| Project/Area Number |
23590408
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
YUKO Kuwae 大阪市立大学, 医学(系)研究科(研究院), 病院講師 (50597557)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAKEHASHI Anna 大阪市立大学, 大学院医学研究科, 講師 (60382222)
WAKASA Kennichi 大阪市立大学, 大学院医学研究科, 登録医 (60158574)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
WANIBUCHI Hideki 大阪市立大学, 大学院医学研究科, 教授 (90220970)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 浸潤性膵管癌 / バイオマーカー / プロテオーム / 早期診断 |
|---|
| Research Abstract |
The present study aimed to identify novel useful clinical biomarker at early stages and to elucidate the molecular background of carcinogenesis in human pancreatic ductal adenocarcinomas (PDACs). Proteomes of dissected PDACs and adjacent non-tumor pancreatic tissues from formalin-fixed and paraffin-embedded sections from 10 patients were analyzed using QSTAR Elite LC-MS/MS, ProteinPilot and Ingenuity Pathway Analysis (IPA). Expression of potential biomarker candidates was validated immunohistochemically in 50 PDAC patients, following by survival analyses and statistical comparison of protein expression with clinicopathological variables.
Paraneoplastic Ma antigen-like 1 (PNMAL1) was selected as a potential biomarker. Immunohistochemical evaluation in 50 PDAC patients revealed that its positive expression was significantly associated with the better overall survival (log-rank test; p=0.009). thus, PNMAL1 is suggested as a novel potential clinically useful prognostic biomarker of PDAC.
|
