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Animal model for the treatment of Chronic Chagas Disease using the combination of drug and vaccine

Research Project

Project/Area Number 23590486
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

HIRAYAMA Kenji  長崎大学, 熱帯医学研究所, 教授 (60189868)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Mihoko  長崎大学, 熱帯医学研究所, 講師 (40336186)
MOHAMMED NASIR Shuaibu  長崎大学, 熱帯医学研究所, 助教 (30535745)
YANAGI Tetsuo  長崎大学, 熱帯医学研究所, 助手 (10174541)
UEMURA Haruki  長崎大学, 熱帯医学研究所, 講師 (60184975)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsシャーガス病 / ワクチン / 慢性感染症 / ベンズニダゾール / 遺伝子導入原虫 / 画像解析 / DNAワクチン / ナノテクノロジー / クルーズトリパノソーマ / マウスモデル
Research Abstract

To find a new strategy to treat a chronic Chagas adult patient, we tried to establish a chronic infection model with mouse and luciferase gene transfected Y strain of Trypanosoma cruzi and successfully obtain a protocol to observe the chronic status using whole body imaging system. Then we prepared the DNA vaccine using previously reported three candidate genes namely TcG1, TcG2 and Tcg4 and our recently established pVAX 200DEST nano-particle adjuvant to observe that effect on the elimination of chronically infected parasites. The evaluation of the vaccines has not yet finished but the vaccination could provoke substantial immunological effect by B and T cell levels.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (11 results) (of which Invited: 2 results) Book (8 results) Remarks (1 results)

  • [Journal Article] 21-Hydroxylase gene mutant allele CYP21A2 15 strongly linked to the resistant HLA haplotype B 14:02-DRB1 01:02 in chronic Chagas disease.2013

    • Author(s)
      Del Puerto F, Kikuchi M, Nishizawa JE, Roca Y, Avilas C, Gianella A, Lora J, Gutierrez Velarde FU, Hirayama K
    • Journal Title

      Hum Immunol.

      Volume: 74(6) Issue: 6 Pages: 783-786

    • DOI

      10.1016/j.humimm.2013.01.023

    • NAID

      120006985861

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 21-Hydroxylase gene mutant allele CYP21A2(∗)15 strongly linked to the resistant HLA haplotype B(∗)14:02-DRB1(∗)01:02 in chronic Chagas disease.2013

    • Author(s)
      Del Puerto F, Kikuchi M, Nishizawa JE, Roca Y, Avilas C, Gianella A, Lora J, Gutierrez Velarde FU, Hirayama K.
    • Journal Title

      Hum Immunol

      Volume: 74(6) Pages: 783-6

    • NAID

      120006985861

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protective human leucocyte antigen haplotype, HLA-DRB1 01-B 14, against chronic Chagas disease in Bolivia2012

    • Author(s)
      Del Puerto F, Nishizawa JE, Kikuchi M, 他8人, Hirayama K
    • Journal Title

      PLoS Negl Trop Dis

      Volume: 6(3) Issue: 3 Pages: e1587-e1587

    • DOI

      10.1371/journal.pntd.0001587

    • NAID

      120006985415

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] GENETIC ANALYSIS OF CHRONIC CHAGAS DISEASE IN BOLIVIA -HOST OR PARASITE FACTOR?2013

    • Author(s)
      Kenji Hirayama
    • Organizer
      XLIX CONGRESS OF THE BRAZILIAN SOCIETY OF TROPICAL MEDICINE
    • Place of Presentation
      the Centro de Convenções Arquiteto Rubens Gil de Camilo (第4回ブラジル熱帯医学会年次大会招待講演 2013年8月8日Campo Grande, MS, Brazil, ブラジル、カンポグランデ市)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 淘汰圧としての熱帯感染症2012

    • Author(s)
      平山謙二
    • Organizer
      シンポジウム7感染症の遺伝学:ゲノムと環境の相互作用日本人類遺伝学会第57回大会
    • Place of Presentation
      京王プラザホテル、東京都(日本人類遺伝学会第57回大会解析から応用へ、そして未来への飛躍プログラム、pp110、2012)
    • Related Report
      2013 Final Research Report
  • [Presentation] V281L of the CYP21 gene causing 21-Hydroxylase deficiency located in the class III region of resistant HLA haplotype in the chronic Chagas disease2012

    • Author(s)
      Florencia Del Puerto Rodas, Eiki J. Nishizawa,菊池三穂子, Yelin Roca, Luis A. Renjel,小宮憲洋、前村浩二、平山謙二
    • Organizer
      第21回日本組織適合性学会大会
    • Place of Presentation
      明治大学駿河台キャンパスリバティホール、東京(日本組織適合性学会誌MHC Vol. 19 No. 2, 2012、pp106, 2012)
    • Related Report
      2013 Final Research Report
  • [Presentation] 熱帯感染症とHLA2012

    • Author(s)
      平山謙二
    • Organizer
      第21回日本組織適合性学会大会
    • Place of Presentation
      明治大学駿河台キャンパスリバティホール、東京(日本組織適合性学会誌MHC Vol. 19 No. 2, 2012、pp61, 2012)
    • Related Report
      2013 Final Research Report
  • [Presentation] V281L of the CYP21 gene causing 21-Hydroxylase deficiency located in the class III region of resistant HLA haplotype in the chronic Chagas disease.2012

