Hepatocyte chimerism after liver transplantation and allograft aging

• Project/Area Number: 23590424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Kyoto University
• Principal Investigator: MIYAGAWA-HAYASHINO Aya 京都大学, 医学(系)研究科(研究院), 特定准教授 (90432385)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肝移植 / 免疫寛容 / キメリズム / 慢性拒絶 / 病理 / 老化

Research Abstract

Microchimerism after liver transplantation is considered to promote graft tolerance or tissue repair, but its significance is controversial. By using multiplex polymerase chain reaction of short tandem repeat loci after laser capture microdissection of hepatocyte nuclei and fluorescence in situ hybridization of XY chromosome, we compared the proportions of recipient-derived hepatocytes in long-term stable liver allografts and late dysfunctional allografts. In conclusion, hepatocyte chimerism was a constant event. The extent of engraftment of recipient-derived hepatocytes does not seem to correlate with the degree of hepatic injury in long-term liver allografts.

Research Products

• [Journal Article] Redo Living-Donor Lobar Lung Transplantation for Bronchiolitis Obliterans Associated with Antibody-Mediated Rejection (2014)
  • Author(s): Chen F, Miyagawa-Hayashino A, Yurugi K, Chibana N, Yamada T, Sato M, Aoyama A, Takakura S, Bando T, Date H
  • Journal Title: Transpl Int Volume: 27
  • Peer Reviewed
• [Journal Article] Application of complement component 4d immunohistochemistry to ABO-compatible and ABO-incompatible liver transplantation (2014)
  • Author(s): Salah A, Fujimoto M, Yoshizawa A, Yurugi K, Miyagawa-Hayashino A, Sumiyoshi S, Minamiguchi S, Uemoto S, Maekawa T, Haga H
  • Journal Title: Liver Transpl Volume: 20 Issue: 2 Pages: 200-209
  • DOI: 10.1002/lt.23789
  • Peer Reviewed
• [Journal Article] Chronic rejection associated with antiviral therapy for recurrent hepatitis C after living-donor liver transplantation (2014)
  • Author(s): Ueda Y, Kaido T, Ito T, Ogawa K, Yoshizawa A, Fujimoto Y, Mori A, Miyagawa-Hayashino A, Haga H, Marusawa H, Chiba T, Uemoto
  • Journal Title: Transplantation Volume: 97 Issue: 3 Pages: 344-350
  • DOI: 10.1097/01.tp.0000435702.61642.0a
  • Peer Reviewed
• [Journal Article] Redo living-donor lobar lung transplantation for bronchiolitis obliterans associated with antibody-mediated rejection (2014)
  • Author(s): Chen F, Miyagawa-Hayashino A, Yurugi K, Chibana N, Yamada T, Sato M, Aoyama A, Takakura S, Bando T, Date H
  • Journal Title: Transpl Int Volume: 27 Issue: 2 Pages: e8-e12
  • DOI: 10.1111/tri.12224
  • Peer Reviewed
• [Journal Article] Frequent hepatocyte chimerism in long-term human liver allografts independent of graft outcome (2013)
  • Author(s): Aini W, Miyagawa-Hayashino A, Ozeki M, Tsuruyama T, Tamaki K, Uemoto S, Haga H
  • Journal Title: Transpl Immunol Volume: 28 Issue: 2-3 Pages: 100-105
  • DOI: 10.1016/j.trim.2012.12.002
  • NAID: 120005302100
  • Peer Reviewed
• [Journal Article] Significance of semiquantitative assessment of preformed donor-specific antibody using luminex single bead assay in living related liver transplantation (2013)
