Research Project
Grant-in-Aid for Scientific Research (C)
One of the biological functions of polyADP-ribosylation has been known to be involved in DNA base excision repair. However the other functions are still not known yet. To clarify the functions through identifying the in vivo acceptor proteins of polyADP-ribosylation that is mostly unknown, we used Drosophila melanogaster mutant that had deleted poly(ADP-ribose) degradation enzyme gene, (poly(ADP-ribose) glycohydrolase gene. This mutant Drosophila could eclose under elevated temperature and shows neurological disease. Thus this might serve as a model for human neurological diseases. Using affinity column of immobilized antibody against poly(ADP-ribose), alkaline treatment, 2D gel electrophoresis and mass spectroscopy, we identified alcohol dehydrogenase and several other proteins as candidates of polyADP-ribosylation in vivo.
All 2014 2013 2012 2011 Other
All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (40 results)
J Hepatobiliary Pancreat Sci
Volume: 21 Issue: 6 Pages: 397-398
10.1002/jhbp.69
Jap J Clin Oncol
Volume: 43 Issue: 12 Pages: 1190-1194
10.1093/jjco/hyt138
J Biochem.
Volume: 152(1) Issue: 1 Pages: 73-85
10.1093/jb/mvs034
10031166116
Cancer Sci
Volume: 103(2) Issue: 2 Pages: 191-196
10.1111/j.1349-7006.2011.02152.x
Oncogene
Volume: (印刷中) Issue: 23 Pages: 2849-2861
10.1038/onc.2011.462
Methods Mol.Biol.
Volume: 780 Pages: 377-390
10.1007/978-1-61779-270-0_22
Woodhead Publishing, Elsevier, Cambridge, UK(Eds. Palmiro Poltronieri and Yiguo Hong (Invited article))
Volume: (In press)
