko Tsukita
    • Organizer
      International Conference Merida 2012 Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
    • Place of Presentation
      Hyatt Regency Hotel in Merida
    • Related Report
      2012 Research-status Report
  • [Presentation] Coordinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet

    • Author(s)
      Koshi Kunimoto, Yuji Yamazaki, Tomoki Nishida, Kyosuke Shinohara, Hiroaki Ishikawa, Toshiaki Hasegawa, Takeshi Okanoue, Hiroshi Hamada, Tetsuo Noda, Atsushi Tamura, et al.
    • Organizer
      大阪バイオサイエンス研究所マンスリーレクチャー
    • Place of Presentation
      大阪バイオサイエンス研究所
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/tsukita/

    • Related Report
      2013 Final Research Report
  • [Remarks] 大阪大学 分子生体情報学 ホームページ

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/tsukita/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 大阪大学大学院生命機能研究科分子生体情報学研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/13/

    • Related Report
      2012 Research-status Report
  • [Remarks] 大阪大学大学院生命機能研究科/医学系研究科 月田研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/tsukita/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/general/lab/13/

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/tsukita/

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi