Regulation of microRNA by stimulation of growth factors in various cancer cells

• Project/Area Number: 23590455
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Yamaguchi University (2012-2013); University of Fukui (2011)
• Principal Investigator: ITOH HIROSHI 山口大学, 医学(系)研究科(研究院), 教授 (80253847)
• Co-Investigator(Renkei-kenkyūsha): NAGANUMA Seiji 高知大学, 医学部, 助教 (50452123) ; KATAOKA Hiroaki 宮崎大学, 医学部, 教授 (10214321) ; KITAMURA Naomi 東京工業大学, 生命理工学研究科, 教授 (80107424)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マイクロRNA / 細胞増殖因子 / 転写翻訳調節 / HGF / 癌細胞 / 頭頚部癌 / 前立腺癌 / アンドロゲン / 頭頸部癌

Research Abstract

MicroRNAs miR-200c and miR-27b, both of which were down-regulated by HGF stimulation in cultured human head and neck squamous carcinoma cells (HNSCC), might play an important role in EMT mediated by ZEB1 and ECM degradation and HGF auto-activation mediated by ST-14/matriptase, respectively. Altered expression of these microRNAs directly regulated by HGF might contribute enhanced progressive and invasive characteristics of HNSCC by regulating the translation of HGF-induced functional molecules. We also confirmed that the expression of miR-182 depends on the cancer grade even in same Gleason pattern 4 of prostate cancer. Expression of miR-182 associated with Gleason grading system in biopsy sample may contribute to more accurate preoperative cancer risk evaluation.

