Th2 independent NB expulsion by natural helper cells

• Project/Area Number: 23590488
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: HONMA Kiri 長崎大学, 医歯(薬)学総合研究科, 准教授 (70307940)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Th2 / 自然リンパ球 / 蠕虫 / 腸管免疫 / サイトカイン / 免疫 / IRF-4 / 転写因子 / NH細胞 / IRF4 / ヘルパーT細胞

Research Abstract

Interferon regulatory factor 4 (IRF4) is the development of Th2. In IRF4-deficient mice (IRF4 KO), Th2 development does not occur. Nippostrongyrus brasiliensis (NB) is one of parasite and is eliminated by antigen-specific Th2, which is generated during infection. However, Th2 independent NB elimination was found in IRF4 KO. This study indicated that group2 innate lymphocytes (ILC2) played the crucial role in Th2 independent NB expulsion and IRF4 directly regulated the Th2 cytokine production, such as IL5 or IL13 although IRF4 was dispensable of ILC2 development.

Research Products

• [Journal Article] IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major (2014)
  • Author(s): Akbari Masoud, Kiri Honma, Daisuke Kimura, Mana Miyakoda, Kazumi Kimura, Toshifumi Matsuyama, Katsuyuki Yui
  • Journal Title: J. Immuno. Volume: 192 Pages: 2271-2279
  • Peer Reviewed
• [Journal Article] RF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major. (2014)
  • Author(s): Akbari Masoud, Kiri Honma, Daisuke Kimura, Mana Miyakoda, Kazumi Kimura, Toshifumi Matsuyama, Katsuyuki Yui
  • Journal Title: Journal of Immunology Volume: 192 Issue: 5 Pages: 2271-2279
  • DOI: 10.4049/jimmunol.1301914
  • Peer Reviewed
• [Journal Article] CD8^+ T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection (2013)
  • Author(s): Kazumi Kimura, Daisuke Kimura, Yoshifumi Matsushima, Mana Miyakoda, Kiri Honma, Masao Yuda, and Katsuyuki Yui
  • Journal Title: Infection and Immunity Volume: 81 Pages: 3825-3834
  • NAID: 120006986085
  • Peer Reviewed
• [Journal Article] Smad2/3 and IRF4 Play a Cooperative Role in IL-9–Producing T Cell Induction (2013)
  • Author(s): Taiga Tamiya, Kenji Ichiyama, Hitoshi Kotani, Tomohiro Fukaya, Takashi Sekiya, Takashi Shichita, Kiri Honma, Katsuyuki Yui, Toshifumi Matsuyama, Takako Nakao, Satoru Fukuyama, Hiromasa Inoue, Masatoshi Nomura, Akihiko Yoshimura
  • Journal Title: J. Immunol. Volume: 191 Pages: 2360-2371
  • Peer Reviewed
• [Journal Article] Accumulation of major histocompatibility complex class II.CD11c non‐lymphoid cells in the spleen during infection with Plasmodium yoelii is lymphocyte‐dependent (2013)
  • Author(s): Rika Kamei , Mana Miyakoda, Takahiko Tamura, Daisuke Kimura, Kiri Honma, Kazumi Kimura, Katsuyuki Yui
  • Journal Title: Microbiology and Immunology Volume: 57 Pages: 213-223
  • Peer Reviewed
• [Journal Article] CD19 (+) B cells Confer Protection Against Experimental Cerebral Malaria In Semi-immune Rodent Model (2013)
  • Author(s): Lam Quoc Bao, Nguyen Tien Huy, Mihoko Kikuchi, Tetsuo Yanagi, Masachika Senba, Mohammed Nasir Shuaibu, Kiri Honma, Katsuyuki Yui, Kenji Hirayama
  • Journal Title: PLosOne Volume: 8
  • NAID: 120006986217
  • Peer Reviewed
• [Journal Article] CD8+ T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection. (2013)
  • Author(s): Kazumi Kimura, Daisuke Kimura, Yoshifumi Matsushima, Mana Miyakoda, Kiri Honma, Masao Yuda, and Katsuyuki Yui
  • Journal Title: Infection of Immunology Volume: 81 Issue: 10 Pages: 3825-3834
  • DOI: 10.1128/iai.00570-13
  • NAID: 120006986085
  • Peer Reviewed
• [Journal Article] Smad2/3 and IRF4 play a cooperative role in IL-9-producing T cell induction. (2013)
  • Author(s): Tamiya T, Ichiyama K, Kotani H, Fukaya T, Sekiya T, et al.