    • Author(s)
      Florencia Del Puerto Rodas, Eiki J. Nishizawa, 菊池三穂子, Yelin Roca, Luis A. Renjel, 小宮憲洋、前村浩二、平山謙二:
    • Organizer
      第21回日本組織適合性学会大会
    • Place of Presentation
      明治大学駿河台キャンパス リバティホール、東京
    • Related Report
      2012 Research-status Report
  • [Presentation] del Puerto Rodas, Kenji Hirayama et al. No Correlation Between Trypanosoma cruzi Level and Clinical Manifestation in a Cross Sectional Study on Chronic Patients in Bolivia2011

    • Author(s)
      Ramona F
    • Organizer
      60th ASTMH Annual Meeting
    • Place of Presentation
      Philadelphia Marriott Downtown, Philadelphia , PA, USA. (Am J Trop Med Hyg, Vol. 85 Dec 2011 Number 6 Supplement; American Society of Tropical Medicine and Hygiene 60th Annual Meeting Program : LB2276)
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunogenetic analysis of chronic Chagas disease in Bolivia2011

    • Author(s)
      Florencia del Puerto Rodas, Juan Eiki Nishizawa, Mihoko Kikuchi, Naomi Iihoshi, Yelin Raca, Cinthia Avilas, Alberto Gianella, Javier Lora, Freddy Udalrico. Gutierrez. Velarde, Luis Alberto. Renjel, Sachio Miura, Hiroo Higo, Norihiro Komita. Kouji Maemura, Michio Yasunami, and Kenji Hirayama
    • Organizer
      第20回日本組織適合性学会大会
    • Place of Presentation
      ツインメッセ静岡北館および中央棟、静岡日本組織適合性学会誌MHC、Vol. 18 No. 2, 2011、pp152. 2011)
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantification of Trypanosoma cruzi parasitemia in peripheral blood of Chronic Chagasic patients in Bolivia using real time PCR2011

    • Author(s)
      Puerto Florencia del、Nishizawa Juan Eiki、菊池三穂子、Iihoshi Naomi、Roca Yelin、Avilas Cinthia、Gianella Alberto、Lora Javier、Velarde Freddy Udalrico Gutierrez、Renjel Luis Alberto、三浦左千夫、肥後広雄、小宮憲洋、前村浩二、安波道郎、平山謙二
    • Organizer
      第80回日本寄生虫学会大会
    • Place of Presentation
      第80回日本寄生虫学会大会第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会プログラム・抄録集、pp72. 2011)
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantification of Trypanosoma cruzi parasitemia in peripheral blood of Chronic Chagasic patients in Bolivia using real time PCR .2011

    • Author(s)
      Puerto F D, Nishizawa J E, Kikuchi M、 Iihoshi N、 Roca Y、 Avilas C、 Gianella A、 Lora J、 Velarde F U G、 Renjel L A、 Miura S, Higo H, Komiya N, Maemura K、Yasunami M, Hirayama K
    • Organizer
      第80回日本寄生虫学会大会
    • Place of Presentation
      東京慈恵会医科大学、 東京、
    • Related Report
      2011 Research-status Report
  • [Presentation] Immunogenetic analysis of chronic Chagas disease in Bolivia2011

    • Author(s)
      F del Puerto Rodas, J E Nishizawa, M Kikuchi, N Iihoshi, Y Raca, C Avilas, A G, J Lora, F U. G. Velarde, L A. Renjel, S Miura, H Higo, N Komita. K Maemura, M Yasunami & K Hirayama
    • Organizer
      第20回日本組織適合性学会大会
    • Place of Presentation
      ツインメッセ静岡、 静岡、
    • Related Report
      2011 Research-status Report
  • [Presentation] GENETIC ANALYSIS OF CHRONIC CHAGAS DISEASE IN BOLIVIA -HOST OR PARASITE FACTOR?

    • Author(s)
      Kenji Hirayama
    • Organizer
      XLIX CONGRESS OF THE BRAZILIAN SOCIETY OF TROPICAL MEDICINE, ブラジル熱帯医学会年次大会招待講演
    • Place of Presentation
      Campo Grande, MS, Brazil, ブラジル、カンポグランデ市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 医動物学臨床検査学第2版2014

    • Author(s)
      藤田紘一郎,平山謙二著
    • Publisher
      医歯薬学出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] コメント,心疾患や消化器疾患の原因が寄生原虫だった!?,トレンドビュー◎シャーガス病を知っていますか?2013

    • Author(s)
      平山謙二
    • Publisher
      日経メディカルオンライン
    • Related Report
      2013 Final Research Report
  • [Book] 化学療法の領域、Vol.29、No.8, 5.シャーガス病,特集世界に広がるトロピカルディジージズ2013

    • Author(s)
      平山謙二
    • Publisher
      株式会社医薬ジャーナル社
    • Related Report
      2013 Final Research Report
  • [Book] 5.シャーガス病, 特集 世界に広がるトロピカルディジージズ、化学療法の領域Vol.29、No.8、pp60-682013

    • Author(s)
      平山謙二
    • Total Pages
      9
    • Publisher
      医薬ジャーナル社
    • Related Report
      2013 Annual Research Report
  • [Book] 日経メディカルオンライン2013

    • Author(s)
      平山謙二
    • Total Pages
      3
    • Publisher
      日本経済新聞社
    • Related Report
      2013 Annual Research Report
  • [Book] 臨床検査学講座第2版医動物学2012

    • Author(s)
      藤田紘一郎,平山謙二著者
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] 熱帯医学と感染症,感染症事典2012

    • Author(s)
      平山謙二, (編集;感染症事典編集委員会)
    • Publisher
      医学書院
    • Related Report
      2013 Final Research Report
  • [Book] 感染症事典, 感染症とは2012

    • Author(s)
      平山謙二, (編集;感染症事典編集委員会)
    • Publisher
      医学書院
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.tm.nagasaki-u.ac.jp/hiraken/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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