  • Author(s): Yoshizawa A, Egawa H, Yurugi K, Hishida R, Tsuji H, Ashihara E, Miyagawa-Hayashino A, Teramukai S, Maekawa T, Haga H, Uemoto S
  • Journal Title: Clin Dev Immunol Volume: 2013 Pages: 972705-972705
  • DOI: 10.1155/2013/972705
  • Peer Reviewed
• [Journal Article] Plasma cell hepatitis induced by the termination of antiviral therapy for recurrent hepatitis C after living donor liver transplantation (2013)
  • Author(s): Ueda Y, Yoshizawa A, Ogura Y, Miyagawa-Hayashino A, Haga H, Chiba T, Uemoto S
  • Journal Title: Hepatol Res Volume: (in press) Issue: 10
  • DOI: 10.1111/hepr.12243
  • Peer Reviewed
• [Journal Article] Pretransplant serum hepatitis C virus RNA levels predict response to antiviral treatment after living donor liver transplantation (2013)
  • Author(s): Ueda Y, Kaido T, Ogura Y, Ogawa K, Yoshizawa A, Hata K, Fujimoto Y, Miyagawa-Hayashino A, Haga H, Marusawa H, Teramukai S, Uemoto S, Chiba T
  • Journal Title: PLoS One Volume: 8 Issue: 11
  • DOI: 10.1371/annotation/1601274b-def0-4970-8e61-62e657c2d1ae
  • Peer Reviewed
• [Journal Article] Progressive graft fibrosis and donor-specific HLA antibodies in pediatric late liver allografts (2012)
  • Author(s): Miyagawa-Hayashino A, Yoshizawa A, Uchida Y, Egawa H, Yurugi K, Masuda S, Minamiguchi S, Maekawa T, Uemoto S, Haga H
  • Journal Title: Liver Transpl Volume: 18 Issue: 11 Pages: 1333-1342
  • DOI: 10.1002/lt.23534
  • Peer Reviewed
• [Journal Article] Telomere shortening and karyotypic alterations in hepatocytes in long-term transplanted human liver allografts (2012)
  • Author(s): Aini W, Miyagawa-Hayashino A, Tsuruyama T, Hashimoto S, Sumiyoshi S, Ozeki M, Tamaki K, Uemoto S, Haga H
  • Journal Title: Transpl Int Volume: 25 Issue: 9 Pages: 956-966
  • DOI: 10.1111/j.1432-2277.2012.01523.x
  • Peer Reviewed
• [Journal Article] Liver transplantation for severe hepatitis in patients with common variable immunodeficiency (2012)
  • Author(s): Murakawa Y, Miyagawa-Hayashino A, Ogura Y, Egawa H, Okamoto S, Soejima Y, Kurosawa M, Sumiyoshi S, Uemoto S, Haga H
  • Journal Title: Pediatr Transplant Volume: 16 Issue: 6
  • DOI: 10.1111/j.1399-3046.2011.01545.x
  • Peer Reviewed
• [Journal Article] Non-inflammatory centrilobular sinusoidal fibrosis in pediatric liver transplant recipients under tacrolimus withdrawal (2012)
  • Author(s): Egawa H, Miyagawa-Hayashino A, Haga H, Teramukai S, Yoshizawa A, Ogawa K, Ogura Y, Okamoto S, Kaido T, Uemoto S
  • Journal Title: Hepatol Res Volume: 42 Issue: 9 Pages: 895-903
  • DOI: 10.1111/j.1872-034x.2012.01003.x
  • Peer Reviewed
• [Journal Article] 移植医療移植医療と検査肝移植後の合併症の病理検査と技術 (2012)
  • Author(s): 宮川文[林野], 羽賀博典, 上本伸二
  • Journal Title: Laboratory Practice Volume: 40巻3号 Pages: 202-205
• [Journal Article] Sorafenib attenuates monocrotaline-induced sinusoidal obstruction syndrome in rats through suppression of JNK and MMP-9. (2012)
  • Author(s): Nakamura Koijro
  • Journal Title: J Hepatol. Volume: Nov;57(5) Issue: 5 Pages: 1037-43
