Molecular mechanism of miR-143/145 down-regulation in cancer and disease

Research Project

Project/Area Number	23590483
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Experimental pathology
Research Institution	Institute for Developmental Research, Aichi Human Service Center (2012-2013) Gifu International Institute of Biotechnology (2011)
Principal Investigator	IIO Akio 愛知県心身障害者コロニー発達障害研究所, 発生障害学部, リサーチレジデント (80344349)
Co-Investigator(Renkei-kenkyūsha)	AKAO Yukihiro 岐阜大学院, 連合創薬, 教授 (00222505)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	microRNA / 癌 / プロモーター / DDX6 / P-body
Research Abstract	In this study, we found that NCR143/145 promoter was feed-back regulated by SRF/ELK1 and its regulatory mechanisms were disrupted in cancers. Furthermore, we showed that DDX6 and AGO2 promoted the nuclear export and degradation of NCR143/145 RNA through decapping followed by miR-143/145 down-regulation.