Clinical application and biochemical analysis of the citrullinated proteins which catalyzed by peptidylarginine deiminase
Project/Area Number |
23590441
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
|
Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
Principal Investigator |
MARUYAMA Naoki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (00115940)
|
Co-Investigator(Kenkyū-buntansha) |
KAGAMI Yayoi 地方独立行政法人東京都健康長寿医療センター, 研究所, 研究員 (80557661)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 酵素 / 蛋白質 / 癌 / シトルリン / PAD / シトルリン化 / 測定系 |
Research Abstract |
We developed two novel methods for sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of human peptidylarginine deiminase 4 (hPAD4) and the detection of hPAD4 autoantibodies in the peripheral blood. The combination of these sandwich ELISA methods might be a potentially beneficial clinical tool for diagnosing RA.
|
Report
(4 results)
Research Products
(49 results)
-
[Journal Article] Peptidylarginine deiminase and protein citrullination in prion diseases2013
Author(s)
Jang, B., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
-
Journal Title
Strong evidence of neurodegeneration
Volume: Prion 7
Pages: 42-46
Related Report
-
[Journal Article] Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis2013
Author(s)
Ishigami, A., Uchida, Y., Miyazaki, T., Handa, S., Choi, E.K,, Kim, Y.S,, Kasahara, Y., Maruyama, N.
-
Journal Title
Mod Rheumatol
Volume: 23
Pages: 794-803
NAID
Related Report
-
[Journal Article] Peptidylarginine deiminase and protein citrullination in prion diseases: Strong evidence of neurodegeneration.2013
Author(s)
Jang, B., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
-
Journal Title
Prion
Volume: 7
Issue: 1
Pages: 42-46
DOI
Related Report
Peer Reviewed
-
[Journal Article] Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis.2013
Author(s)
Ishigami, A., Uchida, Y., Miyazaki, T., Handa, S., Choi, E.K,, Kim, Y.S,, Kasahara, Y., Maruyama, N.
-
Journal Title
Mod Rheumatol.
Volume: 23
Issue: 4
Pages: 794-803
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination : links between altered multifunction of enolase and neurodegenerative diseases2012
Author(s)
Jang, B., Jeon, Y.C., Choi, J.K., Park, ., Kim, J.I., Ishigami, A., Maruyama, ., Carp, R.I., Kim, Y.S., Choi, E.K.
-
Journal Title
Biochem. J
Volume: 445
Pages: 183-192
Related Report
-
[Journal Article] Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis.2012
Author(s)
Ishigami, A., Uchida, Y., Miyazaki, T., Handa, S., Choi, E.K,, Kim, Y.S,, Kasahara, Y., Maruyama, N.
-
Journal Title
Mod Rheumatol
Volume: in press
NAID
Related Report
Peer Reviewed
-
[Journal Article] Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination: links between altered multifunction of enolase and neurodegenerative diseases.2012
Author(s)
Jang, B., Jeon, Y.C., Choi, J.K., Park, M., Kim, J.I., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
-
Journal Title
Biochem J
Volume: 445
Pages: 183-192
Related Report
Peer Reviewed
-
[Journal Article] 3,4-diaminopyridine improves neuromuscular transmission in a MuSK antibodies-induced mouse model of myasthenia gravis.2012
Author(s)
Mori, S., Kishi, M., Kubo, S., Akiyoshi, T., Yamada, S., Miyazaki, T., Konishi, T., Maruyama, N., Shigemoto, K.
-
Journal Title
J Neuroimmunol
Volume: 245
Pages: 75-78
Related Report
Peer Reviewed
-
[Journal Article] Antibodies against Muscle-Specific Kinase Impair Both Presynaptic and Postsynaptic Functions in a Murine Model of Myasthenia Gravis.2012
Author(s)
Mori, S., Kubo, S., Akiyoshi, T., Yamada, S., Miyazaki, T., Hotta, H., Desaki, J., Kishi, M., Konishi, T., Nishino, Y., Miyazawa, A., Maruyama, N., Shigemoto, K.
-
Journal Title
Amr J Pathol
Volume: 180
Pages: 798-810
Related Report
Peer Reviewed
-
[Journal Article] Subcellular localization of peptidylarginine deiminase 2 and citrullinated proteins in brains of scrapie-infected mice : nuclear localization of PAD2 and membrane fraction-enriched citrullinated proteins2011
Author(s)
Jang, B., Shin, HY., Choi, JK., Nguyen, du PT., Jeong, BH., Ishigami, A., Maruyama, N., Carp, RI., Kim, YS., and Choi, EK.
-
Journal Title
J. Neuropathol. Exp. Neurol
Volume: 70
Pages: 116-124
Related Report
-
[Journal Article] Shimada, S., Tanaka, Y., Waki, H., Kon, K., Iwamoto, M., Maruyama, N.: Analysis of brain cell activation by nanosized particles of Ginkgo biloba extract.2011
Author(s)
Shimada, S., Tanaka, Y., Waki, H., Kon, K., Iwamoto, M., Maruyama, N.