  • Journal Title: J Immunol. Volume: 191 Issue: 5 Pages: 2360-2371
  • DOI: 10.4049/jimmunol.1301276
  • Peer Reviewed
• [Journal Article] Accumulation of MHC class II+ CD11c- non-lymphoid cells in the spleen during infection with Plasmodium yoelii is lymphocyte-dependent. (2013)
  • Author(s): Kamei R, Miyakoda M, Tamura T, Kimura D, Honma K, Kimura K, Yui K,
  • Journal Title: Microbiology and Immunology Volume: 57 Issue: 3 Pages: 213-223
  • DOI: 10.1111/1348-0421.12024
  • Peer Reviewed
• [Journal Article] CD19 (+) B cells Confer Protection Against Experimental Cerebral Malaria In Semi-immune Rodent Model. (2013)
  • Author(s): Lam Quoc Bao, Nguyen Tien Huy, Mihoko Kikuchi, Tetsuo Yanagi, Masachika Senba, Mohammed Nasir Shuaibu, Kiri Honma, Katsuyuki Yui, Kenji Hirayama
  • Journal Title: PLos One Volume: 8 Issue: 5 Pages: e64836-e64836
  • DOI: 10.1371/journal.pone.0064836
  • NAID: 120006986217
  • Peer Reviewed
• [Journal Article] Development of memory CD8^+ T cells and their recall responses during blood-stage infection with Plasmodium berghei ANKA (2012)
  • Author(s): Mana Miyakoda, Daisuke Kimura, Kiri Honma, Kazumi Kimura, Masao Yuda, Katsuyuki Yui
  • Journal Title: Journal of Immunology Volume: 189 Pages: 4396-4404
  • Peer Reviewed
• [Journal Article] Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel (2011)
  • Author(s): Shindo H., Yasui K., Yamamoto K., Honma K., Yui K., Kohno T., Yuhua Ma Y., Jiew CK., Kubo Y., Aihara H., Ito T., Nagayasu T., Matsuyama T., Hayashi H.
  • Journal Title: Cytokine Volume: 56 Pages: 564-572
  • NAID: 120006985203
  • Peer Reviewed
• [Presentation] IRF4 in dendritic cells regulates protective immunity against infection with Leishmania major (2014)
  • Author(s): Akbari Masoud, 本間季里、木村大輔、都田真奈、木村一美、松山俊文、由井克之
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 松山市 愛媛大学城北キャンパス
• [Presentation] マラリア原虫特異的CD4+ T細胞は、IL-27を介してCD4+ T細胞の防御免疫を抑制する (2014)
  • Author(s): 木村大輔、都田真奈、木村一美、本間季里、原博満、堀昌平、吉田裕樹、由井克之
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 松山市 愛媛大学城北キャンパス
• [Presentation] IL-27 is produced by CD4+ T cells during infection with malaria parasites and inhibits protective immune responses (2013)
  • Author(s): KIMURA Daisuke, MIYAKODA Mana, KIMURA Kazumi, HONMA Kiri, HARA Hiromitsu, HORI Shohei, YOSHIDA Hiroki, YUI Katsuyuki
  • Organizer: 第42回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録)
• [Presentation] IFN-γ-receptor-1 is expressed abundantly on splenic B cells of lupus-prone MRL/lpr mice and is involved in peripheral B-cell survival/maintenance and autoantibody production (2013)
  • Author(s): Takeshi Machida, Natsumi Sakamoto, Kiri Honma, Toshifumi Matsuyama, Katsuyuki Yui, Hideharu Sekine
  • Organizer: 第42回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録)
• [Presentation] IL-27 is produced by CD4+ T cells during infection with malaria parasites and inhibits protective immune responses. (2013)
  • Author(s): KIMURA Daisuke, MIYAKODA Mana, KIMURA Kazumi, HONMA Kiri, HARA Hiromitsu, HORI Shohei, YOSHIDA Hiroki, YUI Katsuyuki
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市 幕張メッセ
• [Presentation] IRF4 is critical for proliferation and effector differentation of CD8＋ T cells. (2013)
  • Author(s): Mana Miyakoda, Kiri Honma, Daisuke Kimura, Kazumi Kimura, Toshifumi Matsuyama, Katsuyuki Yui
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市 幕張メッセ
• [Presentation] IFN-γ-receptor-1 is expressed abundantly on splenic B cells of lupus-prone MRL/lpr mice and is involved in peripheral B-cell survival/maintenance and autoantibody production (2013)
