Development of human monoclonal antibodies to major surface antigens of parasitic protozoa for clinical applications

• Project/Area Number: 23590496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tokai University
• Principal Investigator: TACHIBANA Hiroshi 東海大学, 医学部, 教授 (10147168)
• Research Collaborator: CHENG Xunjia 復旦大学, 医学院, 教授 ; FU Yongfeng 東海大学, 医学院, 研究員
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 寄生原虫 / ヒトモノクローナル抗体 / 熱帯熱マラリア / 赤痢アメーバ症 / 抗体遺伝子 / 抗体医薬 / 表面抗原 / 熱帯熱マラリア原虫 / 赤痢アメーバ / ヒト抗体

Research Abstract

Whole human monoclonal IgG molecules were prepared from a Fab fragment specific to the C-terminus 19-kDa fragment of Plasmodium falciparum merozoite surface protein 1. Affinities of IgG1 and IgG3 molecules produced in CHO cells were approximately 20-fold higher than that of the original Fab fragment. Whole human IgG1 molecule was also prepared from a monoclonal antibody Fab fragment specific for surface lectin of Entamoeba histolytica. These whole human IgG molecules may be applicable for prevention, diagnosis and therapy of protozoan infectious diseases.

Research Products

• [Journal Article] Correlation between genotypes of tRNA-linked short tandem repeats in Entamoeba nuttalli isolates and the geographical distribution of host rhesus macaques (2014)
  • Author(s): Meng Feng, Tomoyoshi Komiyama, Tetsuo Yanagi, Xunjia Cheng, Jeevan B. Sherchand and Hiroshi Tachibana
  • Journal Title: Parasitol. Res. Volume: 113 Issue: 1 Pages: 367-374
  • DOI: 10.1007/s00436-013-3664-0
  • Peer Reviewed
• [Journal Article] Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates (2013)
  • Author(s): Hiroshi Tachibana, Tetsuo Yanagi, Chamala Lama, Kishor Pandey, Meng Feng, Seiki Kobayashi and Jeevan B. Sherchand
  • Journal Title: Parasitol. Int. Volume: 62 Issue: 2 Pages: 230-235
  • DOI: 10.1016/j.parint.2013.01.004
  • Peer Reviewed
• [Journal Article] Prevalence and genetic diversity of Entamoeba species infecting macaques in southwest China (2013)
  • Author(s): Meng Feng, Junlong Cai, Xiangyang Min, Yonggeng Fu, Qing Xu, Hiroshi Tachibana and Xunjia Cheng
  • Journal Title: Parasitol. Res. Volume: 112 Issue: 4 Pages: 1529-1536
  • DOI: 10.1007/s00436-013-3299-1
  • Peer Reviewed
• [Journal Article] Primary pathogenicity analysis of a Chinese <i>Entamoeba histolytica </i>isolate (2013)
  • Author(s): Muxia Luo, Meng Feng, Xiangyang Min, Xueping Li, Junlong Cai, Hiroshi Tachibana and Xunjia Cheng
  • Journal Title: BioScience Trends Volume: 7 Issue: 2 Pages: 77-81
  • DOI: 10.5582/bst.2013.v7.2.77
  • NAID: 130005054649
  • ISSN: 1881-7815, 1881-7823
  • Peer Reviewed
• [Journal Article] An integrated microfluidic device for rapid serodiagnosis of amebiasis (2013)
  • Author(s): Wang Zhao, Li Zhang, Wenwen Jing, Sixiu Liu, Hiroshi Tachibana, Xunjia Cheng, Guodong Sui
  • Journal Title: Biomicrofluidics Volume: Vol.7 Issue: 1 Pages: 11101-11101
  • DOI: 10.1063/1.4793222
  • Peer Reviewed
• [Journal Article] Seroprevalence of Entamoeba histolytica infection in China (2012)
  • Author(s): Bin Yang, Yingdan Chen, Liang Wu, Longqi Xu, Hiroshi Tachibana, Xunjia Cheng
  • Journal Title: Am. J. Trop. Med. Hyg. Volume: Vo.87 Issue: 1 Pages: 97-103
  • DOI: 10.4269/ajtmh.2012.11-0626
  • Peer Reviewed
• [Journal Article] Unique short tandem repeat nucleotide sequences in Entamoeba histolytica isolates from China (2012)
  • Author(s): Meng Feng, Junlong Cai, Bin Yang, Yongfeng Fu, Xiangyang Min, Hiroshi Tachibana, Xunjia Cheng
  • Journal Title: Parasitol. Res. Volume: Vol.111 Issue: 3 Pages: 1137-1142
  • DOI: 10.1007/s00436-012-2945-3
  • Peer Reviewed
• [Journal Article] High prevalence of Entamoeba infections in captive long-tailed macaques in China (2011)
  • Author(s): Feng M, Yang B, Yang L, Fu Y, Zhuang Y, Liang L, Xu Q, Cheng X, Tachibana H
  • Journal Title: Parasitol.Res. Volume: 109 Issue: 4 Pages: 1093-1097
  • DOI: 10.1007/s00436-011-2351-2
  • Peer Reviewed
• [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl) (2014)
