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Identification of novel biological functions of fetal Leydig cells

Research Project

Project/Area Number 23590339
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionKyushu University

Principal Investigator

SHIMA YUICHI  九州大学, 医学(系)研究科(研究院), 助教 (80425420)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsライディッヒ細胞 / トランスクリプトーム / 男性ホルモン / ノックアウトマウス / 核内受容体 / 分化 / ステロイドホルモン
Research Abstract

Leydig cells synthesize androgens, and thus they are essential for masculinization of the whole body. It is known that there are two type of Leydig cells, fetal leydig cells and adult Leydig cells, in the mammalian testis. However, functional differences between these two cell populations were not clarified yet. Moreover, the origin and functional importance of fetal Leydig cells were not defined at all.
In this study, we idenified that gene expression profiles are quite different between two types of Leydig cells, and they produce different types of androgens. In addition, we have identified novel functions of fetal Leydig cells by disrupting a gene specifically in the fetal Leydig cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (38 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (26 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Glycolytic gene as the targets of nuclear receptor Ad4BP/SF-12014

    • Author(s)
      Takashi Baba, Hiroyuki Otake, Tetsuya Sato, Kanako Miyabayashi, Yurina Shishido, Chia-Yih Wang, Yuichi Shima, Hiroshi Kimura, Mikako Yagi, Yasuhiro Ishihara, Shinjiro Hino, Hidesato Ogawa, Mitsuyoshi Nakao, Takeshi Yamazaki, Dongchon, Kang, Yasuyuki Ohkawa, Mikita Suyama, Bon-Chu Chung, Ken-ichirou Morohashi
    • Journal Title

      Nature Commun

      Volume: 5 Issue: 1 Pages: 3634-3634

    • DOI

      10.1038/ncomms4634

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes2013

    • Author(s)
      Yuichi Shima
    • Journal Title

      Molecular Endocrinology

      Volume: 27 Issue: 1 Pages: 63-73

    • DOI

      10.1210/me.2012-1256

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aristaless related homeobox gene Arx, is implicated in mouse fetal Leydig cell differentiation possibly through expressing in the progenitor cells2013

    • Author(s)
      Miyabayashi K, Katoh-Fukui Y, Ogawa H, Baba T, Shima Y, Sugiyama N, Kitamura K, Morohashi K
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 6 Pages: e68050-e68050

    • DOI

      10.1371/journal.pone.0068050

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 性ホルモン産生細胞の性差2013

    • Author(s)
      嶋雄一, 諸橋憲一郎
    • Journal Title

      細胞工学

      Volume: 32巻2号 Pages: 158-163

    • Related Report
      2013 Final Research Report
  • [Journal Article] 性ホルモン産生細胞の性差2013

    • Author(s)
      嶋 雄一、諸橋 憲一郎
    • Journal Title

      細胞工学

      Volume: 32 Pages: 158-163

    • Related Report
      2012 Research-status Report
  • [Journal Article] Cbx2, a polycomb group gene, is required for Sry gene expression in mice.2012

    • Author(s)
      Katoh-Fukui Y, Miyabayashi K, Komatsu T, Owaki A, Baba T., Shima, Y, Kidokoro T, Kanai Y, Schedl A, Wilhelm D, Koopman P, Okuno Y, Morohashi K.
    • Journal Title

      Endocrinology

      Volume: 153(2) Issue: 2 Pages: 913-924

    • DOI

      10.1210/en.2011-1055

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] DNA methylation of intronic enhancers directs tissue-specific expression of Steroidogenic Factor 1/Adrenal 4 Binding Protein (SF-1/Ad4BP)2011

    • Author(s)
      Erling A.Hoivik, et al
    • Journal Title

      Endocrinology

      Volume: 152 Issue: 5 Pages: 2100-2112

    • DOI

      10.1210/en.2010-1305

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] SF-1 expression during adrenal development and tumorigenesis2011

    • Author(s)
      Gardine JR, 他3名(3番目)
    • Journal Title

      Mol Cell Endocrinol

      Volume: 351 Issue: 1 Pages: 12-8

    • DOI

      10.1016/j.mce.2011.10.007

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Identification of enhancer specific for fetal Leydig cells in Ad4BP/SF-1 gene2011

    • Author(s)
      Yuichi Shima, et al
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 1 Pages: 417-425

