Functional gene analysis for the influence of the indwelling catheter in the Candida ifection

• Project/Area Number: 23590502
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Chiba University
• Principal Investigator: CHIBANA Hiroji 千葉大学, 真菌医学研究センター, 准教授 (30333488)
• Co-Investigator(Kenkyū-buntansha): UNO Jun 千葉大学, 真菌医学研究センター, 特任研究員 (40114243)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 病原真菌 / 病原性 / 遺伝子操作 / 国際情報交換 / ポルトガル / ゲノム / 真菌 / フェノーム / 機能ゲノミクス

Research Abstract

It is thought that multiple factors participate in pathogenicity of the Candida globrata, but there are many unexplained points. In a recent study, it becomes the problem in particular as a risk factor is indwelling catheter. Therefore we made deletion mutants of the genes encoding beta-glucan, manno protein, the secretase such as an ingredient or the hydrolase which constituted the cell outer layer including the lectin. They were thought that participating in adhesion of the indwelling catheter by the formation of the biofilm.

Research Products

• [Journal Article] Genome-wide survey of transcriptional initiation in the pathogenic fungus, Candida glabrata (2014)
  • Author(s): Toshihiro Aoyama, Hironobu Nakayama, Keigo Ueno K, Tatsuya Inukai T, Koichi Tanabe, Minoru Nagi, Martin M. Bard Hiroji Chibana
  • Journal Title: Genes Cells Volume: (in press) Issue: 6 Pages: 478-503
  • DOI: 10.1111/gtc.12147
  • Peer Reviewed
• [Journal Article] The Candida glabrata sterol scavenging mechanism, mediated by the ATP-binding cassette transporter Aus1p, is regulated by iron limitation. (2013)
  • Author(s): Nagi M, Tanabe K, Ueno K, Nakayama H, Aoyama T, Chibana H, Yamagoe S, Umeyama T, Oura T, Ohno H, Kajiwara S, Miyazaki Y.
  • Journal Title: Mol Microbiol. Volume: 88 Issue: 2 Pages: 371-81
  • DOI: 10.1111/mmi.12189
  • Peer Reviewed
• [Presentation] Candida glabrata遺伝子改変株コレクションを用いた病原性の理解と抗真菌薬の標的探索 (2013)
  • Author(s): 知花博治
  • Organizer: 第57回医真菌学会総会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-09-27
  • Invited
• [Presentation] Research project for understanding phathogenicity and parasitism of Candida glabrata (2013)
  • Author(s): Chibana H
  • Organizer: The 20 th anniversary symposium. The Korean Society for Medical Mycology
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 2013-06-01
  • Invited
• [Presentation] Research project for understanding phathogenicity and parasitism of Candida glabrata (2013)
  • Author(s): Hiroji Chibana
  • Organizer: The 20 th The Korean Society for Medical Mycology. Seoul.
  • Place of Presentation: 韓国ソウル
  • Invited