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Investigation of the regulatory mechanism for the production of a human specific cytolysin, a crucial factor for the infectivity of Streptococcus intermedius

Research Project

Project/Area Number 23590510
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionThe University of Tokushima

Principal Investigator

TOMOYASU Toshifumi  徳島大学, ソシオテクノサイエンス研究部, 准教授 (20323404)

Co-Investigator(Kenkyū-buntansha) TABATA Atushi  徳島大学, 大学院ソシオテクノサイエンス研究部, 助教 (10432767)
NAGAMUNE Hideaki  徳島大学, 大学院ソシオテクノサイエンス研究部, 教授 (40189163)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords連鎖球菌 / 細胞溶解毒素 / 発現制御 / 細胞毒性 / ガラクトース / 病原因子 / グリコシダーゼ / 糖鎖 / CcpA / LacR / 発現調節機構 / 病原性
Research Abstract

Streptococcus intermedius secretes intermedilysin(ILY), which is a major virulence factor of this pathogen. We showed that the disruption of lacR in an ILY low-producing strain (PC574) led to higher hemolytic activity, increased transcription of ily and higher cytotoxicity against HepG2 cells, when compared to the parental strain. The direct binding of LacR within the ily promoter region was observed by a DNA probe pull-down assay. Adding galactose or lactose into the medium increased the amount of ILY secreted by PC574 cells.
Furthermore, we studied the lacR nucleotide sequences and the hemolytic activity of 50 strains isolated from clinical infections and 7 strains isolated from dental plaque. Thirteen strains isolated from clinical infections showed high ILY production and 9 strains had one or more point mutations and/or an insertion mutation in LacR, which caused the decline in its function.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (35 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (33 results)

  • [Journal Article] LacR mutations are frequently observed in Streptococcus intermedius and are responsible for increased intermedilysin production and virulence.2013

    • Author(s)
      Tomoyasu T, Imaki H, Masuda S, Okamoto A, Kim HJ, Waite RD, Whiley RA, Kikuchi K, Hiramatsu K, Tabata A and Nagamune H.
    • Journal Title

      Infect. Immun.

      Volume: 81 Issue: 9 Pages: 3276-3286

    • DOI

      10.1128/iai.00638-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Streptococcus intermedius DnaK chaperone system in stress tolerance and pathogenicity2012

    • Author(s)
      Tomoyasu T, Tabata A, Imaki H, Tsuruno K, Miyazaki A, Sonomoto K, Whiley RA, Nagamune H
    • Journal Title

      Cell Stress Chaperones

      Volume: 17(1) Issue: 1 Pages: 41-55

    • DOI

      10.1007/s12192-011-0284-4

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Characterization of a novel secreted glycosidase (MsgA) of Streptococcus intermedius2014

    • Author(s)
      山本直輝
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2014-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification and study of the binding region of ily transcriptional repressive element2014

    • Author(s)
      友安俊文、金子幸広、田端厚之、長宗秀明
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀 (東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of a novel secreted glycosidase (MsgA) of Streptococcus intermedius2014

    • Author(s)
      山本直樹、友安俊文、今木英統、田端厚之、長宗秀明
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀 (東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification and characterization of MsgA, a novel secreted glycosidase of Streptococcus intermedius2013

    • Author(s)
      友安俊文
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Streptococcus intermediusの主要病原因子であるインターメディリシンの発現調節機構の解析2013

    • Author(s)
      友安俊文
    • Organizer
      第60回毒素シンポジウム
    • Place of Presentation
      楓香荘(兵庫県)
    • Year and Date
      2013-07-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖タンパク質によるStreptococcus intermediusのily発現促進機構の解析2013

    • Author(s)
      今木英統
    • Organizer
      第22回Lancefieldレンサ球菌研究会
    • Place of Presentation
      島根イン青山(東京都)
    • Year and Date
      2013-06-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Streptococcus intermediusのily発現促進因子の同定2013

    • Author(s)
      今木英統
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ国際会議場(千葉県)
    • Year and Date
      2013-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖タンパク質によるStreptococcus intermediusのily発現促進機構の解析2013

    • Author(s)
      今木英統、友安俊文、増田早智子、山本直輝、田端厚之、長宗秀明
    • Organizer
      第22回Lancefieldレンサ球菌研究会
    • Place of Presentation
      島根イン青山 (東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Streptococcus intermedius の主要病原因子であるインターメディリシンの発現調節機構の解析2013

    • Author(s)
      友安俊文、今木英統、山本直輝、田端厚之、長宗秀明
    • Organizer
      第60回毒素シンポジウム
    • Place of Presentation
      楓香荘 (兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Streptococcus intermediusが分泌する新規グリコシダーゼの発見と性状解析2013

    • Author(s)
      山本直輝、友安俊文、今木英統、田端厚之、長宗秀明
    • Organizer
      第66回日本細菌学会中国・四国支部総会
    • Place of Presentation
      広島国際大学 (広島県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification and characterization of MsgA, a novel secreted glycosidase of Streptococcus intermedius2013

    • Author(s)
      友安俊文、今木英統、山本直輝、田端厚之、長宗秀明
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド (兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ily転写抑制因子の結合領域の探索2013

