Electrolytes gradient dependent nutritional absorption.

• Project/Area Number: 23590334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: TAMURA Atsushi 大阪大学, 生命機能研究科, 准教授 (00362525)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: タイトジャンクション / クローディン / イオンホメオスターシス / 細胞間接着装置 / リーキークローディン / ノックアウトマウス / 腸管吸収上皮 / 水の透過性 / 腸管 / 栄養吸収 / 脂肪酸 / 胆汁酸

Research Abstract

Multicellular organism is surrounded by the epithelial cell sheets. Epithelial cell sheet is composed of epithelial cells which are adhered each other by cell-cell adhesion complex. Epithelial cell sheets cover inside and outside of our body and the play a biological specific role as organ like skin, intestine and so on. In this research, I analyzed the tight junction mechanism, by which biologically adequate microenvironment can be formed in our body. In this study, I focused especially on small intestine using clausdin-2 and -15 knockout mice. The claudin-2 and -15 knockout mice showed imbalanced electrolytes of intestine induced malnutrition and infantile death.

Research Products

• [Journal Article] The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK. (2013)
  • Author(s): Yano T, Matsui T, Tamura A, Uji M, Tsukita S.
  • Journal Title: J Cell Biol. Volume: 25 Issue: 4 Pages: 605-614
  • DOI: 10.1083/jcb.201304194
  • Peer Reviewed
• [Journal Article] Loss of claudins 2 and 15 from mice causes defects in paracellular Na+ flow and nutrient transport in lumen and leads to death from malnutrition (2013)
  • Author(s): Wada M, Tamura A, Takahashi N, Tsukita S
  • Journal Title: Gastroenterol. Volume: 144 Issue: 2 Pages: 369-380
  • DOI: 10.1053/j.gastro.2012.10.035
  • Peer Reviewed
• [Journal Article] Ezrin is required for the functional regulation of the epithelial sodium proton exchanger NHE3. (2013)
  • Author(s): Hayashi H., et al
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e55623-e55623
  • DOI: 10.1371/journal.pone.0055623
  • Peer Reviewed
• [Journal Article] Loss of Claudins 2 and 15 From Mice Causes Defects in Paracellular Na(+) Flow and Nutrient Transport in Gut and Leads to Death from Malnutrition. (2013)
  • Author(s): Wada M., et al
  • Journal Title: Gastroenterology Volume: 114 Pages: 369-380
  • Peer Reviewed
• [Journal Article] EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. (2012)
  • Author(s): Lei Z., et al
  • Journal Title: Dev Biol. Volume: 15 Issue: 2 Pages: 136-145
  • DOI: 10.1016/j.ydbio.2012.07.005
  • Peer Reviewed
• [Journal Article] Claudin-based paracellular proton barrier in the stomach. (2012)
  • Author(s): Tamura A., et al
  • Journal Title: Ann N Y Acad Sci. Volume: 1,258 Issue: 1 Pages: 108-114
  • DOI: 10.1111/j.1749-6632.2012.06570.x
  • Peer Reviewed
• [Journal Article] Deficiency of claudin-18 causes paracellular H+ leakage, up-regulation of interleukin-1β, and atrophic gastritis in mice. (2012)
  • Author(s): Hayashi, D., Tamura, A., Tanaka, H., Yamazaki, Y., Watanabe S. Suzuki, K., Suzuki, K. Sentani, K., Yasui, W., Rakugi, H., Isaka, Y., and Tsukita, S.
  • Journal Title: Gastroenterology Volume: 142 Issue: 2 Pages: 292-304
  • DOI: 10.1053/j.gastro.2011.10.040
  • Peer Reviewed
• [Journal Article] Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet. (2012)