    • DOI

      10.1210/en.2011-1407

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 胎仔型ライディッヒ細胞の細胞運命と機能2013

    • Author(s)
      嶋雄一, 宮林香奈子, 松崎佐和子, 井上実紀, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      第18回日本生殖内分泌学会学術集会
    • Place of Presentation
      東京砂防会館別館(シェーンバッハ・サボー)(口頭発表・学術奨励賞受賞)
    • Year and Date
      2013-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞の存在意義2013

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      日本アンドロロジー学会第32回学術大会
    • Place of Presentation
      大阪(特別講演)
    • Year and Date
      2013-07-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞の運命と機能2013

    • Author(s)
      嶋雄一
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「配偶子幹細胞制御機構」第7回領域会議
    • Place of Presentation
      東京海洋大学(公募研究・口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞の分化・脱分化に伴う遺伝子発現変化2013

    • Author(s)
      嶋雄一, 宮林香奈子, 松崎佐和子, 井上実紀, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「性差構築の分子基盤」第5回領域会議
    • Place of Presentation
      国民宿舎虹の松原ホテル(佐賀県唐津市)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional characterization of fetal Leydig cells in prenatal and postnatal stages2013

    • Author(s)
      Yuichi Shima, Kanako Miyabayashi, Kentaro Suzuki, Takashi Baba, Hiroyuki Otake, and Ken-ichirou Morohashi
    • Organizer
      SSR (Society for Study of Reproduction)’s 46th annual meeting
    • Place of Presentation
      Montreal, Qubec, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胎仔型ライディッヒ細胞の分化・脱分化に伴う遺伝子発現変化2013

    • Author(s)
      嶋雄一、宮林香奈子、松崎佐和子、井上実紀、馬場崇、大竹博之、諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「性差構築の分子基盤」第5回領域会議
    • Place of Presentation
      国民宿舎 虹の松原ホテル(佐賀県唐津市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胎仔型ライディッヒ細胞の細胞運命と機能2013

    • Author(s)
      嶋雄一、宮林香奈子、松崎佐和子、井上実紀、馬場崇、大竹博之、諸橋憲一郎
    • Organizer
      第18回日本生殖内分泌学会 学術集会
    • Place of Presentation
      東京砂防会館別館(シェーンバッハ・サボー)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胎仔精巣におけるテストステロン合成経路の解明2012

    • Author(s)
      嶋雄一, 宮林香奈子, 原口省吾, 馬場崇, 大竹博之, 筒井和義, 諸橋憲一郎
    • Organizer
      第85回日本生化学会大会3S02-2(シンポジウム)
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Male-specific sex differentiation regulated by fetal Leydig cells2012

    • Author(s)
      嶋雄一, 宮林香奈子, 原口省吾, 鈴木堅太郎, 馬場崇, 大竹博之, 筒井和義, 諸橋憲一郎
    • Organizer
      第35回日本分子生物学会年会1W3I-2
    • Place of Presentation
      福岡国際会議場(ワークショップ)
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Male-specific sex differentiation regulated by fetal Leydig cells2012

    • Author(s)
      嶋雄一, 宮林香奈子, 原口省吾, 鈴木堅太郎, 馬場崇, 大竹博之, 筒井和義, 諸橋憲一郎
    • Organizer
      第35回日本分子生物学会年会1P-0486
    • Place of Presentation
      マリンメッセ福岡(ポスター発表)
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔精巣におけるテストステロン合成経路の解明ライディッヒ細胞とセルトリ細胞の機能的連携2012

    • Author(s)
      嶋雄一, 宮林香奈子, 原口省吾, 馬場崇, 大竹博之, 筒井和義, 諸橋憲一郎
    • Organizer
      第17回日本生殖内分泌学会学術集会
    • Place of Presentation
      東京ステーションカンファレンス(口頭発表)
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞によるオス特異的性分化プロセスの内分泌的制御2012

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「性差構築の分子基盤」第4回領域会議
    • Place of Presentation
      和歌山マリーナシティロイヤルパインズホテル(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞によるオス特異的性分化プロセスの内分泌的制御2012

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「性差構築の分子基盤」若手研究会
    • Place of Presentation
      和歌山マリーナシティロイヤルパインズホテル(口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional Characterization of fetal Leydig cells by cell-specific disruption of Ad4BP/SF-1 gene2012

    • Author(s)
      Yuichi Shima, Kanako Miyabayashi, Takashi Baba, Hiroyuki Otake, Kentaro Suzuki, Shogo Haraguchi, Kazuyoshi Tsutsui and Ken-ichiroui Morohashi
    • Organizer
      Sixth international symposium on vertebrate sex determination
    • Place of Presentation
      Kona, Hawaii, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of fetal Leydig cell-specific Ad4BP/SF-1 gene disruption on masculinization and spermatogenesis2012

