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The analysis of the new inhibitory factor for cytomegalovirus in ES/iPS cells

Research Project

Project/Area Number 23590445
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

KAWASAKI Hideya  浜松医科大学, 医学部, 助教 (90397381)

Co-Investigator(Kenkyū-buntansha) KOSUGI Isao  浜松医科大学, 医学部, 准教授 (10252173)
ARAI Yoshihumi  浜松医科大学, 医学部, 助教 (30381784)
IWASHITA Toshihide  浜松医科大学, 医学部, 教授 (00283432)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsES / iPS / サイトメガロウイルス / β1 インテグリン / 大脳 / レセプター / ES細胞
Research Abstract

Pluripotent stem cell (iPS/ES cells) showed resistance to CMV. We established iPS cells from human fibroblast using Yamanka factors. iPS showed remarkable resistance to CMV infection. This is the compatible with previous report (Plos one: 10.1371/journal.pone.0017492). We tried to investigate the new factor for this inhibitory phenomenon.The new factor has not been found yet. Instead, we tried to find known receptor (CD29) distribution for CMV in brain. Endothelial cells, pericytes, meninges, choloid plexus and NSPC all express higher level of CD29 than other area of the brain. Co-expression of higher level of CD29 expressing cells and MCMV infected cells were highly correlated. In the brain. We concluded that cytomegalovirus infection primarily initiates infection from high-level CD29 expressing cells in acute infection phase.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2013 2012 2011

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (11 results)

  • [Journal Article] Establishment of a novel mouse model of ulcerative colitis with concomitant cytomegalovirus infection - in vivo identification of cytomegalovirus persistent infected cells2013

    • Author(s)
      Matsumura K, Nakase H, Kosugi I, Honzawa Y, Yoshino T, Matsuura M, Kawasaki H, Arai Y, Iwashita T, Nagasawa T, Chiba T
    • Journal Title

      Inflammatory Bowel Disease

      Volume: 19(9) Pages: 1951-1956

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences.2013

    • Author(s)
      Akamatsu T, Arai Y, Kosugi I, Kawasaki H, Meguro S, Sakao M, Shibata K, Suda T, Chida K, Iwashita T
    • Journal Title

      Fibrogenesis Tissue Repair

      Volume: 6 Issue: 1 Pages: 15-15

    • DOI

      10.1186/1755-1536-6-15

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An autopsy case of the marburg variant of multiple sclerosis (acute multiple sclerosis)2013

    • Author(s)
      Suzuki M, Kawasaki H, Masaki K, Suzuki S, Terada T, Tsuchida T, Tokuyama T, Kono S, Komori T, Baba S, Kira J, Miyajima H
    • Journal Title

      Intern Med.

      Volume: 52(16) Pages: 1825-1832

    • NAID

      130003365755

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression ofβ-adrenoceptor subtypes in urothelium, interstitial cells and detrusor of the human urinary bladder2013

    • Author(s)
      Otsuka A, Kawasaki H, Matsumoto R, Shinbo H, Kurita Y, Iwashita T, Ozono S
    • Journal Title

      LUTS

      Volume: 5 Pages: 173-180

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a novel mouse model of ulcerative colitis with concomitant cytomegalovirus infection - in vivo identification of cytomegalovirus persistent infected cells,2013

    • Author(s)
      Matsumura K, Nakase H, Kosugi I, Honzawa Y, Yoshino T, Matsuura M, Kawasaki H, Arai Y, Iwashita T, Nagasawa T, Chiba T.
    • Journal Title

      Inflammatory Bowel Disease

      Volume: 19 Pages: 1951-1956

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotent stem cells are protected from cytomegalovirus infection at multiple points : implications of a new pathogenesis for congenital anomaly caused by cytomegalovirus2012

    • Author(s)
      Kawasaki H
    • Journal Title

      Congenit Anom

      Volume: 52(Review) Pages: 147-154

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of intrapulmonary viral tropism and cytokine expression on the histological patterns of cytomegalovirus pneumonia2012

    • Author(s)
      Arai Y, Tsuchida T, Kosugi I, Kawasaki H, Meguro S, Kinoshita M, Baba S, Maeda M, Shinmura Y, Tsutsui Y, Iwashita T
    • Journal Title