    • Author(s)
      金子 幸広 他
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Research-status Report
  • [Presentation] ウシ胎児血清に存在するStreptococcus intermediusの ily発現活性化因子の同定2013

    • Author(s)
      今木 英統 他
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Research-status Report
  • [Presentation] ILYを高産生するStreptococcus intermediusの臨床分離株の多くがLacRに変異を持つ2012

    • Author(s)
      今木英統
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Year and Date
      2012-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] TOMM family peptides decide the hemolytic character of Streptococcus anginosus2012

    • Author(s)
      田端 厚之
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] ILYを高産生するStreptococcus intermediusの臨床分離株の多くがLacRに変異を持つ2012

    • Author(s)
      今木 英統
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] グラム陽性菌と陰性菌のコシャペロンによるDnaK活性化機構の比較2012

    • Author(s)
      友安 俊文
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular and phenotypic analysis of mutants of ily expressional control factors in Streptococcus intermedius2011

    • Author(s)
      Hidenori Imaki
    • Organizer
      IUMS 2011 Sapporo Congress
    • Place of Presentation
      Sapporo Convention Center (Sapporo, Japan)
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic screening of ily expression control factors in Streptococcus intermedius2011

    • Author(s)
      Toshifumi Tomoyasu
    • Organizer
      XVIII Lancefield International Symposium
    • Place of Presentation
      Hilton Villa Igiea (Palermo, Italy)
    • Year and Date
      2011-09-07
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] コレステロール依存性細胞溶解毒素intermedilysinの発現調節因子がStreptococcus intermediusの病原性に果たす役割の解析2011

    • Author(s)
      友安俊文
    • Organizer
      第58回トキシンシンポジウム
    • Place of Presentation
      順天堂大学本郷キャンパス(東京都)
    • Year and Date
      2011-07-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Investigation of pathogenic properties of cholesterol-dependent cytolysin producing Streptococcus mitis2011

    • Author(s)
      Atsushi Tabata
    • Organizer
      XVIII Lancefield International Symposium
    • Place of Presentation
      Hilton Villa Igiea (Palermo, Italy)
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular and phenotypic analysis of mutants of ily expressional control factors in Streptococcus intermedius2011

    • Author(s)
      Hidenori Imaki
    • Organizer
      IUMS 2011 Sapporo Congress
    • Place of Presentation
      Sapporo Convention Center(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Investigation of the novel cytotoxic factor secreted from Streptococcus anginosus2011

    • Author(s)
      Kota Nakano
    • Organizer
      IUMS 2011 Sapporo Congress
    • Place of Presentation
      Sapporo Convention Center(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional analysis of Streptococcus intermedius DnaK system in vitro2011

    • Author(s)
      Aya Miyazaki
    • Organizer
      The 8th International AFAS Joint Symposium Between Japan and Korea
    • Place of Presentation
      Yonago convention center(鳥取県)
    • Related Report
      2011 Research-status Report
  • [Presentation] アンギノーサス群連鎖球菌が保有する溶血因子の探索2011

    • Author(s)
      田端 厚之
    • Organizer
      第20回 Lancefieldレンサ球菌研究会
    • Place of Presentation
      愛知学院大学薬学部(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] コレステロール依存性細胞溶解毒素intermedilysinの発現調節因子がStreptococcus intermediusの病原性に果たす役割の解析2011

    • Author(s)
      友安 俊文
    • Organizer
      第58回 トキシンシンポジウム
    • Place of Presentation
      順天堂大学本郷キャンパス(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] アンギノーサス群連鎖球菌が保有する溶血因子に関する検討2011

    • Author(s)
      田端 厚之
    • Organizer
      第5回 細菌学若手コロッセウム
    • Place of Presentation
      高知大学農学部(高知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Streptococcus intermediusのily発現調節因子の探索2011

    • Author(s)
      今木 英統
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Related Report
      2011 Research-status Report
  • [Presentation] β溶血性Streptococcus anginosusで見出された新規ペプチド性溶血因子2011

    • Author(s)
      中野 晃太
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Streptococcus intermediusが保有するDnaKシャペロンシステムの機能解析2011

    • Author(s)
      友安 俊文
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Streptococcus intermedius DnaK システムのin vitroにおける機能解析2011

    • Author(s)
      宮崎 彩
    • Organizer
      第18回日本生物工学会九州支部福岡大会
    • Place of Presentation
      九州大学(福岡県)
    • Related Report
      2011 Research-status Report
  • [Presentation] LacRはStreptococcus intermediusのily発現の主要な制御因子である

    • Author(s)
      今木英統 他
    • Organizer
      第21回 Lancefield レンサ球菌研究会
    • Place of Presentation
      大阪大学 吹田キャンパス内 弓倉記念ホール(大阪府)
    • Related Report
      2012 Research-status Report
  • [Presentation] ily 発現抑制因子が認識するコンセンサス配列の探索

    • Author(s)
      金子 幸広 他
    • Organizer
      第65回日本細菌学会中国・四国支部総会
    • Place of Presentation
      徳島大学 蔵本キャンパス内 長井記念ホール(徳島県)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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