| Project/Area Number |
23590357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
AMANO Mutsuki 名古屋大学, 医学(系)研究科(研究院), 准教授 (90304170)
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| Co-Investigator(Kenkyū-buntansha) |
KAIBUCHI Kozo 名古屋大学, 大学院医学系研究科, 教授 (00169377)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞内シグナル伝達 / リン酸化 / キナーゼ / プロテオミクス / Rho / シグナル伝達 / 細胞骨格 |
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| Outline of Final Research Achievements |
In this study, we focused on the analysis of protein kinases downstream of Rho family small GTPases as therapeutic targets. We developed the novel substrate screening method based on the interactome analysis. By this method, a number of candidate substrates were identified for several kinases including Rho-kinase, PKN, and aPKC. We identified tumor suppressor protein Scrib and cardiomyopathy-related gene products such as CARP and MYL2 as novel Rho-kinase substrates. We identified phosphorylation sites, and analyzed the modes of action for Scrib and CARP upon the phosphorylation by Rho-kinase. We also found that PKA phosphorylates Rho-kinase, suggesting the crosstalk between PKA and Rho-kinase.
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