  • DOI: 10.1016/j.jhep.2012.07.004
  • Peer Reviewed
• [Journal Article] Effect of maintenance therapy with low-dose peginterferon for recurrent hepatitis C after living donor liver transplantation (2012)
  • Author(s): Ueda Y, Marusawa H, Kaido T, Ogura Y, Oike F, Mori A, Ogawa K, Yoshizawa A, Hatano E, Miyagawa-Hayashino A, Haga H, Egawa H, Takada Y, Uemoto S, Chiba T
  • Journal Title: J Viral Hepat Volume: 19 Issue: 1 Pages: 32-38
  • DOI: 10.1111/j.1365-2893.2010.01398.x
  • Peer Reviewed
• [Journal Article] Frequent overlap of active hepatitis in recurrent primary sclerosing cholangitis after living donor liver transplantation related to its rapidly progressive course (2011)
  • Author(s): Miyagawa-Hayashino A, Egawa H, Yoshizawa A, Ueda Y, Ichida T, Ueno Y, Uemoto S, Harada K, Nakanuma Y
  • Journal Title: Hum Pathol Volume: 42 Pages: 1329-1336
  • Peer Reviewed
• [Journal Article] Progressive familial intrahepatic cholestasis: a single-center experience of living-donor liver transplantation during two decades in Japan (2011)
  • Author(s): Hori T, ８人、Miyagawa-Hayashino A, Yonekawa Y, Yorifuji T, Watanabe KI, Doi H, Nguyen JH, Chen F, Baine AM, Gardner LB, Uemoto S
  • Journal Title: Clin Transplant Volume: 25 Pages: 776-785
  • Peer Reviewed
• [Journal Article] Living-donor Liver Transplantation for Progressive Familial Intrahepatic Cholestasis (2011)
  • Author(s): Hori T, Egawa H, Miyagawa-Hayashino A, Yorifuji T, Yonekawa Y, Nguyen JH, Uemoto S
  • Journal Title: World J Surg Volume: 35 Pages: 393-402
  • Peer Reviewed
• [Presentation] Pleuroparenchymal Fibroelastosis and Non-specific Interstitial Pneumonia as Frequent Pulmonary Disease after Hematopoietic Stem Cell Transplantation (2014)
  • Author(s): Takeuchi Y, Miyagawa-Hayashino A, Chen F, Date H, Haga H
  • Organizer: The 103^<rd> Annual Meeting for United States & Canadian Academy of Pathology
  • Place of Presentation: San Diego, CA, USA
• [Presentation] 肝移植後TMAにおける補体系の関与に関する検討 (2013)
  • Author(s): 田中宏和, 久保田豊成, 秦浩一郎, 影山詔一, 平尾浩史, 岡村裕輔, 宮川文, 和田道彦, 羽賀博典
  • Organizer: 補体シンポジウム講演集(2185-8470)
  • Place of Presentation: 会議録
• [Presentation] 造血幹細胞移植後の肺慢性移植片対宿主病17例に対する病理組織学的検討 (2013)
  • Author(s): 竹内康英, 宮川文, 住吉真治, 陳豊史, 伊達洋至, 羽賀博典
  • Organizer: 日本病理学会会誌(0300-9181)
  • Place of Presentation: 会議録
• [Presentation] 肝移植後の慢性拒絶反応に対する治療と成績 (2013)
  • Author(s): 吉澤 淳、伊藤孝司、小川晃平、藤本康弘、岡本晋也、海道利実、宮川 文、羽賀博典、上本伸二
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
• [Presentation] 小児肝移植患者における de novo 抗ドナー HLA 抗体の検討 (2013)
  • Author(s): 吉澤 淳、小川絵里、岡本晋也、海道利実、宮川 文、羽賀博典、上本伸二
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
• [Presentation] 特発性移植後肝炎における未知の自己抗体の検出 (2013)
  • Author(s): 平田義弘、吉澤 淳、宮川 文、岡本晋也、海道利実、羽賀博典、上本伸二
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
• [Presentation] 造血幹細胞移植後のヒト肺血管内皮における、ドナー ABH 血液型抗原発現についての検討 (2013)
  • Author(s): 土屋恭子、陳豊史、宮川 文、近藤 健、本山秀樹、大角明宏、山田 徹、佐藤雅昭、青山晃博、大政 貢、坂東 徹、伊達洋至
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
• [Presentation] 造血幹細胞移植後の肺慢性移植片対宿主病17例に対する病理組織学的検討 (2013)
