Identification of acceptor proteins and their modification sites of polyADP-ribosylation and biological function
Project/Area Number |
23590350
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
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Research Institution | Nagahama Institute of Bio-Science and Technology |
Principal Investigator |
MIWA Masanao 長浜バイオ大学, バイオサイエンス学部, 教授 (20012750)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 翻訳後修飾 / ポリADP-リボシル化 / ショウジョウバエ変異体 / アクセプタータンパク質 / エピジェネティクス / アクセプター蛋白質 / ショウジョウバエ / 分解酵素 / 神経変性 |
Research Abstract |
One of the biological functions of polyADP-ribosylation has been known to be involved in DNA base excision repair. However the other functions are still not known yet. To clarify the functions through identifying the in vivo acceptor proteins of polyADP-ribosylation that is mostly unknown, we used Drosophila melanogaster mutant that had deleted poly(ADP-ribose) degradation enzyme gene, (poly(ADP-ribose) glycohydrolase gene. This mutant Drosophila could eclose under elevated temperature and shows neurological disease. Thus this might serve as a model for human neurological diseases. Using affinity column of immobilized antibody against poly(ADP-ribose), alkaline treatment, 2D gel electrophoresis and mass spectroscopy, we identified alcohol dehydrogenase and several other proteins as candidates of polyADP-ribosylation in vivo.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] A novel pathway of centrosome amplification that does not require DNA lesions2012
Author(s)
Tsuda, M., Tanaka, M., Mushiake, M., Takahashi, J., Tanaka, K., Watase, J., Fujisawa, J.-I., Miwa, M.
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Journal Title
Cancer Sci
Volume: 103(2)
Issue: 2
Pages: 191-196
DOI
Related Report
Peer Reviewed
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[Journal Article] Approaches to separate and identify polyADP-ribosylated proteins using poly (ADP-ribose) glycohydrolase-knockout Drosophila2011
Author(s)
Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Hasegawa, M., Komiya, T., Kondo, M., Che, F.S., Hanai, S., Miwa, M.
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Journal Title
Methods Mol.Biol.
Volume: 780
Pages: 377-390
DOI
ISBN
9781617792694, 9781617792700
Related Report
Peer Reviewed
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[Presentation] Regulation of in vivo level of polyADP-ribosylation, cell growth and centrosome amplification2014
Author(s)
Tanaka, M., Ida, C., Takahashi, J., Eguchi, T., Ohta, N., Yamashita, S., Tsukada, M., Mushiake, M., Fujii, T., Ikegami, S., Nishi, Y., Fujisawa, J., Miwa, M.
Organizer
Cold Spring Harbor Laboratory Meeting on The PARP Family & Friends : Gene regulation & beyond
Place of Presentation
Cold Spring Harbor Laboratory, New York
Related Report
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[Presentation] Detection of DNA strand Breaks with ELISA for poly(ADP-ribose)2013
Author(s)
Tsukada, M., Eguchi, T., Yamashita, S., Takahashi, J., Tanaka, M., Fujii, T., Ikegami, S., Miwa, M.
Organizer
PARP2013 19^<th> International Conference on ADP-ribosylation
Place of Presentation
Centre de Recherche du Centre Hospitalier Universitaire de Quebec-Pavillon CHUL, Quebec city, Canada
Related Report
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[Presentation] Effect of inhibition of polyADP-ribosylation on cell proliferation in CHO-K1 cells2013
Author(s)
Tanaka, M., Takahashi, J., Eguchi, T., Tsukada, M., Mushiake, M., Fujisawa, J-I., Miwa, M.
Organizer
PARP2013 19^<th> International Conference on ADP-ribosylation
Place of Presentation
Centre de Recherche du Centre Hospitalier Universitaire de Quebec-Pavillon CHUL, Quebec city, Canada
Related Report
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[Presentation] Preparation of quantities of recombinant human poly(ADP-ribose) polymerase 1 for structural analysis2011
Author(s)
Koyama, K., Eguchi, T., Morita, H., Sakai, T., Kamemura, K., Nishi, Y., Miwa, M.
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜(2P-0310)
Related Report
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[Presentation] Change of the amount of poly(ADP-ribose) in cells under physiological Condition and DNA damage2011
Author(s)
Eguchi, T., Takahashi, J., Kida, T., Fujii, T., Ikegami, S., Miwa, M.
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Related Report
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[Presentation] Verification of an acceptor protein for polyADP-ribosylation from poly(ADP-ribose) glycohydrolase-knockout Drosophila melanogaster2011
Author(s)
Yamashita, S., Eguchi, T., Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Komiya, T., Hanai, S., Miwa, M.
Organizer
第34回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Related Report
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[Presentation] ポリ(ADP-リボース)分解酵素遺伝子欠失変異による神経細胞死を呈するショウジョウバエのポリADP-リボシル化タンパク質の同定法について2011
Author(s)
三輪正直, 野殿弘人, 浜田彰子, 黒田泰仁, 亀村和生, 長谷川慎, 小宮徹, 近藤真千子, 藤井貴弘, 蔡晃植, 花井修二
Organizer
第84回日本生化学会大会
Place of Presentation
国立京都国際会館(シンポジウム1S4a-5)
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[Presentation] General method to quantify poly(ADP-ribose) and to isolate polyADP-ribosylated proteins in vivo2011
Author(s)
Miwa, M., Eguchi, T., Nodono, H., Hamada, A., Yamashita, S., Fujii, T., Kamemura, K., Komiya, T., Hasegawa, M., Che, F-S., Ikegami, S.
Organizer
FASEB Summer Research Conference : NAD Metabolism & Signaling
Place of Presentation
Lucca, Italy
Related Report
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