  • Author(s): Takeshi Machida, Natsumi Sakamoto, Kiri Honma, Toshifumi Matsuyama, Katsuyuki Yui, Hideharu Sekine
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉市 幕張メッセ
• [Presentation] マラリア原虫特異的CD4T細胞はEBI3陽性サイトカイン介して他のCD4T細胞のIL-2産生を抑制する (2013)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、原博満、吉田裕樹、由井克之
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] マラリア原虫感染における抗原特異的記憶CD8+ T細胞の二次応答抑制 (2013)
  • Author(s): 都田真奈、木村大輔、本間季里、木村一美、油田正夫、由井克之
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] The role of IRF4 expressed in DCs during protective immune responses against Leishmania major (2012)
  • Author(s): Masoud Akbari, Kiri Honma, Daisuke Kiimura, Mana Miyakoda, Kazumi Kimura, Toshifumi Matsuyama, Katsuyuki Yui
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.75)
• [Presentation] Th2細胞非依存的N. brasiliensis感染排除機構の解析—ナチュラルヘルパー細胞の解析 (2012)
  • Author(s): 本間季里、木村大輔、木村一美、都田真奈、茂呂和世、小安重夫、松山俊文、由井克之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.71)
• [Presentation] マラリア原虫感染によって引き起こされるCD4+ T細胞のIL-2産生抑制機序の解析 (2012)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、原博満、吉田裕樹、由井克之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.74)
• [Presentation] The critical role of IRF4 expressed in skin dendritic cells for the induction of protective immune responses against infection with Leishmania major (2012)
  • Author(s): Masoud Akbari, Kiri Honma, Daisuke Kimura, Mana Miyakoda, Takahiko Tamura, Kazumi Kimura, Toshifumi Matsuyama, Ulf Klein, Katsuyuki Yui
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.58)
• [Presentation] マラリア原虫感染中にみられる抗原特異的記憶CD8^+ T細胞の二次応答の抑制 (2012)
  • Author(s): 都田真奈、木村大輔、本間季里、木村一美、油田正夫、由井克之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.74)
• [Presentation] マラリア肝細胞期における抗原提示機構の解析 (2012)
  • Author(s): 木村一美、木村大輔、都田真奈、本間季里、田村隆彦、油田正夫、由井克之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.75)
• [Presentation] マラリア原虫特異的CD4T細胞はEBI3陽性サイトカイン介して他のCD4T細胞のIL-2産生を抑制する (2012)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、原博満、吉田裕樹、由井克之
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 神戸(p.75)
• [Presentation] Negative regulation of Th2-type cytokine production in natural helper cells by IRF4 (2012)
  • Author(s): Kiri Honma, Daisuke Kimura, Kazumi Kimura, Mana Miyakoda, Kazuyo Moro, Shigeo Koyasu, Toshifumi Matsuyama, Katsuyuki Yui
  • Organizer: 日本免疫学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] CD4 T cells produce EBI-3+ cytokine inhibiting their own protective immune responses during infection with malaria parasites. (2012)
  • Author(s): KIMURA Daisuke, MIYAKODA Mana, HONMA Kiri, KIMURA Kazumi, HARA Hiromitsu, YOSHIDA Hiroki, YUI Katsuyuki
  • Organizer: 日本免疫学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] IRF4 controls cytokine signals and plays critical roles for proliferation and differentiation of CD8+ T cells. (2012)
  • Author(s): MIYAKODA Mana, HONMA Kiri, KIMURA Daisuke, KIMURA Kazumi, MATSUYAMA Toshifumi, YUI Katsuyuki
  • Organizer: 日本免疫学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Th2細胞非依存的N. brasiliensis感染排除機構の解析―ナチュラルヘルパー細胞の解析 (2012)
  • Author(s): 本間季里
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫医科大学
• [Presentation] マラリア原虫感染によって引き起こされる CD4+ T 細胞の IL-2 産生抑制機序の解析 (2012)
  • Author(s): 木村大輔
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫医科大学
• [Presentation] マラリア原虫感染中にみられる抗原特異的記憶CD8+ T細胞の二次応答の抑制 (2012)
  • Author(s): 都田真奈
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫医科大学