  • Author(s): 竹内 康英, 宮川 文, 住吉 真治, 陳 豊史, 伊達 洋至, 羽賀 博典
  • Organizer: 第102回病理学会総会
  • Place of Presentation: 札幌
• [Presentation] The Impact of Donor Specific Antibodies on the Long-Term Liver Allograft after Pediatric Living Donor Liver Transplantation (2012)
  • Author(s): Yoshizawa A, Yurugi K, Hishida R, Miyagawa-Hayashino A, Haga H, Maekawa T, Uemoto S
  • Organizer: 24th International Congress of the Transplantation Society
  • Place of Presentation: Berlin, Germany
• [Presentation] 肝移植後C型肝炎治療の現状と問題点移植 (2012)
  • Author(s): 金秀基, 上田佳秀, 海道利実, 小倉靖弘, 小川晃平, 吉澤淳, 秦浩一郎, 藤本康弘, 伊藤孝司, 丸澤宏之, 宮川文, 羽賀博典, 千葉勉, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] 免疫抑制剤完全離脱症例の経過の検討移植 (2012)
  • Author(s): 平田義弘, 吉澤淳, 宮川文, 吉利幸江, 藤本康弘, 小川晃平, 小倉康弘, 岡本晋也, 羽賀博典, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] 劇症肝炎に対し生体肝移植後、GVHDを発症した症例移植 (2012)
  • Author(s): 金城昌克, 吉澤淳, 宮川文, 吾拉木江艾尼, 岡本晋弥, 羽賀博典, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] 長期経過小児肝移植患者における晩期拒絶反応と抗ドナーHLA抗体の検討移植 (2012)
  • Author(s): 吉澤淳, 宮川文, 岡本晋也, 海道利実, 羽賀博典, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] 臓器移植における抗HLA抗体の意義肝移植におけるC4d免疫染色の臨床病理学的意義移植 (2012)
  • Author(s): 羽賀博典, サーレハ・アディーブ, 吉澤淳, 宮川文, 藤本正数, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] グラフト肝生検におけるC4d免疫染色と術後抗ドナー抗体との比較検討 (2012)
  • Author(s): 羽賀博典, 福田裕紀, 藤本正数, サーレハ・アディーブ, 住吉真治, 南口早智子, 宮川文
  • Organizer: 日本病理学会会誌
  • Place of Presentation: 会議録
• [Presentation] 移植小腸の粘膜免疫と組織診断 (2012)
  • Author(s): 鶴山竜昭, 宮川文, 南口早智子, 三上芳喜, 上本伸二, 羽賀博典
  • Place of Presentation: 会議録
• [Presentation] The Impact of Donor Specific Antibodies on the Long-Term Liver Allograft after Pediatric Living Donor Liver Transplantation (2012)
  • Author(s): Yoshizawa A, Yurugi K, Hishida R, Miyagawa-Hayashino A, Haga H, Maekawa T, and Uemoto S
  • Organizer: 24th International Congress of the Transplantation Society
  • Place of Presentation: Berlin
• [Presentation] 移植小腸の粘膜免疫と組織診断 (2012)
  • Author(s): 鶴山竜昭、宮川 文、南口早智子、三上芳喜、羽賀博典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] グラフト肝生検におけるC4d免疫染色と術後抗ドナー抗体との比較検討 (2012)
  • Author(s): 羽賀博典、福田祐紀、藤本正数、サーレハ アディーブ、住吉真治、南口早智子、宮川 文
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 生体肝移植における術前リンパ球クロスマッチテストと抗ドナーHLA抗体検出の意義移植 (2011)
  • Author(s): 吉澤淳, 江川裕人, 菱田理恵, 万木紀美子, 芦原英司, 宮川文, 飯田拓, 八木真太郎, 秦浩一郎, 小倉靖弘, 森章, 海道利実, 前川平, 羽賀博典, 上本信二
  • Place of Presentation: 会議録
• [Presentation] HCV陽性レシピエントに対する肝移植後の長期予後移植 (2011)
  • Author(s): 上田佳秀(京都大学消化器内科), 海道利実, 小倉靖弘, 森章, 小川晃平, 吉澤淳, 秦浩一郎, 八木真太郎, 宮川文, 羽賀博典, 丸澤宏之, 千葉勉, 上本伸二
  • Place of Presentation: 会議録
• [Presentation] 抗体関連型拒絶反応の診断に苦慮した細胞障害性クロスマッチ陰性の生体肝移植後の1例 (2011)
  • Author(s): 吉澤淳(京都大学医学部附属病院肝胆膵・移植外科), 江川裕人, 菱田理恵, 万木紀美子, 宮川文, 前川平, 羽賀博典, 上本信二
  • Place of Presentation: 会議録
• [Presentation] Major Histocompatibility Complex (2011)
  • Author(s): MHC
  • Place of Presentation: 会議録/症例報告
• [Presentation] 生体肝移植における術前抗ドナーHLA抗体陽性の意義 (2011)
  • Author(s): 吉澤淳(京都大学肝胆膵・移植外科), 江川裕人, 菱田理恵, 万木紀美子, 芦原英司, 宮川文, 飯田拓, 八木真太郎, 秦浩一郎, 小倉靖弘, 森章, 海道利美, 羽賀博典, 前川平, 上本伸二
  • Organizer: 日本外科学会雑誌
  • Place of Presentation: 会議録