    • Author(s)
      Yuichi Shima, Kanako Miyabayashi, Takashi Baba, Hiroyuki Otake, Kentaro Suzuki, Shogo Haraguchi, Kazuyoshi Tsutsui and Ken-ichiroui Morohashi
    • Organizer
      The 58th/60th NIBB Conference, Germline –Specification, sex, and stem cells-
    • Place of Presentation
      Okazaki, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional interplay between Leydig and Serotoli cells is essential for testosterone biosynthesis in mouse fetal testis2012

    • Author(s)
      Yuichi Shima, Kanako Miyabayashi, Takashi Baba, Hiroyuki Otake, Sawako Matsuzaki, Yurina Shishido, Miki Inoue, Shogo Haraguchi, Kazuyoshi Tsutsui and Ken-ichiroui Morohashi
    • Organizer
      The 15th International Congress on Hormonal Steroids and Hormones & Cancer
    • Place of Presentation
      Kanazawa, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] 胎仔精巣におけるテストステロン合成経路の解明 ―ライディッヒ細胞とセルトリ細胞の機能的連携―2012

    • Author(s)
      嶋雄一、宮林香奈子、原口省吾、馬場崇、大竹博之、筒井和義、諸橋憲一郎
    • Organizer
      第17回日本生殖内分泌学会 学術集会
    • Place of Presentation
      東京ステーションカンファレンス
    • Related Report
      2012 Research-status Report
  • [Presentation] Male-specific sex differentiation regulated by fetal Leydig cells2012

    • Author(s)
      嶋雄一、宮林香奈子、原口省吾、鈴木堅太郎、馬場崇、大竹博之、筒井和義、諸橋憲一郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 胎仔精巣におけるテストステロン合成経路の解明2012

    • Author(s)
      嶋雄一、宮林香奈子、原口省吾、馬場崇、大竹博之、筒井和義、諸橋憲一郎
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Functional characterization of fetal Leydig cells by cell-specific disruption of Ad4BP/SF-1 gene2011

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 鈴木堅太郎
    • Organizer
      第34回日本分子生物学会年会1T5a再生・幹細胞・分化
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional characterization of fetal Leydig cells by cell-specific disruption of Ad4BP/SF-1 gene2011

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 鈴木堅太郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (1P-0223)
    • Year and Date
      2011-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞特異的Ad4BP/SF-1遺伝子ノックアウトマウスの解析2011

    • Author(s)
      嶋雄一, 馬場崇, 宮林香奈子, 大竹博之, 諸橋憲一郎
    • Organizer
      第19回日本ステロイドホルモン学会学術集会
    • Place of Presentation
      福岡大学病院メディカルホール
    • Year and Date
      2011-11-26
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 胎仔型ライディッヒ細胞の生理学的機能の解明2011

    • Author(s)
      嶋雄一, 諸橋憲一郎
    • Organizer
      第84回日本内分泌学会学術集会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2011-04-21
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 胎仔型ライディッヒ細胞特異的Ad4BP/SF-1ノックアウトマウスの解析2011

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「性差構築の分子基盤」第3回領域会議
    • Place of Presentation
      国民宿舎虹の松原ホテル(佐賀県)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] 胎仔型ライディッヒ細胞による精子形成幹細胞ニッチ形成機構の解明2011

    • Author(s)
      嶋雄一, 宮林香奈子, 馬場崇, 大竹博之, 諸橋憲一郎
    • Organizer
      文部科学省科学研究費補助金新学術領域研究「配偶子幹細胞制御機構」第5回領域会議
    • Place of Presentation
      浜名湖かんざんじ荘(静岡県)(公募研究・口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional characterization of fetal Leydig cells by cell-specific disruption of Ad4BP/SF-1 gene2011

    • Author(s)
      嶋雄一、宮林香奈子、馬場崇、大竹博之、鈴木堅太郎、山田源、諸橋憲一郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.med.kyushu-u.ac.jp/seisaseibutu/

    • Related Report
      2013 Final Research Report
  • [Remarks] 九州大学大学院 医学研究院 分子生命科学系部門 性差生物学講座

    • URL

      http://www.med.kyushu-u.ac.jp/seisaseibutu/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.med.kyushu-u.ac.jp/seisaseibutu/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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