Involvement of small GTPase Rho family signaling pathways in diseases.
| Project/Area Number | 23590357 |
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
AMANO Mutsuki 名古屋大学, 医学(系)研究科(研究院), 准教授 (90304170)
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| Co-Investigator(Kenkyū-buntansha) |
KAIBUCHI Kozo 名古屋大学, 大学院医学系研究科, 教授 (00169377)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed(Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost : ¥4,100,000、Indirect Cost : ¥1,230,000)
Fiscal Year 2013 : ¥1,560,000 (Direct Cost : ¥1,200,000、Indirect Cost : ¥360,000)
Fiscal Year 2012 : ¥1,560,000 (Direct Cost : ¥1,200,000、Indirect Cost : ¥360,000)
Fiscal Year 2011 : ¥2,210,000 (Direct Cost : ¥1,700,000、Indirect Cost : ¥510,000)
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| Keywords | 細胞内シグナル伝達 / リン酸化 / キナーゼ / プロテオミクス / Rho / シグナル伝達 / 細胞骨格 |
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| Outline of Final Research Achievements |
In this study, we focused on the analysis of protein kinases downstream of Rho family small GTPases as therapeutic targets. We developed the novel substrate screening method based on the interactome analysis. By this method, a number of candidate substrates were identified for several kinases including Rho-kinase, PKN, and aPKC. We identified tumor suppressor protein Scrib and cardiomyopathy-related gene products such as CARP and MYL2 as novel Rho-kinase substrates. We identified phosphorylation sites, and analyzed the modes of action for Scrib and CARP upon the phosphorylation by Rho-kinase. We also found that PKA phosphorylates Rho-kinase, suggesting the crosstalk between PKA and Rho-kinase.
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Report
(5results)
Research Products
(15results)
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[Journal Article] <i>In vivo</i> Screening for Substrates of Protein Kinase A Using a Combination of Proteomic Approaches and Pharmacological Modulation of Kinase Activity2015
Author(s)
Hamaguchi, T., Nakamuta, S., Funahashi, Y., Takano, T., Nishioka, T., Shohag, M.H., Yura, Y., Kaibuchi, K., and Amano, M.
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Journal Title
Cell Struct Funct
Volume : 4
Issue : 1
Pages : 1-12
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Preferential targeting of p39-activated Cdk5 to Rac1-induced lamellipodia.2014
Author(s)
Ito, Y., Asada, A., Kobayashi, H., Takano, T., Sharma, G., Saito, T., Ohta, Y., Amano, M., Kaibuchi, K., and Hisanaga, S.
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Journal Title
Molecular and Cellular Neurosciences
Volume : 61
Pages : 34-45
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Distinct distribution and localization of Rho-kinase in mouse epithelial, muscle and neural tissues.2012
Author(s)
Iizuka, M., Kimura, K., Wang, S., Kato, K., Amano, M., Kaibuchi, K., and Mizoguchi, A.
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Journal Title
Cell structure and function
Volume : 37
Pages : 155-175
Related Report
Peer Reviewed
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