  • Author(s): Kunimoto, K., Yamazaki, Y., Nishida, T., Shinohara, K., Ishikawa, H., Hasegawa, T., Okanoue, T., Hamada H., Noda, T., Tamura, A., Tsukita, S., and Tsukita, S.
  • Journal Title: Cell Volume: 148 Issue: 1-2 Pages: 189-200
  • DOI: 10.1016/j.cell.2011.10.052
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343(3) Issue: 3 Pages: 637-48
  • DOI: 10.1007/s00441-010-1117-y
  • Peer Reviewed
• [Presentation] 腸管特異的Claudin-7欠損はタイトジャンクション透過性を変化させ, 大腸炎を惹起する (2013)
  • Author(s): 田中啓雄, 武知真希, 田村淳, 山崎裕自, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-12-14
• [Presentation] 肝・胆道系の胆汁フロー調節におけるクローディンの役割 (2013)
  • Author(s): 今里光伸, 山崎裕自, 田中啓雄, 松本健吾, 国本晃司, 田村淳, 渡辺光博, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-12-14
• [Presentation] Role of tight junction in biological systems-more than a simple paracellular barrier in inflammation and metabolism (2013)
  • Author(s): Atsushi Tamura, and Sachiko Tsukita
  • Organizer: IUPS
  • Place of Presentation: Birmingham. England
  • Year and Date: 2013-07-26
• [Presentation] Biological function of paracellular barrier - forming claudins and claudin-deficiency-related inflammation (2013)
  • Author(s): 田村淳, 月田早智子
  • Organizer: 日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-06
• [Presentation] Claudin-18 is according to structure tight junction not to leakage of gastric acid in mouse stomach (2013)
  • Author(s): Koya Suzuki, Atsushi Tamura, Daisuke Hayashi, Yuji Ymamazaki,and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] Intestine specific loss of Claudin-7 causes tight junction permeability change and intestinal inflammation (2013)
  • Author(s): Hiroo Tanaka, Maki Takechi, Atsushi Tamura,Yuji Yamazaki, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] Loss of Claudin-2 and 15 from Mice Causies Defects in Paracellular Na+ Flow and Nutritional Transport in Lumen and Leads to Death from Malnutrition (2013)
  • Author(s): Masami Wada, Atsushi Tamura,Nobuyuki Takahashi, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] 上皮細胞間接着を起としたアピカル細胞骨格構築 (2013)
  • Author(s): 月田早智子, 矢野智樹, 立石和博, 田村淳, 山崎裕自
  • Organizer: 2013生体運動研究合同班会議
  • Place of Presentation: 広島
  • Year and Date: 2013-01-12
• [Presentation] Biological function of paracellular barrier-forming claudins and claudin-deficiency-related inflammation (2013)
  • Author(s): 田村淳、月田早智子
  • Organizer: 第102回 日本病理学会総会
  • Place of Presentation: ロイトン札幌
  • Invited
• [Presentation] 上皮細胞間タイトジャンクションを起点と下上皮細胞シートの高次構築 (2012)
  • Author(s): 矢野智樹, 立石和博, 伊藤雅彦, 小倉祐輝, 田村淳, 山崎裕自, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] The role of claudin in regulating the liver-biliary system International Conference Merida 2012 (2012)
  • Author(s): Yuji Yamazaki, Mitsunobu Imasato,Hiroo Tanaka, Kengo Matsumoto, Atsushi Tamura, Koshi Kunimoto,Mitsuhiro Watanabe, Sachiko Tsukita
  • Organizer: Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
  • Place of Presentation: Mexico
  • Year and Date: 2012-11-14
• [Presentation] Coodinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet (2012)
  • Author(s): Koshi Kunimoto, Yuji Yamazaki, Tomoki Nishida, Kyosuke Shinohara, Hiroaki Ishikawa, Toshiaki Hasegawa, Takeshi Okanouem Hiroshi Hamada, Tetsuo Noda ,Atsushi Tamura, Shoichiro Tsukita Sachiko Tsukita
  • Organizer: 大阪バイオサイエンス研究所マンスリーレクチャー
  • Place of Presentation: 大阪