-
Journal Title
International Journal of Plant Physiology and Biochemistry
Volume: 3
Pages: 28-33
Related Report
Peer Reviewed
-
[Journal Article] 17beta-Estradiol attenuates saturated fatty acid diet-induced liver injury in ovariectomized mice by up-regulating hepatic senescence marker protein-30.2011
Author(s)
Fukui, M., Senmaru, T., Hasegawa, G., Yamazaki, M., Asano, M., Kagami, Y., Ishigami, A., Maruyama, N., Iwasa, K., Kitawaki, J., Itoh, Y., Okanoue, T., Ohta, M., Obayashi, H., Nakamura, N.
-
Journal Title
Biochem Biophys Res Commun
Volume: 415
Pages: 252-257
Related Report
Peer Reviewed
-
[Journal Article] Absorption and excretion of ascorbic acid alone and in acerola (Malpighia emarginata) juice: comparison in healthy Japanese subjects.2011
Author(s)
Uchida, E., Kondo, Y., Amano, A., Aizawa, S., Hanamura, T., Aoki, H., Nagamine, K., Koizumi, T., Maruyama, N., Ishigami, A.
-
Journal Title
Biol Pharm Bull
Volume: 34
Pages: 1744-1747
NAID
Related Report
Peer Reviewed
-
[Journal Article] Establishment and characterization of hepatocytes from an Immortomouse/SMP30/GNL knockout mouse hybrid lacking vitamin C to study vitamin C transport.2011
Author(s)
Amano, A., Handa, S., Aigaki, T., Shigemoto, K., Maruyama, N., Ishigami, A.
-
Journal Title
J Biochem
Volume: 150
Pages: 671-678
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Mitochondrial reactive oxygen species generation by the SDHC V69E mutation causes low birth weight and neonatal growth retardation.2011
Author(s)
Ishii, T., Miyazawa, M., Onodera, A., Yasuda, K., Kawabe, N., Kirinashizawa, M., Yoshimura, S., Maruyama, N., Hartman, P.S., Ishii, N.
-
Journal Title
Mitochondrion
Volume: 11
Pages: 155-165
Related Report
Peer Reviewed
-
[Journal Article] Subcellular localization of peptidylarginine deiminase 2 and citrullinated proteins in brains of scrapie-infected mice: nuclear localization of PAD2 and membrane fraction-enriched citrullinated proteins.2011
Author(s)
Jang, B., Shin, H.Y., Choi, J.K., Nguyen du, P.T., Jeong, B.H., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
-
Journal Title
J Neuropathol Exp Neurol
Volume: 70
Pages: 116-124
Related Report
Peer Reviewed
-
-
-
-
-
[Presentation] Age-related changes of peptidylarginine deiminase 2 in the mouse brain2013
Author(s)
Kagami, Y., Shimada, N., Handa, S., Choi, E.K., Kim, Y.S., Ito, H., Maruyama, N., Ishigami, A.
Organizer
The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
Place of Presentation
Seoul, Korea
Year and Date
2013-06-26
Related Report
-
[Presentation] Peptidylarginine deiminase 2-mediat ed protein citrullinati on in prion disease2013
Author(s)
Kim, H.J., Jang, B., Jin, J.K., Jeon, Y.C., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
Organizer
The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
Place of Presentation
Seoul, Korea
Year and Date
2013-06-26
Related Report
-
-
-
[Presentation] Age-related changes of peptidylarginine deiminase 2 in the mouse brain.2013
Author(s)
Kagami, Y., Shimada, N., Handa, S., Choi, E.K., Kim, Y.S., Ito, H., Maruyama, N., Ishigami, A.
Organizer
The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
Place of Presentation
Seoul, Korea
Related Report
-
[Presentation] Peptidylarginine deiminase 2-mediat ed protein citrullinati on in prion disease.2013
Author(s)
Kim, H.J., Jang, B., Jin, J.K., Jeon, Y.C., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
Organizer
The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
Place of Presentation
Seoul, Korea
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Evaluation of antioxidant activity of Olive Oil Potato Chips after oral administration in vitamin C-deficient SMP30/GNL knockout mice.2011
Author(s)
Sakuma,R., Kondo, Y., Ichisawa, M., Ishihara, K., Kubo, M., Ohashi, M., Handa, S., Mugita, H., Maruyama, N., Koga, H., Ishigami, A.
Organizer
International Society for Nutraceuticals and Functional Foods (ISNFF)
Place of Presentation
Sapporo
Related Report
-
[Presentation] Effect of vitamin C deficiency on noradrenaline synthesis.2011
Author(s)
Amano, A., Tsunoda, M., Aigaki, T., Maruyama, N., Ishigami, A.
Organizer
The 9th Asia/Oceania Regional Congress of Gerontology
Place of Presentation
Melbourne, Australia,
Related Report
-
-
-
-
-
-
-
-