The role of BRCA1-HERC2 complex in DNA replication fork progression and stress response
Project/Area Number |
23590348
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
|
Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
WU WENWEN 聖マリアンナ医科大学, 医学部, 助教 (10434408)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | HERC2 / BRCA1 / Claspin / 複製フォーク / ATRIP / MCM2 / 細胞周期チェックポイント / ATRIP / 細胞周期 / claspin |
Research Abstract |
HERC2 is an E3 ubiquitin ligase that targets breast cancer suppressor BRCA1 for degradation. Here we show that HERC2 is a component of the DNA replication fork complex that plays a critical role in DNA elongation and origin firing. In the presence of BRCA1, HERC2 interacts with Claspin, a protein essential for G2/M checkpoint activation and replication fork stability. Claspin depletion slowed S-phase rogression and additional HERC2 depletion reduced the effect of Claspin depletion. HERC2 interacts with replication fork complex proteins. Depletion of HERC2 alleviated the slow replication fork progression in Claspin-deficient cells, suppressed enhanced origin firing. During replication stress response, the amount of HERC2 is increased in the ATRIP complex, to result in an increase of MCM2 phosphorylation by ATR. HERC2, Claspin, and BRCA1 possibly cooperate on activation of Chk1 and Plk1 to regulate DNA replication progression and origin firing by facilitating MCM2 phosphrylation.
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Report
(4 results)
Research Products
(12 results)