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Analysis for acetylation status of microtubules in epithelial mesenchymal transition induced by HGF/MET stimulation

Research Project

Project/Area Number 23590431
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionIwate Medical University

Principal Investigator

MASUDA Tomoyuki  岩手医科大学, 医学部, 教授 (10199698)

Co-Investigator(Kenkyū-buntansha) 柴崎 晶彦  岩手医科大学, 医学部, 助教 (20445109)
前沢 千早  岩手医科大学, 医学部, 教授 (10326647)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords肝癌 / HDAC6 / アセチル化 / 微小管 / EMT / 分子病理 / 肝細胞癌 / HGF / MET / TGFβ
Research Abstract

The aim of this study was to investigate the significance of HDAC6 in the invasion and metastasis activities of hepatocellular carcinoma (HCC). And we also examined the effect on epithelial mesenchymal trsnsition induced by HGF/MET system. HDAC6 expression was greater in all of the HCC cell lines compared to the primary cultures of hepatocytes. Knockdown of HDAC6 markedly downregulated the migration and invasion activities of all HCC cell lines (P<0.05). Overexpression of HDAC6 protein to a level higher than that in the corresponding normal hepatocytes was observed in 14 (20%) of the 70 primary HCCs, and was significantly correlated with high clinical stage, number of tumors, vascular invasion and intrahepatic metastasis (P<0.05). Downregulation of HDAC6 inhibited ERK1/2 tranlocation to the nuclesu. These results suggest that overexpression of the HDAC6 protein is involved in EMT of HCC cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (12 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results)

  • [Journal Article] Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.2014

    • Author(s)
      Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, Maeda F, Takahashi K, Akasaka T, Masuda T. Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.
    • Journal Title

      Am J Dermatopathol

      Volume: 36(3) Issue: 3 Pages: 211-216

    • DOI

      10.1097/dad.0b013e3182964e02

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A disintegrin and metalloproteinase 17 (ADAM17) mediates epidermal growth factor receptor transactivation by angiotensin II on hepatic stellate cells.2014

    • Author(s)
      Oikawa H, Maesawa C, Tatemichi Y, Nishinari Y, Nishiya M, Mizugai H, Ikeda A, Oikawa K, Takikawa Y, Masuda T.
    • Journal Title

      Life Sci.

      Volume: 97(2) Issue: 2 Pages: 137-144

    • DOI

      10.1016/j.lfs.2013.12.028

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A somatic mutation of the KEAP1 gene in malignant melanoma is involved in aberrant NRF2 activation and an increase in intrinsic drug resistance.2014

    • Author(s)
      Miura S, Shibazaki M, Kasai S, Yasuhira S, Watanabe A, Inoue T, Kageshita Y, Tsunoda K, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      J Invest Dermatol.

      Volume: 134(2) Issue: 2 Pages: 553-556

    • DOI

      10.1038/jid.2013.343

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) with hemophagocytosis (HPS): successful treatment using high-dose chemotherapy (BFM-NHL & ALL-90) and autologous peripheral blood stem cell transplantation2013

    • Author(s)
      Sakurai E, Satoh T, Akiko YA, Maesawa C, Tsunoda K, Endo M, Akasaka T, Masuda T
    • Journal Title

      J Clin Exp Hematop

      Volume: 53(2) Pages: 135-40

    • Related Report
      2013 Final Research Report
  • [Journal Article] BCL2 and BCLxL are key determinants of resistance to antitubulin chemotherapeutics in melanoma cells.2013

    • Author(s)
      Watanabe A, Yasuhira S, Inoue T, Kasai S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      Exp Dermatol

      Volume: 22(8) Issue: 8 Pages: 518-523

    • DOI

      10.1111/exd.12185

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loss of HOXD10 expression induced by upregulation of miR -10b accelerates themigration and invasion activities of ovarian cancer cells2013

    • Author(s)
      Nakayama I, Shibazaki M, Yashima-Abo A, Miura F, Sugiyama T, Masuda T, MaesawaC
    • Journal Title

      Int J Oncol

      Volume: 43(1) Issue: 1 Pages: 63-71

    • DOI

      10.3892/ijo.2013.1935

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) with hemophagocytosis (HPS): successful treatment using high-dose chemotherapy (BFM-NHL & ALL-90) and autologous peripheral blood stem cell transplantation.2013

    • Author(s)
      Sakurai E, Satoh T, Akiko YA, Maesawa C, Tsunoda K, Endo M, Akasaka T, Masuda T.
    • Journal Title

      J Clin Exp Hematop.

      Volume: 53(2) Pages: 135-140

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpre ssion of histone deacetylase 6 contributes to accelerated migration and invasion activity of hepatocellular carcinoma cells.2012

    • Author(s)
      Kanno K, Kanno S, Nitta H, Uesugi N, Sugai T, Masuda T, Wakabayashi G , Maesawa C
    • Journal Title

      Oncol Rep.

      Volume: 28(3) Issue: 3 Pages: 867-73

    • DOI

      10.3892/or.2012.1898

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity2012

    • Author(s)
      Ishikawa Y, Tsunoda K, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      Int J Oncol

      Volume: 41(1) Pages: 53-60

    • DOI

      10.3892/ijo.2012.1424

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sensor and effector kinases in DNAdamage checkpoint regulate capacity for homologous recombination repair offission yeast in G2 phase.2012

    • Author(s)
      Yasuhira S, Saito T, Maesawa C, Masuda T.
    • Journal Title

      DNA Repair (Amst).

      Volume: 11(8) Issue: 8 Pages: 666-75

    • DOI

      10.1016/j.dnarep.2012.05.006

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sternoclavicular joint septic arthritis following paraspinal muscle abscess and septic lumbar spondylodiscitis with epidural abscess in a patient with diabetes: a case report.2012

    • Author(s)
      Shioya N, Ishibe Y, Kan S, Masuda T, Matsumoto N, Takahashi G, Makabe H, Yamada Y, Endo S.
    • Journal Title

      BMC Emerg Med

      Volume: 12 Pages: 7-7

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpression of histone deacetylase 6 contributes to accelerated migration/invasion activity of hepatocellular carcinoma cells.2012

    • Author(s)
      Kanno K
    • Journal Title

      Oncol Rep

      Volume: in press

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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