Epigenetic transcriptional regulation by the nuclear factor Ikappab-zeta
Project/Area Number |
23590338
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
|
Research Institution | Kyushu University |
Principal Investigator |
YAMAZAKI Soh 九州大学, 医学(系)研究科(研究院), 講師 (70315084)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 転写 / エピジェネティクス / 炎症 / 糖質コルチコイド / 抗菌タンパク質 / 発現制御 |
Research Abstract |
The transcription factor NF-kappaB is essential for expression of proinflammatory cytokines and antimicrobial proteins upon microbial infection. IkappaB-zeta is a nuclear protein required for expression of a subset of NF-kappaB-target genes. In the present study, I attempted to elucidate transcriptional regulation by IIkappaB-zeta in the chromatin context. I found that expression of two genes, both encoding antimicrobial proteins, was synergistically induced by lipopolysaccharide and glucocorticoid. I specified cis-elements responsible for the transcriptional activation by the two stimulants, and revealed that upon stimulation NF-kappaB, glucocorticoid receptor, and chromatin remodeling factors cooperatively associate with the cis-elements in an Ikappab-zeta-dependent manner.
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Report
(4 results)
Research Products
(9 results)