Analysis of polyoma virus related tumor: Merlkel cell polyomavirus infection and tumorgenesis

• Project/Area Number: 23590389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Chiba University
• Principal Investigator: OTA Satoshi 千葉大学, 医学部附属病院, 准教授 (90324342)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: メルケル細胞ポリオーマウイルス / 皮膚腫瘍 / メルケル細胞癌 / ポリオーマウイルス / リンパ増殖病変 / リンパ増殖疾患 / 基底細胞癌

Research Abstract

Merkel cell polyomavirus (MCPyV) has recently been identified in Merkel cell carcinoma (MCC), an aggressive cancer that occurs in sun-exposed skin. We performed quantitative analyses of the MCPyV copy number in various skin tumor tissues, including MCC and other sun exposure-related skin tumors. In a conventional PCR analysis, MCPyV DNA was detected in all 9 MCCs, 1 Basal cell carcinoma, and 3 Actinic Keraotis. The viral copy number per haploid genome was estimated to be around 1 in most MCC tissues, and there were marked differences between the MCC and AK groups by digital PCR technology. PCR-positive BCC tissue showed a similar viral load as MCC tissue. Immunohistochemistry with a monoclonal antibody against the MCPyV T antigen demonstrated positive nuclear localization in most of the high-viral-load tumor groups, but not in the low-viral-load or PCR-negative tumor groups. These results demonstrated that MCPyV infection is possibly involved in a minority of sun-exposed skin tumors.

Research Products

• [Journal Article] Primary neuroendocrine carcinoma of ocular adnexa (2013)
  • Author(s): Yamanouchi D, Oshitari T, Nakamura Y, Yotsukura J, Asanagi K, Baba T, Nizawa T, Kishimoto T, Yonemori Y, Ota S, Yamamoto S
  • Journal Title: Case Rep Ophthalmol Med Volume: 2013 Pages: 281351-281351
• [Journal Article] ポリオーマウイルスとヒト腫瘍 (2013)
  • Author(s): 太田聡
  • Journal Title: 病理と臨床 Volume: 31 Pages: 159-165
• [Journal Article] Quantitative Analysis of Viral Load per Haploid Genome Revealed the Different Biological Features of Merkel Cell Polyomavirus Infection in Skin Tumor (2012)
  • Author(s): Satoshi Ota, Shumpei Ishikawa2, Yutaka Takazawa, Akiteru Goto, Takeshi Fujii, Ken-ichi Ohashi, Masashi Fukayama
  • Journal Title: PLoS One Volume: 7(6)
• [Journal Article] Quantitative analysis of viral load per haploid genome revealed the different biological features of merkel cell polyomavirus infection in skin tumor (2012)
  • Author(s): Ota, S., Ishikawa, S., Takazawa, Y., Goto, A., Fujii, T., Ohashi, K., Fukayama, M
  • Journal Title: PLOS ONE. Volume: 7 Issue: 6 Pages: e39954-e39954
  • DOI: 10.1371/journal.pone.0039954
  • Peer Reviewed
• [Presentation] 「口演」最近注目される腫瘍ウイルスのトピックスメルケル細胞ポリオーマウイルスと腫瘍 (2011)
  • Author(s): 太田聡, 石川俊平, 深山正久
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜(ワークショップ)
• [Book] ポリオーマウイルスとヒト腫瘍Merkel細胞ポリオーマウイルスを中心に (2013)
  • Author(s): 太田聡
  • Publisher: 病理と臨床