Research Project
Grant-in-Aid for Scientific Research (C)
The Fanconi anemia (FA) pathway is critically involved in DNA damage responses and DNA repair. A key protein, FANCD2, is monoubiquitinated and accumulates in chromatin in response to DNA interstrand crosslinks (ICLs), where it coordinates DNA repair through mechanisms that are still poorly understand.We have isolated UBR5 and UFD1 as candidate interacting proteins for FANCD2. Both proteins contain ubiquitin-binding domains. We have established ubr5-deficient chicken DT40 cells, and SiRNA-based ufd1-knock down human cells. Unexpectedly, ubr5-deficient or ufd1-knock down cells showed little sensitivity to ICL reagents compared to wild type cells. Laser microirradiation experiments using GFP-UBR5 or GFP-UFD1 expressing human cells showed little GFP-protein accumulation after DNA damage. These results suggested that functional relationship between FA pathway and UBR5, or UFD1 including related proteins was not strong, at least in ICL repair.
All 2014 2013 2012 2011 Other
All Journal Article (13 results) (of which Peer Reviewed: 13 results, Open Access: 2 results) Presentation (16 results) (of which Invited: 6 results) Book (1 results) Remarks (4 results)
Cell Rep.
Volume: 7 Issue: 4 Pages: 1039-1047
10.1016/j.celrep.2014.04.005
Biochimica et Biophysica Acta-Molecular Cell Research
Volume: 1843 Issue: 5 Pages: 1002-1012
10.1016/j.bbamcr.2014.01.005
Cell Report
Volume: 未定
Nucleic Acids Research
Volume: VOL. 41 Issue: 14 Pages: 6930-6941
10.1093/nar/gkt467
The EMBO Journal
Volume: 31 Issue: 17 Pages: 3524-3536
10.1038/emboj.2012.197
Mol. Cell
Pages: 47-47
Volume: 40 Issue: 10 Pages: 4553-4561
10.1093/nar/gks053
Molecular Cell
Volume: 47 Issue: 4 Pages: 511-522
10.1016/j.molcel.2012.05.047
Methods Mol Biol.
Volume: 920 Pages: 39-49
10.1007/978-1-61779-998-3_4
120004996387
Cancer Research
Volume: 72 Issue: 5 Pages: 1149-1156
10.1158/0008-5472.can-11-2904
J. Exp. Med
Volume: 208 Issue: 7 Pages: 1447-1457
10.1084/jem.20102665
Biochem.Biophys.Res.Commun.
Volume: 410 Issue: 3 Pages: 568-573
10.1016/j.bbrc.2011.06.027
PLoS Genet
Volume: 7(8) Issue: 8 Pages: e1002245-e1002245
10.1371/journal.pgen.1002245
http://house.rbc.kyoto-u.ac.jp/late-effect
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