| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
The transcription factor NF-kappaB is essential for expression of proinflammatory cytokines and antimicrobial proteins upon microbial infection. IkappaB-zeta is a nuclear protein required for expression of a subset of NF-kappaB-target genes. In the present study, I attempted to elucidate transcriptional regulation by IIkappaB-zeta in the chromatin context. I found that expression of two genes, both encoding antimicrobial proteins, was synergistically induced by lipopolysaccharide and glucocorticoid. I specified cis-elements responsible for the transcriptional activation by the two stimulants, and revealed that upon stimulation NF-kappaB, glucocorticoid receptor, and chromatin remodeling factors cooperatively associate with the cis-elements in an Ikappab-zeta-dependent manner.
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