The effect of EB virus infection in nasopharyngeal carsinoma cancer stem cell
| Project/Area Number |
23590443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
IIZASA Hisashi 北海道大学, 遺伝子病制御研究所, 助教 (80306662)
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| Co-Investigator(Renkei-kenkyūsha) |
IWAKIRI Dai 北海道大学, 遺伝子病制御研究所, 助教 (10307853)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | がん幹細胞 / EBウイルス / SOX2 / プロモーター / 幹細胞 / EBウィルス |
|---|
| Research Abstract |
We had tried to establish EB virus (EBV) negative cell lines from EBV harboring human nasopharyngeal carcinoma cell line C666-1. C666-1 had a large number of EBV copies per cell and we could not establish EBV negative cell line from C666-1 using drug treatment and dilution cloning. These results suggest that EBV infection is essential to keep C666-1 cell proliferation. Next, to isolate cancer stem cell population from nasopharyngeal carcinoma, we analyzed the expression mechanism of stem cell specific transcription factor SOX2 in cancer cell lines. SOX2 promoter activity was related to SOX2 mRNA expression level and promoter positive cell population showed high sphere activity. Moreover, we identified SOX2 promoter binding protein and this protein regulated cancer stem cell formation by the regulation of SOX2 promoter activity.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Enhancement of in vitro cell motility and invasiveness of human malignant pleural mesothelioma cells through the HIF-1α-MUC1 pathway2014
Author(s)
Goudarzi H, Iizasa H, Furuhashi M, Nakazawa S, Nakane R, Liang S, Hida Y, Yanagihara K, Kubo T, Nakagawa K, Kobayashi M, Irimura T, Hamada J.
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Journal Title
Cancer Letters
Volume: 339
Pages: 169-174
DOI
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Peer Reviewed
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[Journal Article] A novel animal model of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis in humanized mice.2011
Author(s)
Sato K, Misawa N, Nie C, Satou Y, Iwakiri D, Matsuoka M, Takahashi R, Kuzushima K, Ito M, Takada K, Koyanagi Y
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Journal Title
BLOOD
Volume: 117
Pages: 5663-5673
DOI
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Peer Reviewed
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[Presentation] MUC-1 plays an important role in enhancement motility and invasiveness of human malignant mesothelioma cells under hypoxia.2012
Author(s)
Goudarzi H, Iizasa H, Furuhashi M, Nakane R, Nakazawa S, Liang S, Yanagihara K, Kubo T, Nakagawa K, Kobayashi M, Hida Y, Irimura T, Hamada J.
Organizer
International Symposium on Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
Place of Presentation
Ito hall (Internatinal Research Center)（Tokyo）
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[Presentation] ウィルス由来microRNAによるウィルス潜伏感染調節機構の解析2011
Author(s)
飯笹久, Bjorn-Erik Wulff, Manolis Maragkakis, Molly Megraw, Artemis Hatzigeorgious, 岩切大, 高田賢蔵, Andreas Wiedmer, Louis Showe, Paul Lieberman, 西倉和子
Organizer
第５回北大若手研究者交流会
Place of Presentation
北海道大学医学部フラテ会館（札幌）
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