| Project/Area Number |
23590440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
AIDA Junko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京と健康長寿医療センター研究所, 研究員 (80425678)
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| Co-Investigator(Renkei-kenkyūsha) |
IZUMIYAMA Naotaka 地方独立行政法人東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 老年病理学研究チーム・助手 (10158751)
NAKAMURA Ken-ichi 地方独立行政法人東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 老年病理学研究チーム・研究員 (60159069)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分子病理 / テロメア / Q-FISH / 膵β細胞 / 膵α細胞 / 2型糖尿病 / 加齢 / 老化 / β細胞 / α細胞 / HbA1c / 糖尿病 / 膵 / Q-FISH法 / 蛍光抗体法 / 国際研究者交流 / カナダ |
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| Research Abstract |
In type 2 diabetes, reduction of beta cells causes dysfunction of the glucose tolerance. Telomere is related to malignant neoplasms or geriatric diseases. Therefore, we predicted that telomeres of beta cells should be shorten selectively in diabetic pancreas.
Tissue Q-FISH method was applied to paraffin-embedded pancreatic sections from autopsy cases (type 2 diabetes 47 cases and control 104 cases). Telomere lengths of each cell group of the pancreas were measured and compared. Alpha and beta cells in pancreatic islets were distinguished by superimposed immunofluorescence study for glucagon and insulin.
Telomere lengths were longest in the nerve cells, and longer in ducts than exocrine or endocrine glands. In the diabetic cases, almost cell group showed shorter telomeres than the controls, but most prominent especially in beta cells. Therefore, it is suggested that telomere shortening in beta cells has relevance to occurrence of type 2 diabetes.
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