Investigation of the molecular mechanism of osteoclast function
| Project/Area Number |
23590374
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
TOHYAMA Yumi 姫路獨協大学, 薬学部, 教授 (70362770)
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| Co-Investigator(Renkei-kenkyūsha) |
KAJI Hiroaki 姫路獨協大学, 薬学部, 講師 (10368706)
TANAKA Chisato 姫路獨協大学, 薬学部, 助手 (30461122)
MORITA Hiroyuki 姫路獨協大学, 薬学部, 助手 (90648594)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 破骨細胞 / ATP / アクチン / P2X7受容体 / Syk / F-アクチン |
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| Research Abstract |
To determine the molecular mechanism of osteoclast activation, human monocytes and human leukemic cell line, HL60 were used after differentiation into ostelclast-like cells. To analyze the role of target molecules on osteoclast function, knocked down cells of PKC-delta, KIF20A, Vimentin, or SMN, were established by lentiviral transfection containing each shRNA. Knockdown of PKC-delta or Vimentin led to decreased podosome (F-actin structure) formation. Knockdown of both KIF20A and SMN increased multinuclear cells resulting from cell to cell fusion. In addition, SMN-knockdown promoted alpha-tubulin acetylation (K40), which is essential for lysosomal trasnport, suggesting that SMN is a regulator of osteoclast activation.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Spinal Muscular Atrophy: From Gene Discovery to Clinical Trials2013
Author(s)
Nurputra DK, Lai PS, Harahap NI, Morikawa S, Yamamoto T, Nishimura N, Kubo Y, Takeuchi A, Saito T, Takeshima Y, Tohyama Y, Tay SK, Low PS, Saito K, Nishio H
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Journal Title
Ann Hum Genet.
Volume: 77
Issue: 5
Pages: 435-463
DOI
Related Report
Peer Reviewed
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[Journal Article] Oligopeptides derived from autophosphorylation sites of EGF receptor suppress EGF-stimulated responses in human lung carcinoma A549 cells.2013
Author(s)
Kuroda Y, Kato-Kogoe N, Tasaki E, Murata M, Ueda K, Abe M, Miyamoto K, Nakase I, Futaki S, Tohyama Y, Hirose M
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Journal Title
Eur J Pharmacol
Volume: 698
Issue: 1-3
Pages: 87-94
DOI
Related Report
Peer Reviewed
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