      Pathol Int

      Volume: 62 Pages: 628-639

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotent stem cells are protected from cytomegalovirus infection at multiple points: implications of a new pathogenesis for congenital anomaly caused by cytomegalovirus2012

    • Author(s)
      Kawasaki H
    • Journal Title

      Congenit. Anom(Kyoto)

      Volume: 52 Issue: 3 Pages: 147-154

    • DOI

      10.1111/j.1741-4520.2012.00375.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of intrapulmonary viral tropism and cytokine expression on histoligical patterns of cytomegalovirus infection2012

    • Author(s)
      Arai Y, Tsuchida T, Kosugi K, Kawasaki H, Meguro S, Kinoshita M, Baba B, Maeda , Shinmura Y, Tsutsui Y. Iwashita T
    • Journal Title

      Pathology International

      Volume: 62 Issue: 9 Pages: 628-639

    • DOI

      10.1111/j.1440-1827.2012.02849.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of β-adrenoceptor subtypes in urothelium, interstitial cells and detrusor of the human urinary bladder2012

    • Author(s)
      Otsuka A,Kawasaki H, Matsumoto R, Shinbo H, Kurita Y, Iwashita T, Ozono S
    • Journal Title

      LUTS:Lower Urinary Tract Symptoms

      Volume: 未定

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process2011

    • Author(s)
      Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process.2011

    • Author(s)
      Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y.
    • Journal Title

      PloS One

      Volume: 2;6(3)

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細胞とintegrinβ1との関係2013

    • Author(s)
      河崎秀陽、鈴木万幾子、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
    • Organizer
      第61回ウイルス学会
    • Place of Presentation
      神戸
    • Year and Date
      2013-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細胞とintegrinβ1との関係2013

    • Author(s)
      河崎秀陽、鈴木万幾子、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
    • Organizer
      第102回日本病理学会
    • Place of Presentation
      札幌
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細 胞とintegrinβ1との関係2013

    • Author(s)
      河崎 秀陽、鈴木 万幾子、小杉 伊三夫、新井 義文、筒井 祥博、岩下 寿秀
    • Organizer
      日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 日本ウイルス学会2013

    • Author(s)
      河崎 秀陽、鈴木 万幾子、小杉 伊三夫、新井 義文、筒井 祥博、岩下 寿秀
    • Organizer
      急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細胞とi ntegrin β1との関係
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrinβ1発現と感染分布2012

    • Author(s)
      河崎秀陽、小杉伊三夫、鈴木万幾子、新井義文、筒井祥博、岩下寿秀
    • Organizer
      第52回日本先天異常学会
    • Place of Presentation
      東京
    • Year and Date
      2012-07-06
    • Related Report
      2013 Final Research Report
  • [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrinβ1発現と感染分布2012

    • Author(s)
      河崎秀陽、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
    • Organizer
      第101会日本病理学会
    • Place of Presentation
      東京
    • Year and Date
      2012-04-26
    • Related Report
      2013 Final Research Report
  • [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrin β1発現と感染分布2012

    • Author(s)
      河崎 秀陽, 小杉 伊三夫, 新井 義文, 筒井 祥博, 岩下 寿秀
    • Organizer
      第101会日本病理学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrin β1発現と感染分布との関係2012

    • Author(s)
      河崎 秀陽, 小杉 伊三夫, 鈴木 万幾子, 新井 義文, 筒井 祥博, 岩下 寿秀
    • Organizer
      第52回日本先天異常学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Mouse Embryonic Stem Cells inhibit Cyotomegalovirus Infection Through a Multi-Step Process2011

    • Author(s)
      Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y
    • Organizer
      International Congress of Virology
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 幹細胞に着目したサイトメガロウイルス感染感受性の解析2011

    • Author(s)
      河崎秀陽
    • Organizer
      第100回病理学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-04-28
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] MOUSE EMBRYONIC STEM CELLS INHIBIT MURINE CYTOMEGALOVIRUS INFECTION THROUGH A MULTI-STEP PROCESS2011

    • Author(s)
      Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y.
    • Organizer
      International Congress of Virology
    • Place of Presentation
      Sapporo
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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