  • Author(s): Kentaro Kato, Bhim G. Dhoubhadel, Yoshito Fujii, Hiroshi Tachibana
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2014-12-04
• [Presentation] Polymorphism in and antibody responses to thrombospondin-related adhesive protein of Plasmodium vivax (PvTRAP) (2014)
  • Author(s): Rattiporn Kosuwin, Meng Feng, Chaturong Putaporntip, Takashi Makiuchi, Hiroshi Tachibana, Somchai Jongwutiwes
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
  • Year and Date: 2014-03-28
• [Presentation] Correlation between tRNA-linked short tandem repeats genes in Entamoeba nuttalli isolates and the geographical distribution of host macaques (2014)
  • Author(s): Meng Feng, Tomoyoshi Komiyama, Tetsuo Yanagi, Xunjia Cheng, Jeevan B. Sherchand, Hiroshi Tachibana
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
  • Year and Date: 2014-03-28
• [Presentation] Amebicidal activity of artemether by inhibition of phosphoserine aminotransferase synthesis in Acanthamoeba castellanii (2014)
  • Author(s): Xunjia Cheng, Yihong Deng, Wei Ran, Suqin Man, Xueping Li, Hongjian Gao, Wei Tang, Hiroshi Tachibana
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
  • Year and Date: 2014-03-27
• [Presentation] 赤痢アメーバ症の診断近縁種との鑑別と多型解析 (2013)
  • Author(s): 橘裕司
  • Organizer: 静岡県寄生虫症研究会第13回研究総会
  • Place of Presentation: アクトシティ浜松研修交流センター
  • Year and Date: 2013-09-14
• [Presentation] 野生のトクザルから分離したEntamoeba nuttalliの性状解析 (2012)
  • Author(s): 橘 裕司、柳 哲雄、小林正規、平山謙二、Rajapakse R. P. V. Jayanthe
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学西宮キャンパス（兵庫県）
• [Presentation] スリランカのトクモンキーからのEntamoeba nuttalliの分離と性状解析 (2011)
  • Author(s): 橘 裕司、柳 哲雄、小林正規、平山謙二、Rajapakse R. P. V. Jayanthe
  • Organizer: 第52回日本熱帯医学会大会
  • Place of Presentation: 東京大学本郷キャンパス（東京都）
• [Presentation] Polymorphism in and antibody responses to thrombospondin-related adhesisve protein of Plasmodium vivax (PvTRAP)
  • Author(s): Kosuwin Rattiporn 他
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] Amebicidal activity of artemether by inhibition of phosphoserine aminotransferase synthesis in Acanthamoeba castellanii
  • Author(s): 程訓佳 他
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl)
  • Author(s): 加藤健太郎 他
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 赤痢アメーバ症の診断 近縁種との鑑別と多型解析
  • Author(s): 橘裕司
  • Organizer: 静岡県寄生虫症研究会第13回研究総会
  • Place of Presentation: アクトシティ浜松研修交流センター
  • Invited
• [Presentation] ネパールのカトマンズにおける腸管寄生アメーバ感染の疫学研究
  • Author(s): 橘 裕司 他
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ（北海道）
• [Presentation] Analysis of genotype information in Enamoeba histolytica isolates from China
  • Author(s): Feng M. et al.
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ（北海道）
• [Presentation] タイ中央部における野生カニクイザルからのEntamoeba nuttaliの分離とその性状解析
  • Author(s): 橘 裕司 他
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学湯島キャンパス（東京都）
• [Presentation] Prevalence and genetic diversity of Entamoeba species infecting macaques in Southwest China
  • Author(s): Feng M. et al.
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学湯島キャンパス（東京都）
• [Presentation] PCR法で診断されたアメーバ性肝膿瘍の2症例
  • Author(s): 山田 稔 他
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学湯島キャンパス（東京都）
• [Presentation] Isolation and characterization of Enamoeba nuttalli from wild macaques in Asia
  • Author(s): Tachibana H.
  • Organizer: International Workshop on Enteric Protozoan Infections
  • Place of Presentation: 国立感染症研究所（東京都）
• [Book] Antibody Expression and Production, Production of antibody Fab fragments in Escherichia coli (2011)
  • Author(s): Hiroshi Tachibana, Masataka Takekoshi, (Al-Rubeai. M.(Ed.))
  • Publisher: Springer
• [Book] Chapter 8. Production of antibody Fab fragments in Escherichia coli. In: Al-Rubeai M（Ed.） Antibody Expression and Production. Cell Engineering 7 (2011)
  • Author(s): Tachibana H., Takekoshi M.
  • Publisher: Springer
• [Remarks]
  • URL: http://www.med.u-tokai.ac.jp/daigakuin/pdf/tatibana_hiroshi_2014_04_01.pdf