• [Presentation] マラリア肝細胞期における抗原提示機構の解析 (2012)
  • Author(s): 木村一美
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫医科大学
• [Presentation] The role of IRF4 in skin dendritic cells during Leishmania major infection (2012)
  • Author(s): Akbari Masoud
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 兵庫医科大学
• [Presentation] Th2非依存的なN.brasiliensis感染排除機構の解析—小腸に浸潤している好酸球の解析 (2011)
  • Author(s): 本間季里、木村大輔、木村一美、成毛有紀、都田真奈、蔵重智美、中島正洋、松山俊文、由井克之
  • Organizer: 第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
  • Place of Presentation: 東京(p.88)
• [Presentation] マラリア原虫感染における特異的記憶CD8^+T細胞の分化 (2011)
  • Author(s): 都田真奈、木村大輔、本間季里、木村一美、油田正夫、由井克之
  • Organizer: 第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
  • Place of Presentation: 東京(p.95)
• [Presentation] モデル抗原組換えマラリア原虫を用いた肝細胞期防御免疫応答の解析 (2011)
  • Author(s): 木村一美、木村大輔、都田真奈、本間季里、田村隆彦、油田正夫、由井克之
  • Organizer: 第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
  • Place of Presentation: 東京(p.95)
• [Presentation] マラリア原虫感染により誘導されるCD4^+ T細胞はIL-2産生抑制性の新規サイトカインを発現する (2011)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、油田正夫、由井克之
  • Organizer: 第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
  • Place of Presentation: 東京(p.95)
• [Presentation] マラリア原虫感染によって誘導される制御性CD4+T細胞は液性因子によってIL-2産生を抑制する (2011)
  • Author(s): 木村大輔、都田真奈、本間季里、木村一美、由井克之
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録:p. 207)
• [Presentation] モデル抗原組換えマラリア原虫を用いた肝細胞期防御免疫応答の解析 (2011)
  • Author(s): KIMURA Kazumi、KIMURA Daisuke、MIYAKODA Mana、HONMA Kiri、TAMURA Takahiko、YUI Katsuyuki
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録:p. 49)
• [Presentation] IRF4 negatively regulates Th2-type cytokine production in natural helper cells (2011)
  • Author(s): Kiri Honma、Daisuke Kimura、Kazumi Kimura、Mana Miyakoda、Kazuyo Moro、Shigeo Koyasu、Toshifumi Matsuyama、Katsuyuki Yui
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録:p. 81)
• [Presentation] IRF-4欠損MRL/lprマウスはTh1過剰応答を起こし複数の組織に肉芽腫性病変を発症する (2011)
  • Author(s): Machida Takeshi、Honma Kiri、Matsuyama Toshifumi、Yui Katsuyuki、Ruiz Phil、Fujita Teizo、Gilkeson Gary、Sekine Hideharu
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉(学術集会記録:p. 84)
• [Presentation] Th2非依存的なN.brasiliensis感染排除機構の解析―小腸に浸潤している好酸球の解析 (2011)
  • Author(s): 本間季里
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 慈恵会医科大学
• [Presentation] マラリア原虫感染における特異的記憶CD8+T細胞の分化 (2011)
  • Author(s): 都田真奈
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 慈恵会医科大学
• [Presentation] マラリア原虫感染により誘導されるCD4+ T細胞はIL-2産生抑制性の新規サイトカインを発現する。 (2011)
  • Author(s): 木村大輔
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 慈恵会医科大学
• [Presentation] モデル抗原組換えマラリア原虫を用いた肝細胞期防御免疫応答の解析 (2011)
  • Author(s): 木村一美
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 慈恵会医科大学
• [Presentation] IRF4 negatively regulates Th2-type cytokine production in natural helper cells (2011)
  • Author(s): 本間季里
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] マラリア原虫感染によって誘導される制御性 CD4+T細胞は液性因子によってIL-2産生を抑制する (2011)
  • Author(s): 木村大輔
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] モデル抗原組換えマラリア原虫を用いた肝細 胞期防御免疫応答の解析 (2011)
  • Author(s): 木村一美
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] IRF-4欠損MRL/lprマウスはTh1過剰応答を起こし複数の組織に肉芽腫性病変を発症する (2011)
  • Author(s): 町田豪
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Book] 寄生虫学研究:材料と方法2012年版 (2012)
  • Author(s): 宇賀昭二、丸山治彦編
  • Publisher: 三恵社
• [Book] カラー版ミムス微生物学 (2012)
  • Author(s): 監訳中込治
  • Publisher: 西村書店
• [Book] ミムス 微生物学 (2012)
  • Author(s): 中込治・監訳
  • Total Pages: 606
  • Publisher: 西村書店