  • Year and Date: 2012-04-11
• [Presentation] タイトジャンクションを起点としたアピカル表層構築の解析 (2012)
  • Author(s): Tomoki Yano, Masahiko Ito, Takeshi Matsui, Atsushi Tamura, Katsushi Owaribe, Masami Uji, Yuji Yamazaki, and Sachiko Tsukita
  • Organizer: ASCB 2012 Annual meeting
  • Place of Presentation: モスコンセンター
• [Presentation] 上皮細胞間タイトジャンクションを起点とした上皮細胞シートの高次構築 (2012)
  • Author(s): 矢野智樹、立石和博、伊藤雅彦、小倉祐輝、田村淳、山崎裕自、月田早智子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation]
  • Author(s): Tomoki Yano, Masahiko Ito, Takeshi Matsui, Atsushi Tamura, Katsushi Owaribe, Masami Uji, Yuji Yamazaki, Sachiko Tsukita
  • Organizer: ASCB 2012 annual meeting
  • Place of Presentation: Sanfrancisco
• [Presentation] Role of tight junction claudins in biological systems-more than a simple paracellular barrier in inflammmation and metabolism.
  • Author(s): Atsushi Tamura, Sachiko Tsukita
  • Organizer: IUPS
  • Place of Presentation: バーミンガム(イギリス)
  • Invited
• [Presentation] Claudin-18 is according to structure tight junction not to leakage of gastric acid in mouse stomach.
  • Author(s): Koya Suzuki, Atsushi Tamura, Daisuke Hayashi, Yuji Yamazaki, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: 名古屋大学 豊田講堂
• [Presentation] Intestine specific loss of Claudin-7 causes tight junction permeability change and intestinal inflammation.
  • Author(s): Hiroo Tanaka, Maki Takechi, Atsushi Tamura, Yuji Yamazaki, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: 名古屋大学 豊田講堂
• [Presentation] Loss of Claudins 2 and 15 from Mice Causes Defects in Paracellular Na+ Flow and Nutrient Transport in Lumen and Leads to Death from Malnutrition
  • Author(s): Masami Wada, Atsushi Tamura, Nobuyuki Takahashi, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: 名古屋大学 豊田講堂
• [Presentation] 上皮細胞間接着を起点としたアピカル細胞骨格構築
  • Author(s): 月田早智子、矢野智樹、立石和博、田村淳、山崎裕自
  • Organizer: 2013生体運動研究合同班会議
  • Place of Presentation: 広島大学
• [Presentation] 腸管特異的Cludin-7欠損はタイトジャンクション透過性を変化させ、大腸炎を惹起する
  • Author(s): 田中啓雄、武知真希、田村淳、山崎裕自、月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 肝・胆道系の胆汁フロー調節におけるクローディンの役割
  • Author(s): 今里光伸、山崎裕自、田中啓雄、松本健吾、国本晃司、田村淳、渡辺光博、月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] The role of claudins in regulating the liver-biliary system
  • Author(s): Yuji Yamazaki, Mitsunobu Imasato, Hiroo Tanaka, Kengo Matsumoto, Atsushi Tamura, Koshi Kunimoto, Mitsuhiro Watanabe, and Sachiko Tsukita
  • Organizer: International Conference Merida 2012 Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
  • Place of Presentation: Hyatt Regency Hotel in Merida
• [Presentation] Coordinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet
  • Author(s): Koshi Kunimoto, Yuji Yamazaki, Tomoki Nishida, Kyosuke Shinohara, Hiroaki Ishikawa, Toshiaki Hasegawa, Takeshi Okanoue, Hiroshi Hamada, Tetsuo Noda, Atsushi Tamura, et al.
  • Organizer: 大阪バイオサイエンス研究所マンスリーレクチャー
  • Place of Presentation: 大阪バイオサイエンス研究所
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/
• [Remarks] 大阪大学 分子生体情報学 ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/
• [Remarks] 大阪大学大学院生命機能研究科分子生体情報学研究室
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/13/
• [Remarks] 大阪大学大学院生命機能研究科／医学系研究科 月田研究室
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/13/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/