| Project/Area Number |
23590396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMOSHIDA Shingo 神戸大学, 大学院保健学研究科, 教授 (70351020)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUOKA Hiroshi 藤田保健衛生大学, 医学部, 講師 (40367719)
SANGAWA Akiko 大阪赤十字病院, 病理部, 臨床検査技師
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 胃癌 / 大腸癌 / リン酸化カスパーゼ9 / サバイビン / Smac/DIABLO / 細胞死 / 免疫組織化学 / survivin / apoptosis / Autophagy / immunohistochemistry / G2/M期 / 細胞周期 / リン酸化caspase-9 / mitotic catastrophe / 蛍光多重染色 / autophagy / proximity ligation assay / 蛍光多重染色法 |
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| Research Abstract |
Initially, we examined whether phosphorylated caspase 9 is a new marker of mitotic catastrophe. However, this was difficult to prove. Next, we investigated the expression rates of survivin and Smac/DIABLO in gastric and colorectal carcinomas by immunohistochemistry. We observed that gastric and colorectal carcinomas differ in their levels of survivin and Smac/DIABLO expression; both expressions were significantly higher in colorectal carcinoma than in gastric carcinoma. Additionally, we showed that there is a correlation between the expression of Smac/DIABLO and survivin in well-to-moderately differentiated colorectal adenocarcinomas. On the basis of these results, we consider that the expression of Smac/DIABLO in colorectal carcinoma may be upregulated to suppress the anti-apoptotic effect of survivin.
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