• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of human monoclonal antibodies to major surface antigens of parasitic protozoa for clinical applications

Research Project

Project/Area Number 23590496
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionTokai University

Principal Investigator

TACHIBANA Hiroshi  東海大学, 医学部, 教授 (10147168)

Research Collaborator CHENG Xunjia  復旦大学, 医学院, 教授
FU Yongfeng  東海大学, 医学院, 研究員
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords寄生原虫 / ヒトモノクローナル抗体 / 熱帯熱マラリア / 赤痢アメーバ症 / 抗体遺伝子 / 抗体医薬 / 表面抗原 / 熱帯熱マラリア原虫 / 赤痢アメーバ / ヒト抗体
Research Abstract

Whole human monoclonal IgG molecules were prepared from a Fab fragment specific to the C-terminus 19-kDa fragment of Plasmodium falciparum merozoite surface protein 1. Affinities of IgG1 and IgG3 molecules produced in CHO cells were approximately 20-fold higher than that of the original Fab fragment. Whole human IgG1 molecule was also prepared from a monoclonal antibody Fab fragment specific for surface lectin of Entamoeba histolytica. These whole human IgG molecules may be applicable for prevention, diagnosis and therapy of protozoan infectious diseases.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (17 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Correlation between genotypes of tRNA-linked short tandem repeats in Entamoeba nuttalli isolates and the geographical distribution of host rhesus macaques2014

    • Author(s)
      Meng Feng, Tomoyoshi Komiyama, Tetsuo Yanagi, Xunjia Cheng, Jeevan B. Sherchand and Hiroshi Tachibana
    • Journal Title

      Parasitol. Res.

      Volume: 113 Issue: 1 Pages: 367-374

    • DOI

      10.1007/s00436-013-3664-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates2013

    • Author(s)
      Hiroshi Tachibana, Tetsuo Yanagi, Chamala Lama, Kishor Pandey, Meng Feng, Seiki Kobayashi and Jeevan B. Sherchand
    • Journal Title

      Parasitol. Int.

      Volume: 62 Issue: 2 Pages: 230-235

    • DOI

      10.1016/j.parint.2013.01.004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of Entamoeba species infecting macaques in southwest China2013

    • Author(s)
      Meng Feng, Junlong Cai, Xiangyang Min, Yonggeng Fu, Qing Xu, Hiroshi Tachibana and Xunjia Cheng
    • Journal Title

      Parasitol. Res.

      Volume: 112 Issue: 4 Pages: 1529-1536

    • DOI

      10.1007/s00436-013-3299-1

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Primary pathogenicity analysis of a Chinese <i>Entamoeba histolytica </i>isolate2013

    • Author(s)
      Muxia Luo, Meng Feng, Xiangyang Min, Xueping Li, Junlong Cai, Hiroshi Tachibana and Xunjia Cheng
    • Journal Title

      BioScience Trends

      Volume: 7 Issue: 2 Pages: 77-81

    • DOI

      10.5582/bst.2013.v7.2.77

    • NAID

      130005054649

    • ISSN
      1881-7815, 1881-7823
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An integrated microfluidic device for rapid serodiagnosis of amebiasis2013

    • Author(s)
      Wang Zhao, Li Zhang, Wenwen Jing, Sixiu Liu, Hiroshi Tachibana, Xunjia Cheng, Guodong Sui
    • Journal Title

      Biomicrofluidics

      Volume: Vol.7 Issue: 1 Pages: 11101-11101

    • DOI

      10.1063/1.4793222

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Seroprevalence of Entamoeba histolytica infection in China2012

    • Author(s)
      Bin Yang, Yingdan Chen, Liang Wu, Longqi Xu, Hiroshi Tachibana, Xunjia Cheng
    • Journal Title

      Am. J. Trop. Med. Hyg.

      Volume: Vo.87 Issue: 1 Pages: 97-103

    • DOI

      10.4269/ajtmh.2012.11-0626

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Unique short tandem repeat nucleotide sequences in Entamoeba histolytica isolates from China2012

    • Author(s)
      Meng Feng, Junlong Cai, Bin Yang, Yongfeng Fu, Xiangyang Min, Hiroshi Tachibana, Xunjia Cheng
    • Journal Title

      Parasitol. Res.

      Volume: Vol.111 Issue: 3 Pages: 1137-1142

    • DOI

      10.1007/s00436-012-2945-3

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] High prevalence of Entamoeba infections in captive long-tailed macaques in China2011

    • Author(s)
      Feng M, Yang B, Yang L, Fu Y, Zhuang Y, Liang L, Xu Q, Cheng X, Tachibana H
    • Journal Title

      Parasitol.Res.

