Improvement of helper-dependent adenovirus vector not only increasing gene expression but also cell/tissue specificity
| Project/Area Number |
23590377
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANEGAE Yumi 東京大学, 医科学研究所, 助教 (80251453)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Izumu 東京大学, 医科学研究所, 教授 (70158913)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 遺伝子治療 / アデノウイルスベクター / 細胞特異性 |
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| Research Abstract |
The helper-dependent adenovirus vector (HD-AdV) is attractive tool for gene therapy, specially, genetic disease, because of its low-inflammation. However, it was reported that the purpose gene expression efficiency is lower than E1-substituted AdV. In this study, we determined the useful stuffer region and demonstrated an only 0.3kb DNA region near the rightend of the virus genome played an important role for efficient gene expression. And we showed that this region did not code any protein and enhancer.
We also developed a construction method of "cell/tissue-specific HD-AdV", which contains a switch unit carrying Cre gene expressed by cell/tissue-specific promoter and a target unit bearing purpose gene expression unit regulated by Cre. We chose the TH promoter for the cell/tissue-specific promoter and GFP for purpose gene. Finally, we succeeded in the construction and detected the dopamine neuron specific expression by this vector.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] Trans-complemented hepatitis C virus particles as a versatile tool for study of virus assembly and infection2012
Author(s)
Suzuki R, Saito K, Kato T, Shirakura M, Akazawa D, Ishii K, Aizaki H, Kanegae Y, Matsuura Y, Saito I, Wakita T, Suzuki T
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Journal Title
Virology
Volume: 432
Pages: 29-38
Related Report
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[Journal Article] Trans-complemented hepatitis C virus particles as a versatile tool for study of virus assembly and infection.2012
Author(s)
Suzuki R., Saito K., Kato T., Shirakura M., Akazawa D., Ishii K., Aizaki H., Kanegae Y., Matsuura Y., Saito I., Wakita T. and Suzuki T.
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Journal Title
Virology
Volume: 432
Pages: 29-38
Related Report
Peer Reviewed
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[Book] 実験医学2014
Author(s)
近藤 小貴、裴 崢、斎藤 泉、鐘ヶ江 裕美
Total Pages
6
Publisher
羊土社
Related Report
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[Book] ウイルス2013
Author(s)
近藤 小貴、前川 文、斎藤 泉、鐘ヶ江 裕美
Total Pages
14
Publisher
日本ウイルス学会
Related Report
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