| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The aim of this study is to investigate the molecular mechanism in the process of hematopoietic stem cells (HSCs) emergence from the hemogenic endothelium by the hematopoietic transcription factor AML1/Runx1. We originally succeeded in the production of knock in mouse which had reporter gene (GFP) targeting the Runx1 locus and could observe GFP signal in this mouse. We also discovered Runx1 depending factor, endothelial mesenchymal transition related transcription factor, by using comprehensive gene expression analysis against embryonic stem cell differentiation model. Runx1 gene knock down effects on the downregulation of this transcriptional factor and upregulation of the tight junction which dissolves at the HSCs emergence. These findings suggest that Runx1 can transform vascular endothelium with polarity and tight adhesion into HSCs via hemogenic endothelium.
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