      Volume: 109 Issue: 4 Pages: 1093-1097

    • DOI

      10.1007/s00436-011-2351-2

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl)2014

    • Author(s)
      Kentaro Kato, Bhim G. Dhoubhadel, Yoshito Fujii, Hiroshi Tachibana
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2014-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Polymorphism in and antibody responses to thrombospondin-related adhesive protein of Plasmodium vivax (PvTRAP)2014

    • Author(s)
      Rattiporn Kosuwin, Meng Feng, Chaturong Putaporntip, Takashi Makiuchi, Hiroshi Tachibana, Somchai Jongwutiwes
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Correlation between tRNA-linked short tandem repeats genes in Entamoeba nuttalli isolates and the geographical distribution of host macaques2014

    • Author(s)
      Meng Feng, Tomoyoshi Komiyama, Tetsuo Yanagi, Xunjia Cheng, Jeevan B. Sherchand, Hiroshi Tachibana
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Amebicidal activity of artemether by inhibition of phosphoserine aminotransferase synthesis in Acanthamoeba castellanii2014

    • Author(s)
      Xunjia Cheng, Yihong Deng, Wei Ran, Suqin Man, Xueping Li, Hongjian Gao, Wei Tang, Hiroshi Tachibana
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 赤痢アメーバ症の診断近縁種との鑑別と多型解析2013

    • Author(s)
      橘裕司
    • Organizer
      静岡県寄生虫症研究会第13回研究総会
    • Place of Presentation
      アクトシティ浜松研修交流センター
    • Year and Date
      2013-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 野生のトクザルから分離したEntamoeba nuttalliの性状解析2012

    • Author(s)
      橘 裕司、柳 哲雄、小林正規、平山謙二、Rajapakse R. P. V. Jayanthe
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大学西宮キャンパス(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] スリランカのトクモンキーからのEntamoeba nuttalliの分離と性状解析2011

    • Author(s)
      橘 裕司、柳 哲雄、小林正規、平山謙二、Rajapakse R. P. V. Jayanthe
    • Organizer
      第52回日本熱帯医学会大会
    • Place of Presentation
      東京大学本郷キャンパス(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Polymorphism in and antibody responses to thrombospondin-related adhesisve protein of Plasmodium vivax (PvTRAP)

    • Author(s)
      Kosuwin Rattiporn 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Amebicidal activity of artemether by inhibition of phosphoserine aminotransferase synthesis in Acanthamoeba castellanii

    • Author(s)
      程訓佳 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl)

    • Author(s)
      加藤健太郎 他
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤痢アメーバ症の診断 近縁種との鑑別と多型解析

    • Author(s)
      橘裕司
    • Organizer
      静岡県寄生虫症研究会第13回研究総会
    • Place of Presentation
      アクトシティ浜松研修交流センター
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ネパールのカトマンズにおける腸管寄生アメーバ感染の疫学研究

    • Author(s)
      橘 裕司 他
    • Organizer
      第53回日本熱帯医学会大会
    • Place of Presentation
      とかちプラザ(北海道)
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of genotype information in Enamoeba histolytica isolates from China

    • Author(s)
      Feng M. et al.
    • Organizer
      第53回日本熱帯医学会大会
    • Place of Presentation
      とかちプラザ(北海道)
    • Related Report
      2012 Research-status Report
  • [Presentation] タイ中央部における野生カニクイザルからのEntamoeba nuttaliの分離とその性状解析

    • Author(s)
      橘 裕司 他
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Prevalence and genetic diversity of Entamoeba species infecting macaques in Southwest China

    • Author(s)
      Feng M. et al.
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] PCR法で診断されたアメーバ性肝膿瘍の2症例

    • Author(s)
      山田 稔 他
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Isolation and characterization of Enamoeba nuttalli from wild macaques in Asia

    • Author(s)
      Tachibana H.
    • Organizer
      International Workshop on Enteric Protozoan Infections
    • Place of Presentation
      国立感染症研究所(東京都)
    • Related Report
      2012 Research-status Report
  • [Book] Antibody Expression and Production, Production of antibody Fab fragments in Escherichia coli2011

    • Author(s)
      Hiroshi Tachibana, Masataka Takekoshi, (Al-Rubeai. M.(Ed.))
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] Chapter 8. Production of antibody Fab fragments in Escherichia coli. In: Al-Rubeai M(Ed.) Antibody Expression and Production. Cell Engineering 72011

    • Author(s)
      Tachibana H., Takekoshi M.
    • Publisher
      Springer
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.med.u-tokai.ac.jp/daigakuin/pdf/tatibana_hiroshi_2014_04_01.pdf

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi