Identification of novel causative genes in mesothelioma and the elucidation of the carcinogenic mechanism

Research Project

Project/Area Number	23590464
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Experimental pathology
Research Institution	Shimane University
Principal Investigator	KAKAZU Naoki 島根大学, 医学部, 准教授 (20264757)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	中皮腫 / 融合転写産物 / 悪性中皮腫 / 融合遺伝子
Outline of Final Research Achievements	Mesothelioma is a poor prognosis tumor due to former exposure to asbestos. We analyzed mesothelioma cells by next generation sequencing to identify novel candidate fusion genes associated with the development of mesothelioma. Firstly, we performed whole transcriptome shotgun sequencing (RNA-Seq) of a mesothelioma cell line and comprehensively detected many fusion transcripts. Secondly, we performed RT-PCR in the cell line and the other five to confirm the presence of these detected fusion transcripts, and narrowed down the candidate fusion genes. These results suggest that two fusion genes (DUS4L-BCAP29, PDPN-PRDM2) are the potential candidates implicated in the pathogenesis of mesothelioma in this study.