Research Products

• [Journal Article] Expression of MicroRNAs associated with Gleason grading system in prostate cancer: miR-182-5p is a useful marker for high grade prostate cancer. (2013)
  • Author(s): Tsuchiyama, K., Ito, H., Taga, M., Naganuma, S., Oshinoya, Y., Nagano, K.-i., Yokoyama, O. and Itoh, H.
  • Journal Title: Prostate Volume: 73 Pages: 827-34
  • DOI: 10.1002/pros.22626
  • Peer Reviewed
• [Journal Article] Notch receptor inhibition reveals the importance of cyclin D1 and Wnt signaling in invasive esophageal squamous cell carcinoma (2012)
  • Author(s): Naganuma S, Whelan KA, Natsuizaka M, Kagawa S, Kinugasa H, Chang S, Subramanian H, Rhoades B, Ohashi S, Itoh H, Herlyan M, Diehl JA, Gimotty PA, Klein-Szanto AJ, Nakagawa H
  • Journal Title: Am J Cancer Res Volume: 2 Pages: 459-475
  • Peer Reviewed
• [Journal Article] Notch receptor inhibition reveals the importance of cyclin D1 and Wnt signaling in invasive esophageal squamous cell carcinoma. (2012)
  • Author(s): Naganuma S, Whelan KA, Natsuizaka M, Kagawa S, Kinugasa H, Chang S, Subramanian H, Rhoades B, Ohashi S, Itoh H, Herlyn M, Diehl JA,Gimotty PA, Klein-Szanto AJ, Nakagawa H.
  • Journal Title: Am J Cancer Res Volume: 2 Pages: 459-475
  • Peer Reviewed
• [Journal Article] Regional quantification of muscarinic acetylcholine receptors and β-adrenoceptors in human airways (2012)
  • Author(s): Ikeda T, Anisuzzaman AS, Yoshiki H, Sasaki M, Koshiji T, Uwada J, Nishimune A, Itoh H, Muramatsu I
  • Journal Title: Br J Pharmacol Volume: (In press) Pages: 1804-1814
  • DOI: 10.1111/j.1476-5381.2012.01881.x
  • Peer Reviewed
• [Journal Article] Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma (2011)
  • Author(s): Dai Susuki, Sotai Kimura, Seiji Naganuma, Katsuki Tsuchiyama, Toshiaki Tanaka, Naomi Kitamura, Shigeharu Fujieda, Hiroshi Itoh
  • Journal Title: Cancer Sci Volume: 102(12) Pages: 2164-2171
  • DOI: 10.1111/j.1349-7006.2011.02096.x
  • NAID: 10030245835
  • Peer Reviewed
• [Journal Article] A NOTCH3-mediated squamous cell differentiation program limits expansion of EMT-competent cells that express the ZEB transcription factors (2011)
  • Author(s): Ohashi S, Natsuizaka M, Naganuma S, Kagawa S, Kimura S, Itoh H, Kaiman RA, Nakagawa M, Darling DS, Basu D, Gimotty PA, Klein-Szanto AJ, Diehl JA, Herlyn M, Nakagawa H
  • Journal Title: Cancer Res Volume: 71 Pages: 6836-6847
  • DOI: 10.1158/0008-5472.can-11-0846
  • Peer Reviewed
• [Journal Article] A NOTCH3-mediated squamous cell differentiation program limits expansion of EMT-competent cells that express the ZEB transcription factors. (2011)
  • Author(s): Ohashi S, Natsuizaka M, Naganuma S, Kagawa S, Kimura S, Itoh H, Kaiman RA, Nakagawa M, Darling DS, Basu D, Gimotty PA, Klein-Szanto AJ, Diehl JA, Herlyn M, Nakagawa H
  • Journal Title: Cancer Res Volume: 71 Pages: 6836-6847
• [Presentation] 腹膜偽粘液腫の病理学的検討 (2014)
  • Author(s): 高原千咲, 濱崎太郎, 小賀厚徳, 伊藤秀明, 伊藤浩史
  • Organizer: 第１０３回日本病理学会総会
  • Place of Presentation: 広島（広島国際会議場）
• [Presentation] 子宮頚部腺癌におけるp16発現とh-HPV感染の検討 (2014)
  • Author(s): 名尾知絵, 河内茂人, 池本健三, 伊藤秀明, 奥田信一郎, 伊藤浩史
  • Organizer: 第１０３回日本病理学会総会
  • Place of Presentation: 広島（広島国際会議場）
• [Presentation] Comparison of proliferative capability of neoplastic and non-neoplastic cells by image cytometry (2013)
  • Author(s): Ito H, Oga A, Ikemoto K, Kondo T, Chochi Y, Kawauchi S, Sasaki K, Itoh H
  • Organizer: 第72回日本癌学会総会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2013-10-03
• [Presentation] Comparison of proliferative capability of neoplastic and non-neoplastic cells by image cytometry (2013)
  • Author(s): Hideaki Ito, Atsunori Oga,Kenzo Ikemoto, Tomoko Kondo, Yasuyo Chochi, Shigeto Kawauchi, Kohsuke Sasaki, Hiroshi Itoh
  • Organizer: 第７２回日本癌学会総会
  • Place of Presentation: 横浜（パシフィコ横浜）
• [Presentation] The inhibitory effect of autophagy induced by mTOR inhibitor on cell proliferation in renal cell carcinoma (2012)
  • Author(s): Taga M, Ito H, Taga M, Inamura S, Tsuchiyama K, Naganuma S, Yakoyama O, Itoh H
