Analysis of tumor suppressive effects of miRNA using Dicer-deficient mice

• Project/Area Number: 23590482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: SEKINE Shigeki 独立行政法人国立がん研究センター, 中央病院, 医長 (10321879)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 肝発がん / Dicer / miRNA / 肝細胞がん

Research Abstract

We analyzed hepatocellular carcinomas arising in hepatocyte-specific Dicer-deficient mice and found that the altered expression of a number of cancer-related genes. However, we could not identify genes that are commonly dysregulated or mutated in multiple tumors. To analyze the role of Dicer in hepatocarcinogenesis, we established a mouse model that allows the induction of liver tumorigenesis by gene transfer in vivo.

Research Products

• [Journal Article] Bile Acids Increase Levels of microRNAs 221 and 222, Leading to Degradation of CDX2 During Esophageal Carcinogenesis (2013)
  • Author(s): J. Matsuzaki, H. Suzuki, H. Tsugawa, M. Watanabe, S. Hossain, T. Akaike, T. Hibi, et. al.
  • Journal Title: Gastroenterology Volume: (In Press) Issue: 6 Pages: 1300-1311
  • DOI: 10.1053/j.gastro.2013.08.008
  • Peer Reviewed
• [Journal Article] Soluble interleukin-6 receptor is a serum biomarker for the response of esophageal carcinoma to neoadjuvant chemoradiotherapy. (2013)
  • Author(s): Makuuchi Y, Honda K, Osaka Y, Kato K, Kojima T, Daiko H, Igaki H, Ito Y, Hoshino S, Tachibana S, Watanabe T, Furuta K, Sekine S, Umaki T, Watabe Y, Miura N, Ono M, Tsuchida A, Yamada T.
  • Journal Title: Cancer Sci. Volume: Aug;104(8): Issue: 8 Pages: 1045-51
  • DOI: 10.1111/cas.12187
  • Peer Reviewed
• [Journal Article] Frequent GNAS and KRAS mutations in pyloric gland adenoma of the stomach and duodenum. (2013)
  • Author(s): Matsubara A,
  • Journal Title: J Pathol. Volume: 229 Issue: 4 Pages: 579-587
  • DOI: 10.1002/path.4153
  • Peer Reviewed
• [Journal Article] Overexpression ofα-methylacyl-CoA racemase is associated with CTNNB1 mutations in hepatocellular carcinomas (2011)
  • Author(s): Sekine S, Ogawa R, Ojima H, Kanai Y
  • Journal Title: Histopathology Volume: 58(5) Issue: 5 Pages: 712-9
  • DOI: 10.1111/j.1365-2559.2011.03798.x
  • Peer Reviewed
• [Journal Article] Expression of SLCO1B3 is associated with intratumoral cholestasis and CTNNB1 mutations in hepatocellular carcinoma (2011)
  • Author(s): Sekine S, Ogawa R, Ojima H, Kanai Y
  • Journal Title: Cancer Sci Volume: 102(9) Issue: 9 Pages: 1742-7
  • DOI: 10.1111/j.1349-7006.2011.01990.x
  • NAID: 10030243397
  • Peer Reviewed
• [Journal Article] Hepatomas with activating Ctnnb1 mutations in 'Ctnnb1-deficient' livers : a tricky aspect of a conditional knockout mouse model (2011)
  • Author(s): Sekine S, Ogawa R, Kanai Y
  • Journal Title: Carcinogenesis Volume: 32(4) Issue: 4 Pages: 622-8
  • DOI: 10.1093/carcin/bgr002
  • Peer Reviewed