| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Aim of the study was to clarify a molecular background of morphogenesis of cellular and structural atypia in thyroid cancer. We established culture system, in which cultured papillary thyroid carcinoma cells artificially formed nuclear grooves (NV) and pseudonuclear inclusion (PNI). Using the culture system, we investigated expression of alpha/beta- and gamma-tubulins, nuclear membrane proteins and cytoskeletons in the cells with NV/PNI. Gamma-tubulins were detected in cytoplasm near NV and in PNI. Also, we studied expression of gamma-tubulin and nuclear membrane proteins in surgically resected human thyroid cancer tissue. Aberrant expression of gamma-tubulin distribution and nuclear membrane proteins was found in the cancer tissue. From the results, we concluded non-centrosomal microtubule organizing center and nuclear membrane proteins played a central role in NV/PNI formation in culture cells and morphogenesis of follicular and papillary structure in human cancer tissue.
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