| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The roles of platelet-derived growth factor receptor beta (PDGFR-beta) in synaptogenesis were examined using primary cultured mouse hippocampal neurons. PDGFR-beta conditional knockout hippocampal neurons showed lower motility of dendritic filopodia and lower numbers of spine as compared with PDGFR-beta preserved control neurons. mRNA expressions of RhoA, VASP, PAL1, ARPC3 in PDGFR-beta knockout neurons were significantly lower than those of control cells. It is suggested that PDGFR-beta has important role in synaptogenesis through these cytoskeleton related genes. We also demonstrated the neuroprotective effects of PDGF-B against oxidative stress using cultured mouse cortical neurons. PDGF-B significantly suppressed intracellular Calcium-ion overload and inactivation of calpain induced by oxidative stress. Present study supports the notion that PDGF-B/PDGFR-beta may be a potential therapeutic target of neurological diseases and synaptogenesis.
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