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(福岡国際会議場)
  • Year and Date: 2012-12-11
• [Presentation] A genetic crosstalk with Notch signaling in an organotypic 3D culture model of squamous cell carcinoma (2012)
  • Author(s): Naganuma S, Natsuizaka M, Ohashi S, Nakagawa H, Itoh H
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(福岡国際会議場)
  • Year and Date: 2012-12-11
• [Presentation] Expression of microRNAs associated with Gleason grading system in prostate cancer (2012)
  • Author(s): Tsuchiyama K, Ito H, Taga M, Naganuma S, Yakoyama O, Itoh H
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: 札幌(ロイトン札幌)
  • Year and Date: 2012-09-19
• [Presentation] Relationship between microRNA expression and Gleason grading system in prostate cancer (2012)
  • Author(s): Tsuchiyama K, Ito H, Taga M, Oshinoya K, Nagano K, Yokoyama O, Itoh H
  • Organizer: 103rd Annual meeting, American Association for Cancer Research (AACR)
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2012-04-04
• [Presentation] Expression of microRNAs associated with Gleason grading system in prostate cancer. (2012)
  • Author(s): Tsuchiyama K, Ito H, Taga M, Naganuma S, Yokoyama O, Itoh H.
  • Organizer: 第７１回日本癌学会総会
  • Place of Presentation: 札幌（ロイトン札幌）
• [Presentation] The inhibitory effect of autophagy induced by mTOR inhibitor on cell proliferation in renal cell carcinoma (2012)
  • Author(s): Minekatsu Taga, Hideaki Ito, So Inamura, Katsuki Tsuchiyama, Seiji Naganuma, Osamu Yokoyama, Hiroshi Itoh
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡（福岡国際会議場）
• [Presentation] A genetic crosstalk with Notch signaling in an organotypic 3D culture model of squamous cell carcinoma (2012)
  • Author(s): Seiji Naganuma, Mitsuteru Natsuizaka, Shinya Ohashi, Hiroshi Nakagawa, Hiroshi Itoh, et al
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡（福岡国際会議場）
• [Presentation] 前立腺癌におけるGleason grading systemと相関するmicroRNAの発現解析 (2011)
  • Author(s): 土山克樹,伊藤秀明,多賀峰克,押野谷幸之助,長野賢一,伊藤浩史
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2011-12-14
• [Presentation] ヒト頭頚部扁平上皮癌における肝細胞増殖因子によるマイクロRNA発現調節 (2011)
  • Author(s): 鈴木弟,土山克樹,木村相泰,長沼誠二,藤枝重治,喜多村直実,伊藤浩史
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2011-12-14
• [Presentation] 前立腺癌のGleason grading systemと関連するmicroRNAの発現解析 (2011)
  • Author(s): 土山克樹,伊藤秀明,多賀峰克,伊藤浩史
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋(名古屋国際会議場)
  • Year and Date: 2011-10-04
• [Presentation] Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma (2011)
  • Author(s): Susuki D, Kimura S, Naganuma S, Tsuchiyama K, Tanaka T, Kitamura N, Fujieda S, Itoh H
  • Organizer: 102nd Annual meeting, American Association for Cancer Research (AACR)
  • Place of Presentation: Orlando, FL, USA
  • Year and Date: 2011-04-03
• [Presentation] Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma. (2011)
  • Author(s): Susuki D, Kimura S, Naganuma S, Tsuchiyama K, Tanaka T, Kitamura N, Fujieda S, Itoh H
  • Organizer: 102nd Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Orlando FL, USA
• [Presentation] 前立腺癌のGleason grading systemと関連するmicroRNAの発現解析 (2011)
  • Author(s): 土山克樹, 伊藤秀明, 多賀峰克, 伊藤浩史
  • Organizer: 第７０回日本癌学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] 前立腺癌におけるGleason grading systemと相関するmicroRNAの発現解析 (2011)
  • Author(s): 土山克樹, 伊藤秀明, 多賀峰克, 押野谷幸之助, 長野賢一, 伊藤浩史
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ヒト頭頸部扁平上皮癌における肝細胞増殖因子によるマイクロRNA発現調節 (2011)
  • Author(s): 鈴木弟, 土山克樹, 木村相泰, 長沼誠二, 藤枝重治, 喜多村直実, 伊藤浩史
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Relationship between microRNA expression and Gleason grading system in prostate cancer
  • Author(s): Katsuki Tsuchiyama, Hideaki Ito, Minekatsu Taga, Konosuke Oshinoya, Kenichi Nagano, Osamu Yokoyama, Hiroshi Itoh
  • Organizer: 102nd Annual Meeting of American Association of Cancer Research (AACR)
  • Place of Presentation: Chicago, IL, USA
• [Remarks]
  • URL: http://ds.cc.yamaguchi-u.ac.jp/~2byouri/index.html