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Analysis of beta-Catenin/Sox2/p63 Signal Pathway in Lung Epithelial Differentiation and Tumorigenesis

Research Project

Project/Area Number 23590466
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionKumamoto University

Principal Investigator

HASHIMOTO Shuichi  熊本大学, 大学院生命科学研究部(医), 准教授 (00243931)

Co-Investigator(Renkei-kenkyūsha) ITO Takaaki  熊本大学, 大学院生命科学研究部, 教授 (70168392)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
KeywordsLung / Cancer / Epithelium / Differentiation / Stem Cell / beta-catenin / Sox2 / p63 / Sox9 / Notch / Stem cell / Airway epithelium
Research Abstract

We clarified the expression of Wnt/beta-catenin signaling and related factors in lung cancers and the regulatory mechanisms between beta-catenin and Sox2 expression. Sox2 was highly expressed in small and squamous cell carcinomas, Sox9 in squamous but also in adeno and large cell carcinomas, p63 in squamous cell carcinomas, and Notch1 especially in adenocarcinomas. These data suggests their own functions in tumorigenesis or tumor progression in each lung cancer subtype. Next we established the 3D culture system of human bronchial basal cells (VA10). Using this system we clarified that BIO (GSK3 inhibitor) contributed to form cell polarity and co-stimulation of Wnt3a and EGF induced distinct extension of cells from the sphere colonies suggesting the function to accelerate cell cycling or cell division. ChIP analysis revealed that beta-catenin might not directly bind the Sox2 promoter region and downregulate Sox2 expression in other indirect way.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2013

All Journal Article (4 results)

  • [Journal Article] 免疫組織化学的染色法;講座研究手法入門:生化学的・免疫学的実験法2013

    • Author(s)
      橋本修一、中川和憲、Barry R Stripp
    • Journal Title

      呼吸

      Volume: 32巻12号 Pages: 1162-1175

    • Related Report
      2013 Final Research Report
  • [Journal Article] 発達肺および傷害再生肺における肺上皮分化制御機構-肺幹細胞生物学の進歩と肺再生医療への礎–日本肺サーファクタント2013

    • Author(s)
      橋本修一
    • Journal Title

      界面医学会雑誌

      Volume: 44巻 Pages: 2-15

    • Related Report
      2013 Final Research Report
  • [Journal Article] 発達肺および傷害再生肺における肺上皮分化制御機構 - 肺幹細胞生物学の進歩と肺再生医療への礎 -2013

    • Author(s)
      橋本 修一
    • Journal Title

      日本肺サーファクタント・界面医学会雑誌

      Volume: 44 Pages: 2-15

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 免疫組織化学的染色法2013

    • Author(s)
      橋本 修一、中川 和憲、Barry R. Stripp
    • Journal Title

      呼吸

      Volume: 32 Pages: 1162-1175

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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